Papers by ahmad faris mohd adnan
Sains Malaysiana, 2021
A chitinase-producing bacteria was locally isolated from rhinoceros beetle (Oryctes rhinoceros) l... more A chitinase-producing bacteria was locally isolated from rhinoceros beetle (Oryctes rhinoceros) larvae gut discovered in mushroom compost. A strain was selected from the source and labeled as RBLG1. It was identified using 16s rDNAsequencing and has strong homology to Bacillus cereus. Chitinase produced from RBLG1 was purified using ammonium sulfate precipitation, ion exchange chromatography, and gel filtration. The purified chitinase from RBLG1 shows the homogeneity 1.9 fold after the gel filtration process. The purified chitinase was identified and analyzed using SDS-PAGE and the molecular mass from the strain was approximately 40 kDa. Further assays were performed to study the effect of temperature, pH, and metal ion on the purified enzyme. The purified enzyme shows highest chitinase activity at pH 4 and 60 °C. For metal ion test, chitinase from RBLG1 was unaffected when treated by various ions. Kinetic performance of chitinase using Michaelis-Menten equation shows Km and Vmax va...
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Materials Research Innovations, Aug 2011
Anthocyanins are an important group of natural pigments that are responsible for many colours in ... more Anthocyanins are an important group of natural pigments that are responsible for many colours in plants. The variation in colour, depending on the pH, makes them a unique source of natural colourant. In this study, pigments from the fruits of Ixora siamensis and the fruit pulps of Melastoma malabathricum were extracted using trifluoroacetic acid?methanol solution. Spectral measurements (380?780 nm) were performed using visible spectroscopy with colour analysis software at different pHs (initial extracts were 1, 5, 7, 9 and 11). The colours of the solutions were expressed as colourimetric coordinates in the Commission Internationale de l’Eclairage (CIE) laboratory scale using L* (lightness), C* (chroma), H° (hue angle notation Hab), a*/?a* (redness and greenness) and b*/?b* (blueness and yellowness) for the D65/2°CIE Illuminant/Observer condition. In this work, the colour parameters were observed for natural colourant with and without blending with polyvinyl alcohol for both species (Ixora siamensis and Melastoma malabathricum). The relationships between the colour parameters (colourimetric indexes and CIELab variables) with pH variation and species dependence were discussed in this paper.
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Pigment & Resin Technology, Jun 26, 2012
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Pigment & Resin Technology, May 17, 2013
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African Journal of Microbiology Research, Mar 18, 2011
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Polymer Degradation and Stability, Jun 30, 2008
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Bioresource Technology, 2007
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Papers by ahmad faris mohd adnan