Nothing Special   »   [go: up one dir, main page]

Skip to main content
Log in

Responses to environmental and chemical signals for anthocyanin biosynthesis in non-chlorophyllous corn (Zea mays L.) leaf

  • Published:
Journal of Plant Biology Aims and scope Submit manuscript

Abstract

The effects of environmental and chemical signals on anthocyanin biosynthesis in non-chlorophyllous (white) corn leaves were investigated. Carbohydrates that caused the greatest stimulation of anthocyanin formation included fructose, glucose, and sucrose, followed by maltose, raffinose, trehalose, cellobiose, melibiose, galactose, and lactose. Sucrose enhanced the expression of anthocyanin biosynthesis genes on a transcriptional level. Carbohydrate concentration, duration of light exposure, and incubation temperature also had quantitative effects. Low temperatures stimulated anthocyanin biosynthesis whereas water stress had no effect. Abscisic acid, jasmonic acid, and ethephon also enhanced anthocyanin accumulation, although the degree of its accumulation depended on co-supplied sucrose concentrations, and was relatively lower in white tissue than in green tissue. Gibberellic acids and 6-benzylaminopurine were significantly inhibitory at the nanomolar level. Indole 3-acetic acid and salicylic acid did not influence anthocyanin synthesis in the white tissue system. Diuron inhibited its formation only in green tissue. These results indicate that the white leaf segments of corn would be good systems for research on the signal networks related to chloroplast functioning in anthocyanin biosynthesis. Additionally, this experimental system could be practical for identifying hormone-like substances, especially gibberellic acids and benzylaminopurine.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Literature cited

  • Aharoni A, de Vos CHR, Wein M, Sun Z, Greco R, Kroon A, Mol JNM, O’Connell AP (2001) The strawberryFaMYB1 transcription factor suppresses anthocyanin and flavonol accumulation in transgenic tobacco. Plant J28: 319–332

    Article  PubMed  CAS  Google Scholar 

  • Chalker-Scott L (1999) Environmental significance of anthocyanins in plant stress responses. Photochem Photobiol70: 1–9

    Article  CAS  Google Scholar 

  • Christie PJ, Alfenito MR, Walbot V (1994) Impact of low-temperature stress on general phenylpropanoid and anthocyanin pathways: Enhancement of transcript abundance and anthocyanin pigmentation in maize seedlings. Planta194: 541–549

    Article  CAS  Google Scholar 

  • Deikman J, Hammer PE (1995) Induction of anthocyanin accumulation by cytokinins inArabidopsis thaliana. Plant Physiol108: 47–57

    PubMed  CAS  Google Scholar 

  • Ei-Kereamy A, Chervin C, Roustan JP, Cheynier V, Souquet JM, Moutounet M, Raynal J, Ford C, Latché A, Pech JC, Bouzayen M (2003) Exogenous ethylene stimulates the long-term expression of genes related to anthocyanin biosynthesis in grape berries. Physiol Plant119: 175–182

    Article  Google Scholar 

  • Endt DV, Kijne JW, Memelink J (2002) Transcription factors controlling plant secondary metabolism: What regulates the regulators? Phytochem61: 107–114

    Article  Google Scholar 

  • Franceschi VR, Grimes HD (1991) Induction of soybean vegetative storage proteins and anthocyanins by low-level atmospheric methyl jasmonate. Proc Natl Acad Sci USA88: 6745–6749

    Article  PubMed  CAS  Google Scholar 

  • Grotewold E, Chamberlin M, Snook M, Siame B, Butler L, Swenson J, Maddock S, St. Clair G, Bowen B (1998) Engineering secondary metabolism in maize cells by ectopic expression of transcription factors. Plant Cell10: 721–740

    Article  PubMed  CAS  Google Scholar 

  • Gyula P, Schäfer E, Nagy F (2003) Light perception and signalling in higher plants. Curr Opin Plant Biol6: 446–452

    Article  PubMed  CAS  Google Scholar 

  • Han DH, Lee SM, Lee CH, Kim SB (1996) Effects of ABA and ethephon treatments on coloration and fruit quality in ‘Kyoho’ grape. J Kor Soc Hort Sci37: 416–420

    CAS  Google Scholar 

  • Hara M, Oki K, Hoshino K, Kuboi T (2003) Enhancement of anthocyanin biosynthesis by sugar in radish (Raphanus sativus) hypocotyl. Plant Sci164: 259–265

    Article  CAS  Google Scholar 

  • Hosokawa K, Fukunaga Y, Fukushi E, Kawabata J (1996) Production of acylated anthocyanins by blue flowers ofHyacinthus orientalis regeneratedin vitro. Phytochem41: 1531–1533

    Article  CAS  Google Scholar 

  • Ilan A, Zanewich KP, Rood SB, Dougall DK (1994) Gibberellic acid decreases anthocyanin accumulation in wild carrot cell suspension cultures but does not alter 3′-nucleotidase activity. Physiol Plant92: 47–52

    Article  CAS  Google Scholar 

  • Kim JS, Lee BH, Lee JA, Oh KH, Cho KY (2003) A novel bioassay system for screening of compounds affecting anthocyanin biosynthesis pathway in white corn leaf segment. Kor J Plant Biotech30: 207–214

    Article  Google Scholar 

  • Kim JS, Yun BW, Choi JS, Kim TJ, Kwak SS, Cho KY (2004) Death mechanisms caused by carotenoid biosynthesis inhibitors in green and in undeveloped plant tissues. Pestic Biochem Physiol78: 127–139

    Article  CAS  Google Scholar 

  • Kong JM, Chia LS, Goh NK, Chia TF, Brouillard R (2003) Analysis and biological activities of anthocyanins. Phytochem64: 923–933

    Article  CAS  Google Scholar 

  • Lee SM, Han DH, Lee CH, Kim SB (1996) Effects of ABA and kinetin treatments on the coloration and quality of ‘Campbell Early’ and ‘Black Olympia’ grapes. J Kor Soc Hort Sci37: 263–268

    CAS  Google Scholar 

  • LeÓn P, Sheen J (2003) Sugar and hormone connections. Trends Plant Sci8: 110–116

    Article  PubMed  CAS  Google Scholar 

  • Mancinelli AL (1990) Interaction between light quality and light quantity in the photoregulation of anthocyanin production. Plant Physiol92: 1191–1195

    Article  PubMed  CAS  Google Scholar 

  • Martinez AE, Favret EA (1990) Anthocyanin synthesis and lengthening in the first leaf of barley isogenic lines. Plant Sci17: 35–43

    Article  Google Scholar 

  • Martinez GA, Chaves AR, Anon MC (1996) Effect of exogenous application of gibberellic acid on color change and Phenylalanine ammonia-lyse, chlorophyllase, and peroxidase activities during ripening of strawberry fruit (Fragaria xananassa Duch.). J Plant Growth Regul15: 139–146

    Article  CAS  Google Scholar 

  • Mita S, Murano N, Akaike M, Nakamura K (1997) Mutants ofArabidopsis thaliana with pleiotropic effects on the expression of the gene for β-amylase and on the accumulation of anthocyanin that are inducible by sugars. Plant J11: 841–851

    Article  PubMed  CAS  Google Scholar 

  • Moalem-Beno D, Tamari G, Leitner-Dagan Y, Borochov A, Weiss D (1997) Sugar dependent gibberellin-induced chalcone synthase gene expression in petunia corollas. Plant Physiol113: 419–424

    PubMed  CAS  Google Scholar 

  • Mol J, Jenkins G, Schäfer E, Weiss D (1996) Signal perception, transduction, and gene expression involved in anthocyanin biosynthesis. Crit Rev Plant Sci15: 525–557

    CAS  Google Scholar 

  • Müller J, Boller T, Wiemken A (1995) Trehalose and trehalase in plants: Recent developments. Plant Sci112: 1–9

    Article  Google Scholar 

  • Park EY, Kim JS, Cho KY, Pyon JY (1998) Physiology of anthocyanin accumulation in corn leaves treated with metsulfuron-methyl. Kor J Weed Sci18: 246–256

    Google Scholar 

  • Pecket RC, Small CJ (1980) Occurrence, location and development of anthocyanoplasts. Phytochem19: 2571–2576

    Article  Google Scholar 

  • Piazza P, Procissi A, Jenkins GI, Tonelli C (2002) Members ofc1/pl1 regulatory gene family mediate the response of maize aleurone and mesocotyl to different light qualities and cytokinins. Plant Physiol128: 1077–1086

    Article  PubMed  CAS  Google Scholar 

  • Rolland F, Moore B, Sheen J (2002) Sugar sensing and signaling in plants. Plant Cell14: S185-S205

    PubMed  CAS  Google Scholar 

  • Ronchi A, Farina G, Gozzo F, Tonelli C (1997) Effects of a triazolic fungicide on maize plant metabolism: Modifications of transcript abundance in resistance-related pathways. Plant Sci130: 51–62

    Article  CAS  Google Scholar 

  • Tamari G, Borochov A, Atzorn R, Weiss A (1995) Methyl jasmonate induces pigmentation and flavonoid gene expression in petunia corollas: A possible role in wound response. Physiol Plant94: 45–50

    Article  CAS  Google Scholar 

  • Wade HK, Sohal AK, Jenkins GI (2003)Arabidopsis ICX1 is a negative regulator of several pathways regulating flavonoid biosynthesis genes. Plant Physiol131: 707–715

    Article  PubMed  CAS  Google Scholar 

  • Weiss D (2000) Regulation of flower pigmentation and growth: Multiple signaling pathways control anthocyanin synthesis in expanding petals. Physiol Plant110: 152–157

    Article  CAS  Google Scholar 

  • Winkel-Shirley B (2001) Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and bio-technology. Plant Physiol126: 485–493

    Article  PubMed  CAS  Google Scholar 

  • Wright AD, Moehlenkamp CA, Perrot GH, Neuffer MG, Cone KC (1992) The maize auxotrophic mutantorange pericarp is defective in duplicate genes for tryptophan synthase. Plant Cell4: 711–719

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Jin-Seog Kim.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kim, JS., Lee, BH., Kim, SH. et al. Responses to environmental and chemical signals for anthocyanin biosynthesis in non-chlorophyllous corn (Zea mays L.) leaf. J. Plant Biol. 49, 16–25 (2006). https://doi.org/10.1007/BF03030784

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03030784

Keywords

Navigation