Abstract
PURPOSE: Colonic mucosal metabolism of butyrate may be impaired in ulcerative colitis. In this study we sought to confirm this observation, to determine if a similar change occurs in Crohn's colitis, and to establish whether a panenteric disorder of butyrate metabolism exists in either condition. METHODS: With use of a microculture technique, mucosal metabolic fluxes of14[C]-labeled butyrate and14[C]-labeled glutamine were measured as14[C] carbon dioxide production in mucosal biopsy specimens from the colon and ileum in patients with ulcerative colitis, Crohn's colitis, and healthy bowel. Results were expressed as pmol/µg biopsy DNA/hour. RESULTS: In the colon the mucosal metabolic fluxes of both butyrate and glutamine are reduced in both ulcerative colitis and Crohn's colitis compared with healthy controls. These changes were most marked in the presence of moderate to severe mucosal inflammation, there being no significant difference in mucosal metabolic flux between mildly inflamed mucosa and healthy controls. In the ileum the mucosal metabolic fluxes of butyrate and glutamine did not differ between healthy controls and those with either ulcerative colitis or Crohn's colitis. CONCLUSIONS: Changes in colonic mucosal metabolism of butyrate and glutamine in inflammatory bowel disease occur as a consequence of the inflammatory process and are not peculiar to ulcerative colitis. Ileal mucosal metabolism is unchanged in ulcerative colitis and Crohn's colitis, indicating the absence of a panenteric abnormality of mucosal metabolism in these two conditions.
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References
Roediger WE, Truelove SC. Method of preparing isolated colonic epithelial cells (colonocytes) for metabolic studies. Gut 1979;20:484–8.
Roediger WE. The colonic epithelium in ulcerative colitis: an energy deficiency disease. Lancet 1980;2:712–5.
Breuer RI, Soergel KH, Lashner BA,et al. Short chain fatty acid rectal irrigation for left-sided ulcerative colitis: a randomised, placebo controlled trial. Gut 1997;40:485–91.
Scheppach W, German-Austrian SCFA study group. Treatment of distal ulcerative colitis with short-chain fatty acid enemas. Dig Dis Sci 1997;41:2254–9.
Steinhart AH, Hiruki T, Brzezinski A, Baker JP. Treatment of left-sided ulcerative colitis with butyrate enemas: a controlled trial. Aliment Pharmacol Ther 1996;10:729–39.
Windmeiller HG, Spaeth AE. Identification of ketone bodies and glutamine as the major respiratory fuelsin vivo for post-operative rat small intestine. J Biol Chem 1978;253:69–76.
Lacey JM, Wilmore DW. Is glutamine a conditionally essential amino acid? Nutr Rev 1990;48:297–309.
Chapman MA, Grahn MF, Hutton M, Rogers J, Williams NS. Failure of colonic mucosa to oxidise butyrate in ulcerative colitis [abstract]. Gut 1992;33:S40.
Giamundo P, Grahn MF, O'Connell PR, Williams NS. Mucosal metabolism activity in endoscopic intestinal biopsy specimens. Dig Dis Sci 1991;36:541–3.
Windmeuller HG, Spaeth, AE. Intestinal metabolism of glutamine and glutamate from the lumen as compared to glutamine from blood. Arch Biochem Biophys 1975;171:662–72.
Florent C, Flourie B, Leblond A, Rautureau M, Bernier JJ, Rambaud JC. Influence of chronic lactulose ingestion on the metabolism of lactulose in man (anin-vivo study). J. Clin Invest 1985;75:608–13.
Downs TT, Wilfinger WW. Fluorimetric quantification of DNA in cells and tissue. Anal Biochem 1983;131:538–47.
Labarca C, Paigen K. A simple, rapid and selective DNA assay procedure. Anal Biochem 1980;102:344–52.
Finnie IA, Taylor BA, Rhodes JM. Ileal and colonic epithelial metabolism in quiescent ulcerative colitis: increased glutamine metabolism in distal colon but no defect in butyrate metabolism. Gut 1993;34:1552–8.
Chapman MA, Grahn MF, Boyle MA, Hutton M, Rogers J, Williams NS. Butyrate oxidation is impaired in the colonic mucosa of sufferers of quiescent ulcerative colitis. Gut 1994;35:73–6.
Roediger WE, Moore A. Effect of short chain fatty acid on sodium absorption in isolated human colon perfused through the vascular tract. Dig Dis Sci 1981;26:100–6.
Duffy MM, Regan MC, Harrington MG, O'Connell PR. Reproducible assay for colonic mucosal metabolic flux [abstract]. Ir J Med Sci 1995;164:491.
Chapman MA, Grahn MF, Hutton M, Williams NS. Butyrate metabolism in the terminal ileal mucosa of patients with ulcerative colitis. Br J Surg 1995;82:36–8.
Lewis DM, Hutton M, Grahn MF, Rogers J, Williams NS. Fuel utilisation of healthy gastric mucosa [abstract]. Br J Surg 1994;82:770.
Allan ES, Winter S, Light AM, Allan A. Mucosal enzyme activity for butyrate oxidation; no defect in patients with ulcerative colitis. Gut 1996;38:886–93.
Clausen MR, Mortensen PB. Kinetic studies on colonocyte metabolism of short-chain fatty acids and glucose in ulcerative colitis. Gut 1995;37:684–9.
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Supported by the Mater College, Dublin, Ireland.
Portions of this work were read at the American Gastroenterological Association San Francisco, California, May 19 to 24, 1996, and an abstract was published in Gastroenterology 1996;110:A900.
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Duffy, M.M., Regan, M.C., Ravichandran, P. et al. Mucosal metabolism in ulcerative colitis and crohn's disease. Dis Colon Rectum 41, 1399–1405 (1998). https://doi.org/10.1007/BF02237056
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DOI: https://doi.org/10.1007/BF02237056