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DNA Damage and Decreased DNA Repair in Peripheral Blood Mononuclear Cells in Individuals Exposed to Arsenic and Lead in a Mining Site

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Abstract

The aim of this study was to evaluate DNA damage and the capacity for DNA repair in children exposed to arsenic and lead. During 2006, we studied a total of 85 healthy children (aged 4–11 years) who were residents of Villa de la Paz (community A), Matehuala (community B), and Soledad de Graciano Sanchez (community C) in San Luis Potosi, Mexico. The quantification of arsenic in urine (AsU) and lead in blood (PbB) was performed by atomic absorption spectrophotometry. The alkaline comet assay was used to evaluate DNA damage and DNA repair. The highest levels of AsU and PbB in children were found in community A (44.5 μg/g creatinine for arsenic and 11.4 μg/dL for lead), followed by community B (16.8 μg/g creatinine for arsenic and 7.3 μg/dL for lead) and finally by children living in community C (12.8 μg/g creatinine for arsenic and 5.3 μg/dL for lead). When DNA damage was assessed, children living in community A had the highest DNA damage. Analysis of these same cells 1 h after a challenge with H2O2 10 μM showed a dramatic increase in DNA damage in the cells of children living in community B and community C, but not in the cells of children living in community A. Moreover, significantly higher levels of DNA damage were observed 3 h after the challenge ended (repair period) in cells from individuals living in community A. Our results show that children exposed to metals might be more susceptible to DNA alterations.

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Acknowledgments

This work was supported by a grant from the Consejo Nacional de Ciencia y Tecnología, Mexico, CONACYT-SEP 24024.

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Authors and Affiliations

  1. Departamento Toxicología Ambiental, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, San Luis Potosi, Mexico

    Yolanda Jasso-Pineda, Fernando Díaz-Barriga, Jaqueline Calderón, Leticia Yáñez, Leticia Carrizales & Iván N. Pérez-Maldonado

  2. Unidad Académica Multidisciplinaria Zona Media, Universidad Autónoma de San Luis Potosí, San Luis Potosi, Mexico

    Iván N. Pérez-Maldonado

  3. Avenida Venustiano Carranza No. 2405, Col Lomas los Filtros, San Luis Potosi, 78210, SLP, Mexico

    Iván N. Pérez-Maldonado

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  1. Yolanda Jasso-Pineda
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  2. Fernando Díaz-Barriga
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  4. Leticia Yáñez
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Correspondence to Iván N. Pérez-Maldonado.

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Jasso-Pineda, Y., Díaz-Barriga, F., Calderón, J. et al. DNA Damage and Decreased DNA Repair in Peripheral Blood Mononuclear Cells in Individuals Exposed to Arsenic and Lead in a Mining Site. Biol Trace Elem Res 146, 141–149 (2012). https://doi.org/10.1007/s12011-011-9237-0

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