Abstract
In this study, we assessed the anticancer activity and bacterial enzyme inhibition of Bifidobacterium adolescentis SPM0212. B. adolescentis SPM0212 inhibited the proliferation of three human colon cancer cell lines: HT-29, SW 480, and Caco-2. SPM0212 also dose-dependently inhibited TNF-á production and changes in cellular morphology. B. adolescentis SPM0212 inhibited harmful fecal enzymes, including â-glucuronidase, â-glucosidase, tryptophanase, and urease. Thus, B. adolescentis SPM0212 exerts an anticancer effect and inhibits harmful fecal enzymes.
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Ashkenazi, A. and Dixit, V. M., Death receptors: signaling and modulation. Science, 287, 1305–1308 (1998).
Choi, S. S., Kang, B. Y., Chung, M. J., Kim, S. D., Park, S. H., Kim, J. S., Kang, C. Y., and Ha, N. J., Safety assessment of potential lactic acid bacteria Bifidobacterium longum SPM1205 isolated from healthy Koreans. J. Microbiol., 43, 493–498 (2005).
de la Chapelle, A., Genetic predisposition to colorectal cancer. Nat. Rev. Cancer, 4, 769–780 (2004).
Fernandes, C. F. and Shahani, K. M., Anticarcinogenic and immunological properties of dietary lactobacilli. J. Food Prot., 53, 704–710 (1990).
Goldin, B. R. and Gorbach, S. L., The relationship between diet and rat fecal bacterial enzymes. J. Natl. Cancer Inst, 57, 371–375 (1976).
Gomez, E., Melar, M. M., Silva, G. P., Portoles, A., and Gil, I., Exocellular products from Bifidobacterium adolescentis as immunomodifiers in the lymphoproliferative responses of mouse splenocytes. FEMS. Microbiol. Lett., 56, 47–52 (1998).
Greenlee, R. T., Hill-Harmon, M. B., Murray, T., and Thun, M., Cancer statistics. CA Cancer J. Clin, 51, 144 (2001).
Kado-Oka, Y., Fujiwara, S., and Hirota, T., Effects of bifidobacteria cells on mitogenic response of splenocytes and several functions of phagocytes. Milchwissenshaft, 46, 626–630 (1991).
Kim, D. H., Kang, H. J., Kim, S. W., and Kobayashi, K., pH-inducible β-glucuronidase and β-glucosidase of intestinal bacteria. Chem. Pharm. Bull., 40, 1967–1969 (1992).
Kim, D. H., Lee, J. H., Bae, E. A., and Han, M. J., Induction and inhibition of indole of intestinal bacteria. Arch. Pharm. Res., 18, 351–355 (1995).
Lee, J., Ametani, A., Enomoto, A., Sato, Y., Motoshima, H., Ike, R., and Kaminogawa, S., Screening for the immunopotentiating activity of food microorganisms and enhancement of the immune response by Bifidobacterium adolescentis M101-4, Biosci. Biotech. Biochem., 57, 2127–2132 (1993).
Maclennan, R. and Jensen, O. M., Dietary fibre, transit time, fecal bacteria, steroids and colon cancer in two Scandiavian populations. Lancet, 30, 207–211 (1977).
Malhotra, S. L., Dietary factors in a study of colon cancer from Cancer Registry, with special reference to the role of saliva, milk and fermented milk products and vegetable fibre. Med. Hypotheses, 3, 122–126 (1977).
Manjunath, N. and Ranganathan, B., A cytotoxic substance produced by a wild culture of Lactobacillus casei D-34 against tumor cells. Indian J. Exp. Biol., 27, 141–145 (1989).
Natoli, G., Costanzo, A., Guido, F., Moretti, F., and Lovreto, M., Apoptotic, non-apoptotic and anti-apoptotic pathways of TNF signaling. Biochem. Pharmacol., 56, 915–920 (1998).
Oda, M., Hasegawa, H., Komatsu, S., Kambe, M., and Tsuchiya, F., Anti-tumor polysaccharide from Lactobacillus sp. Agric. Biol. Chem., 47, 1623–1625 (1983).
Pisani, P., Parkin, D. M., and Ferlay, J., Estimates of the worldwide mortality from eighteen major cancers in 1985. Int. J. Cancer, 54, 594–606 (1993).
Rafter, J. J., The role of lactic acid bacteria in colon cancer prevention. Scand. J. Gastroenterol., 30, 497–502 (1995).
Reddy, B. S., Nutritional factors and colon cancer. Crit. Rev. Fd. Sci. Nutr., 35, 175–190 (1995).
Reddy, B. S. and Wynder, E., Metabolic epidemiology of colorectal cancer: fecal bile acids and neutral steroids in colon cancer patients with adenomatous polyps. Cancer, 39, 2533–2539 (1977).
Scardovi, V., Genus Bifidobacterium, p. 1418–1434. In N.R. Krieg and J. G. Holt (ed.), Bergey’s Manual of Systemic Bacteriology, vol. 2, Williams & Willikins, MD (1986).
Sekine, K., Ohta, J., Onishi, M., Tatsuki, T., Shimokawa, Y., Toida, T., Kawashima, T., and Hashimoto, Y., Analysis of antitumor properties of effector cells stimulated with a cell wall preparation (WPG) of Bifidobacterium infantis. Biol. Pharm. Bull., 18, 148–153 (1995).
Sekine, K., Watanabe-Sekine, E., Toida, T., Kasashima, T., Kataoka, T., and Hashimoto, Y., Adjuvant activity of the cell wall of Bifidobacterium infantis for in vivo immune responses in mice. Immunopharmacol. Immunotoxicol., 16, 589–609 (1994).
Shahani, K. M. and Ayebo, A. D., Role of dietary lactobacilli in gastrointestinal microecology. Am. J. Clin. Nutr., 33, 2448–2457 (1980).
van Faassen, A., Bol, J., van den Brandt, van den Bogaard, Hermus, R. J. J., and Janknegt, R. A., Bile acids and pH values in total feces and fecal water from habitually omnivorous and vegetarian subjects. Am. J. Clin. Nutr., 58, 917–922 (1993).
Lee, W. K. and Lee, S. M., Inhibition effects of Lactic acid bacteria (LAB) on the Azoxymethance-induced colonic preneoplastic lesions. J. Microbiol., 38, 169–175 (2000).
Williams, R. T., Toxicological implications of biotransformation by intestinal microflora. Toxicol. Appl. Pharmacol., 23, 769–781 (1972).
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Kim, Y., Lee, D., Kim, D. et al. Inhibition of proliferation in colon cancer cell lines and harmful enzyme activity of colon bacteria by Bifidobacterium adolescentis SPM0212. Arch. Pharm. Res. 31, 468–473 (2008). https://doi.org/10.1007/s12272-001-1180-y
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DOI: https://doi.org/10.1007/s12272-001-1180-y