Shim et al., 2009 - Google Patents
Simultaneous determination of gene expression and enzymatic activity in individual bacterial cells in microdroplet compartmentsShim et al., 2009
View PDF- Document ID
- 16378292128884900774
- Author
- Shim J
- Olguin L
- Whyte G
- Scott D
- Babtie A
- Abell C
- Huck W
- Hollfelder F
- Publication year
- Publication venue
- Journal of the American Chemical Society
External Links
Snippet
A microfluidic device capable of storing picoliter droplets containing single bacteria at constant volumes has been fabricated in PDMS. Once captured in droplets that remain static in the device, bacteria express both a red fluorescent protein (mRFP1) and the enzyme …
- 230000014509 gene expression 0 title abstract description 69
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated micro-fluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Shim et al. | Simultaneous determination of gene expression and enzymatic activity in individual bacterial cells in microdroplet compartments | |
Ding et al. | Recent advances in droplet microfluidics | |
Kim et al. | Single-cell RT-PCR in microfluidic droplets with integrated chemical lysis | |
Zhu et al. | Hydrogel droplet microfluidics for high-throughput single molecule/cell analysis | |
Vallejo et al. | Fluorescence-activated droplet sorting for single-cell directed evolution | |
Huebner et al. | Development of quantitative cell-based enzyme assays in microdroplets | |
Zinchenko et al. | One in a million: flow cytometric sorting of single cell-lysate assays in monodisperse picolitre double emulsion droplets for directed evolution | |
Najah et al. | Teaching single-cell digital analysis using droplet-based microfluidics | |
Mazutis et al. | Droplet-based microfluidic systems for high-throughput single DNA molecule isothermal amplification and analysis | |
Chen et al. | Chemical transfection of cells in picoliter aqueous droplets in fluorocarbon oil | |
Wheeler et al. | Microfluidic device for single-cell analysis | |
Zhan et al. | Electroporation of cells in microfluidic droplets | |
Konry et al. | Innovative tools and technology for analysis of single cells and cell–cell interaction | |
Schneider et al. | The potential impact of droplet microfluidics in biology | |
Clausell-Tormos et al. | Droplet-based microfluidic platforms for the encapsulation and screening of mammalian cells and multicellular organisms | |
JP6169111B2 (en) | Methods, systems, and devices for capturing and processing multiple single cells using microfluidics | |
Brower et al. | Double emulsion picoreactors for high-throughput single-cell encapsulation and phenotyping via FACS | |
Kou et al. | Microfluidics and microbial engineering | |
Oliveira et al. | Microfluidic tools toward industrial biotechnology | |
US8906669B2 (en) | Microfluidic multiplexed cellular and molecular analysis device and method | |
Hess et al. | High-throughput, quantitative enzyme kinetic analysis in microdroplets using stroboscopic epifluorescence imaging | |
Schneider et al. | Self-digitization of samples into a high-density microfluidic bottom-well array | |
CN102449164A (en) | Device and method for producing a replicate or derivative from an array of molecules, and applications thereof | |
Price et al. | h ν SABR: Photochemical Dose–Response Bead Screening in Droplets | |
Ven et al. | Target confinement in small reaction volumes using microfluidic technologies: a smart approach for single-entity detection and analysis |