ATE141649T1 - Verfahren zum nachweis von hydrolytischen enzymen - Google Patents
Verfahren zum nachweis von hydrolytischen enzymenInfo
- Publication number
- ATE141649T1 ATE141649T1 AT92305109T AT92305109T ATE141649T1 AT E141649 T1 ATE141649 T1 AT E141649T1 AT 92305109 T AT92305109 T AT 92305109T AT 92305109 T AT92305109 T AT 92305109T AT E141649 T1 ATE141649 T1 AT E141649T1
- Authority
- AT
- Austria
- Prior art keywords
- resin
- binding
- assay method
- scissile bond
- plates
- Prior art date
Links
- 102000004190 Enzymes Human genes 0.000 title abstract 3
- 108090000790 Enzymes Proteins 0.000 title abstract 3
- 230000003301 hydrolyzing effect Effects 0.000 title abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 3
- 239000000758 substrate Substances 0.000 abstract 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 abstract 2
- 238000003556 assay Methods 0.000 abstract 2
- 239000011347 resin Substances 0.000 abstract 2
- 229920005989 resin Polymers 0.000 abstract 2
- 108010016183 Human immunodeficiency virus 1 p16 protease Proteins 0.000 abstract 1
- 102000000422 Matrix Metalloproteinase 3 Human genes 0.000 abstract 1
- 108091005804 Peptidases Proteins 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 abstract 1
- 239000012190 activator Substances 0.000 abstract 1
- -1 biotin Chemical class 0.000 abstract 1
- 229960002685 biotin Drugs 0.000 abstract 1
- 235000020958 biotin Nutrition 0.000 abstract 1
- 239000011616 biotin Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 238000011534 incubation Methods 0.000 abstract 1
- 239000003112 inhibitor Substances 0.000 abstract 1
- 239000003550 marker Substances 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
- 239000012508 resin bead Substances 0.000 abstract 1
- 108091007196 stromelysin Proteins 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/22—Tachykinins, e.g. Eledoisins, Substance P; Related peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16211—Human Immunodeficiency Virus, HIV concerning HIV gagpol
- C12N2740/16222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
- G01N2333/155—Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
- G01N2333/16—HIV-1, HIV-2
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Toxicology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Virology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US07/712,828 US5235039A (en) | 1991-06-10 | 1991-06-10 | Substrates for hiv protease |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE141649T1 true ATE141649T1 (de) | 1996-09-15 |
Family
ID=24863712
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT92305109T ATE141649T1 (de) | 1991-06-10 | 1992-06-03 | Verfahren zum nachweis von hydrolytischen enzymen |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US5235039A (de) |
| EP (1) | EP0518557B1 (de) |
| JP (1) | JPH05194591A (de) |
| AT (1) | ATE141649T1 (de) |
| CA (1) | CA2070719A1 (de) |
| DE (1) | DE69212911D1 (de) |
| DK (1) | DK0518557T3 (de) |
Families Citing this family (48)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ249789A (en) * | 1992-03-11 | 1997-07-27 | Narhex Ltd | Hydrazine, carbazate and 1,2-diazacyclic derivatives and pharmaceutical compositions |
| US5888992A (en) * | 1992-03-11 | 1999-03-30 | Narhex Limited | Polar substituted hydrocarbons |
| US6071895A (en) * | 1992-03-11 | 2000-06-06 | Narhex Limited | Polar-substituted hydrocarbons |
| MXPA93002392A (es) | 1992-03-11 | 2005-02-04 | Narhex Ltd | Derivados amino de hidrocarburos-oxo e hidroxi-substituidos. |
| DE4217474A1 (de) * | 1992-05-27 | 1993-12-02 | Boehringer Mannheim Gmbh | Verfahren und Mittel zur Bestimmung eines Analyts |
| US5846993A (en) * | 1992-12-22 | 1998-12-08 | Agouron Pharmaceuticals, Inc. | HIV protease inhibitors |
| US5484926A (en) * | 1993-10-07 | 1996-01-16 | Agouron Pharmaceuticals, Inc. | HIV protease inhibitors |
| US6348310B1 (en) * | 1994-03-04 | 2002-02-19 | Promega Corporation | Quantitation of individual protein kinase activity |
| ATE165168T1 (de) * | 1994-03-29 | 1998-05-15 | Lepetit Spa | Festphasenimmunoassay zum nachweis von inhibitoren von einem proteolytischen enzym |
| US5527829A (en) * | 1994-05-23 | 1996-06-18 | Agouron Pharmaceuticals, Inc. | HIV protease inhibitors |
| JPH10506795A (ja) * | 1994-10-07 | 1998-07-07 | ロックフェラー・ユニバーシティ | グラム陽性菌からの表面蛋白のアンカー領域を開裂する酵素 |
| JP2000503533A (ja) * | 1996-01-04 | 2000-03-28 | ネーデルランドセ・オルガニザテイエ・フール・テゲパスト―ナトウールベテンシヤツペリーク・オンデルツエク・テイエヌオー | 蛍光消光基質を用いたタンパク質分解酵素のアッセイ方法 |
| CA2252799C (en) | 1996-04-30 | 2008-09-16 | De Novo Enzyme Corporation | Antiviral ricin-like proteins |
| US5962725A (en) | 1996-09-05 | 1999-10-05 | Agouron Pharmaceuticals, Inc. | Intermediate compounds useful for making HIV protease inhibitors such as nelfinavir |
| US5705647A (en) * | 1996-09-05 | 1998-01-06 | Agouron Pharmaceuticals, Inc. | Intermediates for making HIV-protease inhibitors |
| US5925759A (en) | 1996-09-05 | 1999-07-20 | Agouron Pharmaceuticals, Inc. | Methods of making HIV-protease inhibitors and intermediates for making HIV-protease inhibitors |
| US6001851A (en) * | 1997-03-13 | 1999-12-14 | Agouron Pharmaceuticals, Inc. | HIV protease inhibitors |
| EP0980440B1 (de) * | 1997-05-01 | 2007-08-01 | Minnesota Mining And Manufacturing Company | Fluorogene protease-substrate basierend auf farbstoffdimerisierung |
| US6084107A (en) * | 1997-09-05 | 2000-07-04 | Agouron Pharmaceuticals, Inc. | Intermediates for making HIV-protease inhibitors |
| US7745142B2 (en) | 1997-09-15 | 2010-06-29 | Molecular Devices Corporation | Molecular modification assays |
| WO2000075167A2 (en) * | 1999-06-09 | 2000-12-14 | Ljl Biosystems, Inc. | Phosphorylation assays |
| US6908770B1 (en) | 1998-07-16 | 2005-06-21 | Board Of Regents, The University Of Texas System | Fluid based analysis of multiple analytes by a sensor array |
| US6312896B1 (en) * | 1998-09-17 | 2001-11-06 | Igen Inaternational, Inc. | Assays for measuring nucleic acid binding proteins and enzyme activities |
| FR2786788B1 (fr) * | 1998-12-08 | 2002-04-19 | Proteus | Procede de criblage de substances capables de modifier l'activite d'une ou plusieurs proteines cibles ou d'un ensemble cible de proteines exprimees in vitro |
| FR2786787B1 (fr) | 1998-12-08 | 2002-04-19 | Proteus | Methode d'analyse in vitro d'un phenotype connu a partir d'un echantillon d'acides nucleiques |
| FR2786789B1 (fr) * | 1998-12-08 | 2001-12-28 | Proteus | Methode de detection in vitro d'une sequence d'acide nucleique cible dans un echantillon d'acide nucleique |
| US6911307B1 (en) | 1998-12-08 | 2005-06-28 | Proteus S.A. | Method of detection in vitro of a target substance in a sample comprising the labelling of said substance with a reporter gene and with the sequences necessary for the expression of said reporter gene in vitro |
| US6531125B1 (en) | 1999-03-02 | 2003-03-11 | Twinstrand Therapeutics Inc. | Antiviral ricin-like proteins |
| CA2270639A1 (en) * | 1999-05-10 | 2000-11-10 | Zhibo Gan | A method for measuring biomolecules |
| EP1185712B8 (de) | 1999-05-28 | 2005-10-19 | Virco Bvba | Neue mutationsprofile der hiv-1 reverse transcriptase in verbindung mit phänotypischem medikamentresistenz |
| DE60031988T2 (de) * | 1999-07-16 | 2007-06-14 | The Board Of Regents Of The University Of Texas System, Austin | Verfahren und vorrichtung zur zuführung von proben zu einer chemischen sensormatrix |
| GB9929674D0 (en) | 1999-12-15 | 2000-02-09 | Novartis Res Found | Protease-activated receptor (PAR) modulator assays |
| US6713298B2 (en) | 2000-01-31 | 2004-03-30 | Board Of Regents, The University Of Texas System | Method and apparatus for the delivery of samples to a chemical sensor array |
| WO2002033638A2 (en) | 2000-10-20 | 2002-04-25 | Virco Bvba | Mutational profiles in hiv-1 reverse transcriptase correlated with phenotypic drug resistance |
| US6818396B1 (en) | 2000-11-28 | 2004-11-16 | Proteus S.A. | Process for determination of the activity of a substance using an in vitro functional test |
| WO2003023360A2 (en) * | 2001-09-10 | 2003-03-20 | Meso Scale Technologies, Llc | Methods and apparatus for conducting multiple measurements on a sample |
| EP1446498B1 (de) | 2001-11-08 | 2008-03-12 | Tibotec Pharmaceuticals Ltd. | Protease assay zur kontrolle medikamentöser therapie |
| US20070015230A1 (en) * | 2002-04-15 | 2007-01-18 | Hammond David J | Identification and characterization of analytes from whole blood |
| AU2003231731A1 (en) * | 2002-04-15 | 2003-11-03 | American National Red Cross | Method for detecting ligands and targets in a mixture |
| US8257967B2 (en) | 2002-04-26 | 2012-09-04 | Board Of Regents, The University Of Texas System | Method and system for the detection of cardiac risk factors |
| DE60321466D1 (de) | 2002-07-01 | 2008-07-17 | Tibotec Pharm Ltd | Mit phänotypischer medikamentenresistenz korrelierte mutationsprofile in hiv-1-reverse-transkriptase |
| AU2003254500B2 (en) | 2002-07-01 | 2009-11-19 | Tibotec Pharmaceuticals Ltd. | Mutational profiles in HIV-1 protease correlated with phenotypic drug resistance |
| WO2005059551A2 (en) | 2003-12-11 | 2005-06-30 | Board Of Regents, The University Of Texas System | Method and system for the analysis of saliva using a sensor array |
| US8101431B2 (en) | 2004-02-27 | 2012-01-24 | Board Of Regents, The University Of Texas System | Integration of fluids and reagents into self-contained cartridges containing sensor elements and reagent delivery systems |
| US8105849B2 (en) | 2004-02-27 | 2012-01-31 | Board Of Regents, The University Of Texas System | Integration of fluids and reagents into self-contained cartridges containing sensor elements |
| US7781226B2 (en) | 2004-02-27 | 2010-08-24 | The Board Of Regents Of The University Of Texas System | Particle on membrane assay system |
| WO2007053186A2 (en) | 2005-05-31 | 2007-05-10 | Labnow, Inc. | Methods and compositions related to determination and use of white blood cell counts |
| CN105136699B (zh) * | 2015-08-25 | 2018-03-16 | 四川大学 | 基于未变性染色皮粉底物的蛋白酶胶原蛋白水解活力测定方法及其应用 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU563827B2 (en) * | 1982-07-01 | 1987-07-23 | Millipore Corp. | Filtration apparatus |
| US4569907A (en) * | 1984-01-16 | 1986-02-11 | Monsanto Company | Thiopeptolide substrates for vertebrate collagenase |
| DE3531778A1 (de) * | 1985-09-06 | 1987-03-12 | Behringwerke Ag | Verfahren zur bestimmung von proteaseinhibitoren |
| CA1333883C (en) * | 1988-04-07 | 1995-01-10 | Shashidhara H. M. Murthy | Immunoassay utilizing biotin bridge with universal solid phase |
| DE3842197A1 (de) * | 1988-12-15 | 1990-06-21 | Hoechst Ag | Rasch spaltbares substrat fuer die hiv-protease |
-
1991
- 1991-06-10 US US07/712,828 patent/US5235039A/en not_active Expired - Fee Related
-
1992
- 1992-06-03 AT AT92305109T patent/ATE141649T1/de not_active IP Right Cessation
- 1992-06-03 EP EP92305109A patent/EP0518557B1/de not_active Expired - Lifetime
- 1992-06-03 DE DE69212911T patent/DE69212911D1/de not_active Expired - Lifetime
- 1992-06-03 DK DK92305109.8T patent/DK0518557T3/da active
- 1992-06-08 CA CA002070719A patent/CA2070719A1/en not_active Abandoned
- 1992-06-08 JP JP4147281A patent/JPH05194591A/ja not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| DE69212911D1 (de) | 1996-09-26 |
| EP0518557B1 (de) | 1996-08-21 |
| EP0518557A2 (de) | 1992-12-16 |
| CA2070719A1 (en) | 1992-12-11 |
| EP0518557A3 (en) | 1993-06-16 |
| JPH05194591A (ja) | 1993-08-03 |
| US5235039A (en) | 1993-08-10 |
| DK0518557T3 (da) | 1996-09-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |