AR103172A1 - Reducción selectiva de residuos de cisteina en anticuerpos il-17 - Google Patents
Reducción selectiva de residuos de cisteina en anticuerpos il-17Info
- Publication number
- AR103172A1 AR103172A1 ARP150104191A ARP150104191A AR103172A1 AR 103172 A1 AR103172 A1 AR 103172A1 AR P150104191 A ARP150104191 A AR P150104191A AR P150104191 A ARP150104191 A AR P150104191A AR 103172 A1 AR103172 A1 AR 103172A1
- Authority
- AR
- Argentina
- Prior art keywords
- antibodies
- antigen
- secukinumab
- preparation
- binding fragments
- Prior art date
Links
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 title 1
- 235000018417 cysteine Nutrition 0.000 title 1
- 239000002699 waste material Substances 0.000 title 1
- 238000002360 preparation method Methods 0.000 abstract 6
- 239000000427 antigen Substances 0.000 abstract 4
- 102000036639 antigens Human genes 0.000 abstract 4
- 108091007433 antigens Proteins 0.000 abstract 4
- 239000012634 fragment Substances 0.000 abstract 4
- 229960004540 secukinumab Drugs 0.000 abstract 4
- 238000000034 method Methods 0.000 abstract 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 abstract 1
- 238000005251 capillar electrophoresis Methods 0.000 abstract 1
- 125000002091 cationic group Chemical group 0.000 abstract 1
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 210000004962 mammalian cell Anatomy 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
- C07K16/244—Interleukins [IL]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
- C07K2317/565—Complementarity determining region [CDR]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
- C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La presente se refiere a métodos para reducir selectivamente CysL97 en una preparación de anticuerpos IL-17 o fragmentos de unión a antígeno de los mismos, ej., una preparación de anticuerpos de secukinumab, que ha sido producida de forma recombinante por células de mamífero, También se proporcionan preparaciones purificadas de anticuerpos IL-17 o fragmentos de unión a antígeno de los mismos producidos por tales métodos, por ejemplo, preparaciones purificadas de secukinumab, en donde el nivel de anticuerpos IL-17 intactos o fragmentos de unión a antígeno de los mismos (por ejemplo, secukinumab) en la preparación es alto, por ejemplo, al menos aproximadamente 90%, medido por electroforesis capilar de dodecil sulfato de sodio (CE-SDS), y en donde el nivel de actividad de anticuerpos IL-17 o fragmentos de unión a antígeno de los mismos (por ejemplo, secukinumab) en la preparación es alto, por ejemplo, al menos aproximadamente 92%, medido por cromatografía de intercambio catiónico (CEX).
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462095361P | 2014-12-22 | 2014-12-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR103172A1 true AR103172A1 (es) | 2017-04-19 |
Family
ID=55135470
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP150104191A AR103172A1 (es) | 2014-12-22 | 2015-12-18 | Reducción selectiva de residuos de cisteina en anticuerpos il-17 |
Country Status (15)
| Country | Link |
|---|---|
| US (3) | US11292833B2 (es) |
| EP (2) | EP3237445A1 (es) |
| JP (4) | JP7021950B2 (es) |
| KR (2) | KR102643328B1 (es) |
| CN (4) | CN114249826B (es) |
| AR (1) | AR103172A1 (es) |
| AU (1) | AU2015370515B2 (es) |
| BR (1) | BR112017010069A2 (es) |
| CA (1) | CA2971541A1 (es) |
| IL (2) | IL251893B2 (es) |
| MX (1) | MX2017008410A (es) |
| PH (1) | PH12017500967A1 (es) |
| RU (2) | RU2719563C2 (es) |
| SG (1) | SG11201703639QA (es) |
| WO (1) | WO2016103146A1 (es) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2017354027C1 (en) * | 2016-11-07 | 2024-04-18 | Seagen Inc. | Distribution of engineered-cysteine caps |
| JP7504025B2 (ja) * | 2017-08-04 | 2024-06-21 | アムジエン・インコーポレーテツド | Ｃｙｓ－ｍａｂのコンジュゲーション方法 |
| CN112805300A (zh) * | 2018-08-15 | 2021-05-14 | 百时美施贵宝公司 | 下游色谱法中依据再氧化的蛋白质片段化控制策略 |
| CN109369806B (zh) * | 2019-01-14 | 2019-04-19 | 迈威(上海)生物科技有限公司 | 苏金单抗制品中半胱氨酸化变异体的去除方法 |
| JP7573261B2 (ja) | 2020-07-28 | 2024-10-25 | 国立大学法人山梨大学 | 抗酸化剤及びその製造方法、医薬組成物及びその製造方法、食品、並びに化粧品 |
| WO2022117773A1 (en) | 2020-12-02 | 2022-06-09 | Fresenius Kabi Deutschland Gmbh | Methods of selectively reducing antibodies |
| KR20230124971A (ko) | 2020-12-22 | 2023-08-28 | 노파르티스 아게 | 세포 배양 중 분비된 재조합 발현 단백질에서 시스테인잔기의 산화 수준을 감소시키는 방법 |
| CN113189184B (zh) * | 2021-04-28 | 2022-09-09 | 浙江大学 | 含有半胱氨酸的毛细管凝胶电泳样品缓冲液 |
| WO2023067626A1 (en) * | 2021-10-20 | 2023-04-27 | Dr. Reddy's Laboratories Limited | A cell culture process to produce an antibody composition |
| WO2024156841A1 (en) * | 2023-01-27 | 2024-08-02 | Fresenius Kabi Deutschland Gmbh | Biopharmaceutical composition |
| EP4406553A1 (en) * | 2023-01-27 | 2024-07-31 | Fresenius Kabi Deutschland GmbH | Stable secukinumab biopharmaceutical composition |
| CN118348098B (zh) * | 2024-03-31 | 2025-01-10 | 珠海联邦生物医药有限公司 | 一种用于毛细管电泳检测的凝胶分离缓冲液 |
Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
| US4766205A (en) | 1985-11-13 | 1988-08-23 | Beatrice Companies, Inc. | Method for isolation of recombinant polypeptides in biologically active forms |
| JP2669859B2 (ja) * | 1987-08-04 | 1997-10-29 | 協和醗酵工業株式会社 | タンパク質の再活性化法 |
| JP3610395B2 (ja) * | 1991-08-26 | 2005-01-12 | 敏一 中村 | 成熟肝実質細胞増殖因子 |
| IL157446A0 (en) | 2001-02-23 | 2004-03-28 | Immunex Corp Immunex Corp | Increased recovery of active proteins |
| AU2002354363A1 (en) * | 2001-12-04 | 2003-06-17 | Mitsubishi Pharma Corporation | Method of activating protein |
| WO2004056872A1 (ja) | 2002-12-20 | 2004-07-08 | Mitsubishi Pharma Corporation | タンパク質のチオール基を保護する方法 |
| GB0417487D0 (en) * | 2004-08-05 | 2004-09-08 | Novartis Ag | Organic compound |
| BRPI0517457A (pt) | 2004-10-22 | 2008-10-07 | Amgen Inc | métodos para reenovelamento de anticorpos recombinantes |
| EP1893632B1 (en) * | 2005-06-17 | 2015-08-12 | Novo Nordisk Health Care AG | Selective reduction and derivatization of engineered factor vii proteins comprising at least one non-native cysteine |
| GB0513852D0 (en) | 2005-07-06 | 2005-08-10 | Celltech R&D Ltd | Biological products |
| EP1984401A2 (en) | 2006-01-31 | 2008-10-29 | Novartis AG | Il-17 antagonistic antibodies fpr treating cancer |
| CA2641588C (en) * | 2006-02-07 | 2014-06-17 | Shire Human Genetic Therapies, Inc. | Stabilized compositions of proteins having a free thiol moiety |
| EP3327026B1 (en) * | 2007-07-09 | 2019-08-21 | Genentech, Inc. | Prevention of disulfide bond reduction during recombinant production of polypeptides |
| ES2613963T3 (es) | 2008-01-18 | 2017-05-29 | Medimmune, Llc | Anticuerpos manipulados con cisteína para conjugación específica de sitio |
| ES2643446T3 (es) * | 2009-06-04 | 2017-11-22 | Novartis Ag | Métodos para la identificación de sitios para conjugación de IgG |
| GB0920127D0 (en) | 2009-11-17 | 2009-12-30 | Ucb Pharma Sa | Antibodies |
| MX356279B (es) | 2010-10-08 | 2018-05-22 | Novartis Ag Star | Métodos para el tratamiento de soriasis usando antagonistas de il-17. |
| AU2014259523B2 (en) * | 2010-11-05 | 2016-02-04 | Novartis Ag | Methods of treating psoriatic arthritis using IL-17 antagonists |
| AR084234A1 (es) * | 2010-12-13 | 2013-05-02 | Novartis Ag | Metodos predictivos y metodos para tratar artritis usando antagonistas de interleuquina 17 (il-17) |
| JP5782279B2 (ja) | 2011-01-20 | 2015-09-24 | 株式会社Ｓｃｒｅｅｎホールディングス | 基板処理方法および基板処理装置 |
| DK2771364T3 (da) | 2011-10-27 | 2019-08-19 | Genmab As | Fremstilling af heterodimere proteiner |
| RU2014125071A (ru) | 2011-11-21 | 2015-12-27 | Новартис Аг | Способы лечения псориатического артрита (psa) с использованием антагонистов il-17 и аллелей ответа psa или отсутствия ответа psa |
| AR103173A1 (es) * | 2014-12-22 | 2017-04-19 | Novarits Ag | Productos farmacéuticos y composiciones líquidas estables de anticuerpos il-17 |
-
2015
- 2015-12-18 AR ARP150104191A patent/AR103172A1/es unknown
- 2015-12-21 RU RU2017126025A patent/RU2719563C2/ru active
- 2015-12-21 EP EP15823803.0A patent/EP3237445A1/en active Pending
- 2015-12-21 AU AU2015370515A patent/AU2015370515B2/en active Active
- 2015-12-21 JP JP2017551390A patent/JP7021950B2/ja active Active
- 2015-12-21 CN CN202210002418.0A patent/CN114249826B/zh active Active
- 2015-12-21 SG SG11201703639QA patent/SG11201703639QA/en unknown
- 2015-12-21 EP EP24170348.7A patent/EP4389143A3/en active Pending
- 2015-12-21 KR KR1020177016755A patent/KR102643328B1/ko active IP Right Grant
- 2015-12-21 IL IL251893A patent/IL251893B2/en unknown
- 2015-12-21 IL IL298761A patent/IL298761A/en unknown
- 2015-12-21 KR KR1020237038206A patent/KR20230159617A/ko not_active Application Discontinuation
- 2015-12-21 CA CA2971541A patent/CA2971541A1/en active Pending
- 2015-12-21 RU RU2020113344A patent/RU2765307C2/ru active
- 2015-12-21 CN CN202111452382.8A patent/CN114010780B/zh active Active
- 2015-12-21 WO PCT/IB2015/059824 patent/WO2016103146A1/en active Application Filing
- 2015-12-21 MX MX2017008410A patent/MX2017008410A/es unknown
- 2015-12-21 CN CN202210002380.7A patent/CN114316044A/zh active Pending
- 2015-12-21 US US15/538,266 patent/US11292833B2/en active Active
- 2015-12-21 BR BR112017010069A patent/BR112017010069A2/pt not_active Application Discontinuation
- 2015-12-21 CN CN201580066406.2A patent/CN107001460B/zh active Active
-
2017
- 2017-05-25 PH PH12017500967A patent/PH12017500967A1/en unknown
-
2022
- 2022-02-04 JP JP2022016666A patent/JP2022062194A/ja active Pending
- 2022-03-01 US US17/683,888 patent/US20220315650A1/en active Pending
-
2023
- 2023-06-01 JP JP2023091199A patent/JP2023110026A/ja active Pending
-
2024
- 2024-08-12 US US18/800,977 patent/US20250034241A1/en active Pending
- 2024-10-16 JP JP2024181366A patent/JP2025011274A/ja active Pending
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