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Effects of MeJA and SA elicitation on secondary metabolic activity, antioxidant content and callogenesis in Phyllanthus pulcher

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Abstract

To develop in vitro callus induction of Phyllanthus pulcher and investigate secondary metabolite activities, methyl jasmonate (MeJA) and salicylic acid (SA) as two abiotic elicitors were evaluated. Growth and secondary metabolite production of callus from P. pulcher grown in Murashige and Skoog medium, supplemented with 1 mg L−1 2, 4-dinitrophenylhydrazine (2, 4-D) and SA with a lower concentration (≤5 mg L−1) showed a significant effect on callus fresh weight, while the dry weight was not affected significantly by higher concentrations. However, in secondary metabolite content, including total flavonoid and phenolic contents and antioxidant activities, significant differences among different SA concentrations were observed. The results revealed that the high concentration of MeJA (>10 mM) inhibits the callus growth, and 1 mM of MeJA resulted in the highest yield for total flavonoid and phenolic contents and antioxidant activity. A very strong positive relationship (0.969) among TFC, TPC, and antioxidant activity was revealed.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxy acetic acid

AA:

Antioxidant activity

ANCOVA:

Analysis of covariance

ANOVA:

Analysis of variance

DMRT:

Duncan’s multiple range test

DW:

Dry weight

FW:

Fresh weight

MeJA:

Methyl jasmonate

MS:

Murashige and Skoog (1962) medium

PGR:

Plant growth regulators

RCBD:

Randomized complete block design

SA:

Salicylic acid

TFC:

Total flavonoid content

TPC:

Total phenolic content

References

  • Ali MB, Hahn E-J, Paek K-Y (2007) Methyl jasmonate and salicylic acid induced oxidative stress and accumulation of phenolics in Panax ginseng bioreactor root suspension cultures. Molecules 12:607–621

    Article  CAS  PubMed  Google Scholar 

  • Bagalkotkar G (2007) Isolation and characterisation of compounds from ‘Naga Buana’ (Phyllanthus pulcher) and ‘Similit Matinggi’ (Casearia capitellata) and their cytotoxic effects on cancer cell lines. In: Faculty of medicine and health science, vol PhD. vol 7188. Universiti Putra Malaysia, Serdang

  • Basse CW, Fath A, Boller T (1993) High affinity binding of a glycopeptide elicitor to tomato cells and microsomal membranes and displacement by specific glycan suppressors. J Biol Chem 268:14724–14731

    CAS  PubMed  Google Scholar 

  • Colombo R et al (2009) Validated HPLC method for the standardization of Phyllanthus niruri (herb and commercial extracts) using corilagin as a phytochemical marker. Biomed Chromatogr 23:573–580. doi:10.1002/bmc.1155

    Article  CAS  PubMed  Google Scholar 

  • Cosio EG, Frey T, Verduyn R, van Boom J, Ebel J (1990) High-affinity binding of a synthetic heptaglucoside and fungal glucan phytoalexin elicitors to soybean membranes. FEBS Lett 271:223–226

    Article  CAS  PubMed  Google Scholar 

  • Dixon RA, Dey PM, Murphy DL, Whitehead IM (1981) Dose responses for Colletotrichum lindemuthianum elicitor-mediated enzyme induction in French bean cell suspension cultures. Planta 151:272–280. doi:10.1007/bf00395180

    Article  CAS  PubMed  Google Scholar 

  • Dörnenburg H, Knorr D (1995) Strategies for the improvement of secondary metabolite production in plant cell cultures. Enzym Microb Technol 17:674–684. doi:10.1016/0141-0229(94)00108-4

    Article  Google Scholar 

  • Duangporn P, Siripong P (2009) Effect of auxin and cytokinin on Phyllanthusol A production by callus cultures of Phyllanthus acidus Skeels. American-Eurasian J Agric Environ Sci 5:258–263

    CAS  Google Scholar 

  • Fang Y, Smith M, Pepin M-F (1999) Effects of exogenous methyl jasmonate in elicited anthocyanin-producing cell cultures of ohelo (Vaccinium phalae). In Vitro Cell Dev Biol Plant 35:106–113

    Article  CAS  Google Scholar 

  • Farzinebrahimi R, Taha RM, Kamaludin R, Syafawati Yaacob J (2014) The effect of various media and hormones via suspension culture on secondary metabolic activities of (Cape Jasmine) Gardenia jasminoides Ellis. Sci World J 2014. doi:10.1155/2014/407284

    Google Scholar 

  • Fernand VE (2003) Initial characterization of crude extracts from Phyllanthus amarus Schum. and Thonn. and Quassia amara L. using normal phase thin layer chromatography. Louisiana State University, Baton Rouge

    Google Scholar 

  • Galal A (2012) Improving effect of salicylic acid on the multipurpose tree Ziziphus spina-christi (L.) wild tissue culture. Am J Plant Sci 3:947–952

    Article  CAS  Google Scholar 

  • Heinstein PF (1985) Future approaches to the formation of secondary natural products in plant cell suspension cultures. J Nat Prod 48:1–9. doi:10.1021/np50037a001

    Article  CAS  Google Scholar 

  • Henstrand JM, McCue KF, Brink K, Handa AK, Herrmann KM, Conn EE (1992) Light and fungal elicitor induce 3-Deoxy-d-arabino-heptulosonate 7-phosphate synthase mRNA in suspension cultured cells of parsley (Petroselinum crispum L.). Plant Physiol 98:761–763

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Hodzic Z, Pasalic H, Memisevic A, Srabovic M, Saletovic M, Poljakovic M (2009) The influence of total phenols content on antioxidant capacity in the whole grain extracts. Eur J Sci Res 28:471–477

    Google Scholar 

  • Ismail M, Bagalkotkar G, Iqbal S, Adamu HA (2012) Anticancer properties and phenolic contents of sequentially prepared extracts from different parts of selected medicinal plants indigenous to Malaysia. Molecules 17:5745–5756

    Article  CAS  PubMed  Google Scholar 

  • Ketchum REB, Gibson DM, Croteau RB, Shuler ML (1999) The kinetics of taxoid accumulation in cell suspension cultures of Taxus following elicitation with methyl jasmonate. Biotechnol Bioeng 62:97–105

    Article  CAS  PubMed  Google Scholar 

  • Kim D-O, Jeong SW, Lee CY (2003) Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem 81:321–326. doi:10.1016/S0308-8146(02)00423-5

    Article  CAS  Google Scholar 

  • Lim YY, Murtijaya J (2007) Antioxidant properties of Phyllanthus amarus extracts as affected by different drying methods. LWT-Food Sci Technol 40:1664–1669. doi:10.1016/j.lwt.2006.12.013

    Article  CAS  Google Scholar 

  • Lloyd G, Mc Cown B (1980) Commercially feasible micropropagation of mountain laurel Kalmia latifolia, by use of shoot tip culture. Comb Proc Int Plant Propag Soc 30:421–426

    Google Scholar 

  • Loh SI (2007) Evaluation hepatoprotektif chloroform extract Phyllanthus pulcher wall. mϋll ex. arg. and exclusions extract from flavonoids metanolnya removal based on free radical activity. University Science Malaysia (USM), Gelugor

    Google Scholar 

  • Masoumian M, Arbakariya A, Syahida A, Maziah M (2011) Flavonoids production in Hydrocotyle bonariensis callus tissues. J Med Plants Res 5:1564–1574

    CAS  Google Scholar 

  • Mehmetoglu Ü, Curtis WR (1997) Effects of abiotic inducers on sesquiterpene synthesis in hairy root and cell-suspension cultures of Hyoscyomus muticus. Appl Biochem Biotechnol 67:71–77

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco cultures. Physiol Plant J 15:473–497

    Article  CAS  Google Scholar 

  • Nürnberger T, Nennstiel D, Jabs T, Sacks WR, Hahlbrock K, Scheel D (1994) High affinity binding of a fungal oligopeptide elicitor to parsley plasma membranes triggers multiple defense responses. Cell 78:449–460

    Article  PubMed  Google Scholar 

  • Prakash G, Srivastava AK (2008) Statistical elicitor optimization studies for the enhancement of azadirachtin production in bioreactor Azadirachta indica cell. Biochem Eng J 40:218–226. doi:10.1016/j.bej.2007.12.017

    Article  CAS  Google Scholar 

  • Rivas-San Vicente M, Plasencia J (2011) Salicylic acid beyond defence: its role in plant growth and development. J Exp Bot 62:3321–3338

    Article  CAS  PubMed  Google Scholar 

  • Sakhanokho HF, Kelley RY (2009) Influence of salicylic acid on in vitro propagation and salt tolerance in Hibiscus acetosella and Hibiscus moscheutos (cv ‘Luna Red’). Afr J Biotechnol 8:1474–1481

    CAS  Google Scholar 

  • Shilpa K, Selvakkumar C, Senthil A, Lakshmi B (2010a) In vitro root culture of Ocimum sanctum L. and evaluation of its free radical scavenging activity. Plant Cell Tissue Organ Culture 101:105–109

    Article  CAS  Google Scholar 

  • Shilpa K, Varun K, Lakshmi BS (2010b) An alternate method of natural drug production: eliciting secondary metabolite production using plant cell culture. J Plant Sci 5:222–247. doi:10.3923/jps.2010.222.247

    Article  Google Scholar 

  • Smith M, Kobayashi H, Gawienowski M, Briskin D (2002) An in vitro approach to investigate medicinal chemical synthesis by three herbal plants plant cell. Tissue Organ Culture 70:105–111. doi:10.1023/a:1016081913719

    Article  CAS  Google Scholar 

  • Sudha G, Ravishankar GA (2003) Elicitation of anthocyanin production in callus cultures of Daucus carota and the involvement of methyl jasmonate and salicylic acid. Acta Physiol Plant 25:249–256

    Article  CAS  Google Scholar 

  • Thanh N, Murthy H, Yu K, Hahn E, Paek K (2005) Methyl jasmonate elicitation enhanced synthesis of ginsenoside by cell suspension cultures of Panax ginseng in 5-l balloon type bubble bioreactors. Appl Microbiol Biotechnol 67:197–201

    Article  CAS  PubMed  Google Scholar 

  • Theerakulpisut P, Kanawapee N, Maensiri D, Bunnag S, Chantaranothai P (2008) Development of species-specific SCAR markers for identification of three medicinal species of Phyllanthus. J Syst Evol 46:614–621

    Google Scholar 

  • Wickremesinhe EM, Arteea R (1993) Taxus callus cultures: initiation, growth optimization, characterization and taxol production. Plant Cell Tissue Organ Culture 35:181–193. doi:10.1007/bf00032968

    Article  CAS  Google Scholar 

  • Wongwicha W, Tanaka H, Shoyama Y, Putalun W (2011) Methyl jasmonate elicitation enhances glycyrrhizin production in Glycyrrhiza inflata hairy roots cultures Zeitschrift fur Naturforschung C. J Biosci 66:423

    CAS  Google Scholar 

  • Wu J, Lin L (2002) Elicitor-like effects of low-energy ultrasound on plant (Panax ginseng) cells: induction of plant defense responses and secondary metabolite production. Appl Microbiol Biotechnol 59:51–57

    Article  CAS  PubMed  Google Scholar 

  • Xu YW, Zhao D, Lv SS, Yang WT, Chen JW, Wu W (2011) Salicylic acid-induced physiological responses and monoterpene accumulation in Houttuynia cordata Thunb. J Med Plants Res 5:4832–4837

    CAS  Google Scholar 

  • Zabala MA, Angarita M, Restrepo JM, Caicedo LA, Perea M (2010) Elicitation with methyl-jasmonate stimulates peruvoside production in cell suspension cultures of Thevetia peruviana. In Vitro Cell Dev Biol Plant 46:233–238

    Article  CAS  Google Scholar 

  • Zhishen J, Mengcheng T, Jianming W (1999) The determination of flavonoid contents in Mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555–559. doi:10.1016/S0308-8146(98)00102-2

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by University Putra Malaysia. We also thank the University of Malaya for supporting and helping to publish this research.

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Authors and Affiliations

  1. Faculty of Agriculture, Islamic Azad University, Roudehen Branch, Rudehen, Tehran, Iran

    Mahmoud Danaee

  2. Department of Agricultural Technology, Faculty of Agriculture, University Putra Malaysia, Serdang, Selangor, Malaysia

    Mihdzar Abdul Kadir

  3. Department of Crop Science, Faculty of Agriculture, University Putra Malaysia, Serdang, Selangor, Malaysia

    Uma Rani Sinniah

  4. Department of Bioprocess, Faculty of Biotechnology, University Putra Malaysia, Serdang, Selangor, Malaysia

    Rosfarizan Mohamad

  5. Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia

    Reza Farzinebrahimi & Rosna Mat Taha

  6. UMCAS, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia

    Mahmoud Danaee

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  1. Mahmoud Danaee
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  2. Reza Farzinebrahimi
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  4. Uma Rani Sinniah
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Correspondence to Mahmoud Danaee.

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Danaee, M., Farzinebrahimi, R., Kadir, M.A. et al. Effects of MeJA and SA elicitation on secondary metabolic activity, antioxidant content and callogenesis in Phyllanthus pulcher . Braz. J. Bot 38, 265–272 (2015). https://doi.org/10.1007/s40415-015-0140-3

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