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MX2016005658A - Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells. - Google Patents

Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells.

Info

Publication number
MX2016005658A
MX2016005658A MX2016005658A MX2016005658A MX2016005658A MX 2016005658 A MX2016005658 A MX 2016005658A MX 2016005658 A MX2016005658 A MX 2016005658A MX 2016005658 A MX2016005658 A MX 2016005658A MX 2016005658 A MX2016005658 A MX 2016005658A
Authority
MX
Mexico
Prior art keywords
differentiation
clustering
suspension
pluripotent stem
cells
Prior art date
Application number
MX2016005658A
Other languages
Spanish (es)
Inventor
Shelley Nelson
Benjamin Fryer
Daina Laniauskas
Marcia Blackmoore
Haiyun Wang
Kostadinka Lilova
Elizabeth Rosocha
Original Assignee
Janssen Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/998,974 external-priority patent/US10377989B2/en
Application filed by Janssen Biotech Inc filed Critical Janssen Biotech Inc
Publication of MX2016005658A publication Critical patent/MX2016005658A/en

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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/18—Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
    • A—HUMAN NECESSITIES
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Abstract

The present invention provides methods of preparing aggregated pluripotent stem cell clusters for differentiation. Specifically, the invention discloses methods of differentiating pluripotent cells into beta cell, cardiac cell and neuronal cell lineages using suspension clustering. The methods involve preparing the aggregated cell clusters followed by differentiation of these clusters.
MX2016005658A 2013-11-01 2014-06-17 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells. MX2016005658A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361962158P 2013-11-01 2013-11-01
US13/998,974 US10377989B2 (en) 2012-12-31 2013-12-30 Methods for suspension cultures of human pluripotent stem cells
PCT/US2014/042796 WO2015065537A1 (en) 2013-11-01 2014-06-17 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

Publications (1)

Publication Number Publication Date
MX2016005658A true MX2016005658A (en) 2017-01-05

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MX2016005657A MX2016005657A (en) 2013-11-01 2014-05-21 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells.
MX2016005658A MX2016005658A (en) 2013-11-01 2014-06-17 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells.

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MX2016005657A MX2016005657A (en) 2013-11-01 2014-05-21 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells.

Country Status (12)

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EP (2) EP3063268A4 (en)
JP (2) JP2016534731A (en)
KR (4) KR20180128529A (en)
CN (2) CN105793413A (en)
AU (4) AU2014342995C1 (en)
BR (1) BR112016009393A8 (en)
CA (2) CA2928639A1 (en)
MX (2) MX2016005657A (en)
PH (2) PH12016500783A1 (en)
RU (2) RU2689710C2 (en)
SG (2) SG11201603045VA (en)
WO (2) WO2015065524A2 (en)

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CN114729323B (en) * 2019-11-22 2024-09-17 诺和诺德股份有限公司 Rotationally aggregated neural microsphere and application thereof
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CN116218766A (en) * 2023-04-21 2023-06-06 北京意胜生物科技有限公司 Method for preparing pancreatic precursor cells, culture medium and application of pancreatic precursor cells

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PH12016500782A1 (en) 2016-06-13
RU2016121409A3 (en) 2018-03-29
SG11201603047PA (en) 2016-05-30
KR20180130001A (en) 2018-12-05
JP2017500013A (en) 2017-01-05
PH12016500783A1 (en) 2016-06-13
CA2928741A1 (en) 2015-05-07
AU2014342995C1 (en) 2018-08-09
EP3063268A2 (en) 2016-09-07
RU2016121404A3 (en) 2018-03-29
KR20160079071A (en) 2016-07-05
WO2015065524A2 (en) 2015-05-07
KR20160079072A (en) 2016-07-05
AU2014342995B2 (en) 2018-04-26
AU2014343007A1 (en) 2016-05-05
WO2015065524A3 (en) 2015-08-13
KR20180128529A (en) 2018-12-03
CN105793413A (en) 2016-07-20
MX2016005657A (en) 2017-01-05
AU2014343007C1 (en) 2018-08-09
AU2014342995A1 (en) 2016-05-05
EP3063269A1 (en) 2016-09-07
JP2016534731A (en) 2016-11-10
SG11201603045VA (en) 2016-05-30
RU2016121404A (en) 2017-12-04
CN105683362A (en) 2016-06-15
AU2018208707A1 (en) 2018-08-16
AU2014343007B2 (en) 2018-04-26
EP3063269A4 (en) 2017-07-12
BR112016009393A8 (en) 2020-03-24
RU2689710C2 (en) 2019-05-29
WO2015065537A1 (en) 2015-05-07
EP3063268A4 (en) 2017-11-29
RU2016121409A (en) 2017-12-04
AU2018208717A1 (en) 2018-08-16

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