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MX354995B - Metodos y productos para transfeccion de celulas. - Google Patents

Metodos y productos para transfeccion de celulas.

Info

Publication number
MX354995B
MX354995B MX2014006663A MX2014006663A MX354995B MX 354995 B MX354995 B MX 354995B MX 2014006663 A MX2014006663 A MX 2014006663A MX 2014006663 A MX2014006663 A MX 2014006663A MX 354995 B MX354995 B MX 354995B
Authority
MX
Mexico
Prior art keywords
methods
cells
kits
therapeutics
nucleic acids
Prior art date
Application number
MX2014006663A
Other languages
English (en)
Other versions
MX2014006663A (es
Inventor
Angel Matthew
Rohde Christopher
Original Assignee
Factor Bioscience Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=48574831&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX354995(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US13/465,490 external-priority patent/US8497124B2/en
Application filed by Factor Bioscience Inc filed Critical Factor Bioscience Inc
Publication of MX2014006663A publication Critical patent/MX2014006663A/es
Publication of MX354995B publication Critical patent/MX354995B/es

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Abstract

La presente invención se refiere a un método in vitro para reprogramar una célula a un estado menos diferenciado, caracterizado porque comprende; (a) cultivar una célula diferenciada con un medio de reprogramación que contiene albúmina, en donde la albúmina se trata con una resina de intercambio iónico o con carbón; (b) transfectar la célula con una o más moléculas de ARN sintéticas, en donde la una o más moléculas de ARN sintéticas incluye al menos una molécula de ARN que codifica uno o más factores de reprogramación y en donde la transfección resulta en la célula que expresa el uno o más factores de reprogramación; y (c) repetir la etapa (b) al menos dos veces durante cinco días consecutivos para resultar en la célula que se reprograma a un estado menos diferenciado.
MX2014006663A 2011-12-05 2012-12-05 Metodos y productos para transfeccion de celulas. MX354995B (es)

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US201161566948P 2011-12-05 2011-12-05
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US13/465,490 US8497124B2 (en) 2011-12-05 2012-05-07 Methods and products for reprogramming cells to a less differentiated state
US201261664494P 2012-06-26 2012-06-26
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