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Tsai et al., 2014 - Google Patents

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

Tsai et al., 2014

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Document ID
10441359747172447890
Author
Tsai S
Wyvekens N
Khayter C
Foden J
Thapar V
Reyon D
Goodwin M
Aryee M
Joung J
Publication year
Publication venue
Nature biotechnology

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Monomeric CRISPR-Cas9 nucleases are widely used for targeted genome editing but can induce unwanted off-target mutations with high frequencies. Here we describe dimeric RNA- guided FokI nucleases (RFNs) that can recognize extended sequences and edit …
Continue reading at www.ncbi.nlm.nih.gov (HTML) (other versions)

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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