Nothing Special   »   [go: up one dir, main page]

Vaughan et al. - Google Patents

Purification of Plasmid DNA Using Corning® Glass Fiber Filter Plates

Vaughan et al.

View PDF
Document ID
2000514793912121524
Author
Vaughan L
Jiang H
Belkowski L
Portsmouth N

External Links

Snippet

1. Inoculate 1 mL of Luria Broth (LB) plus appropriate antibiotic with a single colony of E. coli into each well of a Corning® 2 mL block (Cat. No. 3960) and grow for 16 h with shaking (150 rpm). 2. Harvest the cells by centrifugation (1500 xg) for 5 minutes and carefully decant the …
Continue reading at nsk.dia-m.ru (PDF) (other versions)

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving viable micro-organisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact micro-organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification

Similar Documents

Publication Publication Date Title
EP1088064B1 (en) Rapid and simple process for isolation of circular nucleic acids
US20080300397A1 (en) Modified spin column for simple and rapid plasmid dna extraction
US7867751B2 (en) Plasmid DNA isolation
US6864100B1 (en) Automated protein purification the multiwell format by vacuum filtration
KR100785010B1 (en) Method and apparatus for the purification of nucleic acids on hydrophilic surface of solid support using hydrogen bonding
US6355792B1 (en) Method for isolating and purifying nucleic acids
US20090246859A1 (en) Methods and Compositions for Purification of Nucleic Acid from a Host Cell
US20080299621A1 (en) Miniprep system for simple and rapid plasmid dna extraction
AU778440B2 (en) Sample processing device
JPH1192494A (en) Isolation of dna
EP1173623A1 (en) Solid medium and process for the storage and rapid purification of nucleic acid
EP2137309B1 (en) Methods for the separation of biological molecules using sulfolane
US20090227011A1 (en) Methods of purifying plasmid dna
WO1990014416A1 (en) Improved composition for isolating and purifying nucleic acid and improved method using same
CA2684455A1 (en) Method and apparatus for processing substances in a single container
US5106966A (en) Use of gel polymer for dna extraction with organic solvents
Vaughan et al. Purification of Plasmid DNA Using Corning® Glass Fiber Filter Plates
TW416867B (en) Separation apparatus, separation method, nucleic acid separation purification method, nucleic acid separation purification kit
JP3987342B2 (en) Chromatographic material and method of use
US5175271A (en) Use of silica gel for DNA extraction with organic solvents
US20030228600A1 (en) DNA isolation method and kit
Olson et al. Simultaneous preparation of up to 768 single-stranded DNAs for use as templates in DNA sequencing