The reproductive ecology of four sympatric Drosophila virths group species (Diptera Drosophilidae... more The reproductive ecology of four sympatric Drosophila virths group species (Diptera Drosophilidae) was studied in the field to trace the factors maintaining species isolation There was a high probability of meeting a heterospecific individual belonging to the same species group on the basis of adult spatial occurrence Direct field observations revealed that the males court also females of the foreign species but interspecific courtships do not lead to mating The main mating period among these species lasted only several weeks and the timing of mating periods differed markedlv in the three most common species Reproductive character displacement between species may explain the observed differences in the timing of the mating periods but they could have arisen in the context of other selective pressures than the prevention of interspecific courtships and matings
Additional file 8: Table S8. NEWHYBRIDS and STRUCTURE results for wolves living in two animal par... more Additional file 8: Table S8. NEWHYBRIDS and STRUCTURE results for wolves living in two animal parks in Germany.
Additional file 12: Table S12. Description of SNPs and primer sequences used in this study. Allel... more Additional file 12: Table S12. Description of SNPs and primer sequences used in this study. Allele frequencies for the 93 SNPs are also reported. The first tab contains 96 SNPs that were included in the final SNP panel. Three SNPs that were removed before the analysis are indicated. The remaining SNPs that were tested but not included in the final panel and their corresponding primer sequences are in the second tab.
Additional file 17: Figure S5. Delta K values for 1 ≤ K ≤ 8 when analyzed whole dataset with STRU... more Additional file 17: Figure S5. Delta K values for 1 ≤ K ≤ 8 when analyzed whole dataset with STRUCTURE. K = 2 had highest value.
Additional file 5: Table S5. The first tab contains names, locations and sampling dates for the s... more Additional file 5: Table S5. The first tab contains names, locations and sampling dates for the samples that were genotyped in this study. Column named "Replicates" in the first tab shows how many times the same sample was genotyped, and the number in the parenthesis shows how many times the same individual was genotyped. Samples that were not included in the analysis are indicated. The second tab contains names, locations and sampling dates for the samples from the wolves from Italy and the Iberian Peninsula that were genotyped with the CanineHD Whole-Genome BeadChip (Illumina). The third tab contains sample names and breeds for the dog samples that were genotyped with the Illumina CanineHD chip. In each tab, there are results from the STRUCTURE and NEWHYBRIDS runs, with all possible prior combinations, for all the individuals included in the runs. When the result differed between the runs, several results were included. If the analysis was been done using a consensus gen...
Biplots of the first two PCs in supertype clusters of DLA-DRB1 (Figure S5) and DLA-DQB1 loci (Fig... more Biplots of the first two PCs in supertype clusters of DLA-DRB1 (Figure S5) and DLA-DQB1 loci (Figure S6). The biplots were done using the ggbiplot function in R. Ellipses show Normal contour lines with 68% probability for each cluster. (ZIP 20Â kb)
Result table for segregation of heterozygous and homozygous offspring in different mating cross t... more Result table for segregation of heterozygous and homozygous offspring in different mating cross types for the MHC loci, the three-locus haplotype and the MHC supertype levels, heterozygous mothers combined and the numbers of offspring that inherited maternal shared or paternal non-shared allele from their father. (XLSX 12Â kb)
The reproductive ecology of four sympatric Drosophila virths group species (Diptera Drosophilidae... more The reproductive ecology of four sympatric Drosophila virths group species (Diptera Drosophilidae) was studied in the field to trace the factors maintaining species isolation There was a high probability of meeting a heterospecific individual belonging to the same species group on the basis of adult spatial occurrence Direct field observations revealed that the males court also females of the foreign species but interspecific courtships do not lead to mating The main mating period among these species lasted only several weeks and the timing of mating periods differed markedlv in the three most common species Reproductive character displacement between species may explain the observed differences in the timing of the mating periods but they could have arisen in the context of other selective pressures than the prevention of interspecific courtships and matings
Additional file 8: Table S8. NEWHYBRIDS and STRUCTURE results for wolves living in two animal par... more Additional file 8: Table S8. NEWHYBRIDS and STRUCTURE results for wolves living in two animal parks in Germany.
Additional file 12: Table S12. Description of SNPs and primer sequences used in this study. Allel... more Additional file 12: Table S12. Description of SNPs and primer sequences used in this study. Allele frequencies for the 93 SNPs are also reported. The first tab contains 96 SNPs that were included in the final SNP panel. Three SNPs that were removed before the analysis are indicated. The remaining SNPs that were tested but not included in the final panel and their corresponding primer sequences are in the second tab.
Additional file 17: Figure S5. Delta K values for 1 ≤ K ≤ 8 when analyzed whole dataset with STRU... more Additional file 17: Figure S5. Delta K values for 1 ≤ K ≤ 8 when analyzed whole dataset with STRUCTURE. K = 2 had highest value.
Additional file 5: Table S5. The first tab contains names, locations and sampling dates for the s... more Additional file 5: Table S5. The first tab contains names, locations and sampling dates for the samples that were genotyped in this study. Column named "Replicates" in the first tab shows how many times the same sample was genotyped, and the number in the parenthesis shows how many times the same individual was genotyped. Samples that were not included in the analysis are indicated. The second tab contains names, locations and sampling dates for the samples from the wolves from Italy and the Iberian Peninsula that were genotyped with the CanineHD Whole-Genome BeadChip (Illumina). The third tab contains sample names and breeds for the dog samples that were genotyped with the Illumina CanineHD chip. In each tab, there are results from the STRUCTURE and NEWHYBRIDS runs, with all possible prior combinations, for all the individuals included in the runs. When the result differed between the runs, several results were included. If the analysis was been done using a consensus gen...
Biplots of the first two PCs in supertype clusters of DLA-DRB1 (Figure S5) and DLA-DQB1 loci (Fig... more Biplots of the first two PCs in supertype clusters of DLA-DRB1 (Figure S5) and DLA-DQB1 loci (Figure S6). The biplots were done using the ggbiplot function in R. Ellipses show Normal contour lines with 68% probability for each cluster. (ZIP 20Â kb)
Result table for segregation of heterozygous and homozygous offspring in different mating cross t... more Result table for segregation of heterozygous and homozygous offspring in different mating cross types for the MHC loci, the three-locus haplotype and the MHC supertype levels, heterozygous mothers combined and the numbers of offspring that inherited maternal shared or paternal non-shared allele from their father. (XLSX 12Â kb)
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