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Du et al., 2004 - Google Patents

Validating siRNA using a reporter made from synthetic DNA oligonucleotides

Du et al., 2004

Document ID
4343688779967593549
Author
Du Q
Thonberg H
Zhang H
Wahlestedt C
Liang Z
Publication year
Publication venue
Biochemical and biophysical research communications

External Links

Snippet

Only a small fraction of all siRNAs are effective in silencing their target genes, and siRNA efficacy can only be determined experimentally. Previously described reporter-based siRNA validation methods all rely on the availability of physical cDNA clones, and this limits the …
Continue reading at www.sciencedirect.com (other versions)

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    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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