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Increased senile plaques without microglia in Alzheimer's disease

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Summary

To clarify the association of microglia with senile plaques, the brains from 13 patients with Alzheimer's disease (AD) and 23 nondemented aged controls were investigated immunohistochemically by a double-labeling method using anti-β-protein antiserum and anti-ferritin antibody, which is a recently reported microglia marker. In addition, a quantitative analysis was performed. The senile plaques which appeared initially in the nondemented aged controls consisted of a diffuse type without any amyloid cores and these were found in the group aged 50–59 years. The great majority of them were found to contain no ferritin-positive microglia. The number and proportion (percentage) of microglia-containing diffuse plaques increased with age. Classical and compact plaques began to appear in the brains of the group aged 70 years and over, and practically all of them contained microglia. These results suggest that microglia are not associated with initial plaque formation, but correlate with amyloid core formation. In AD, the most prominent feature was that the diffuse plaques, which contained either no or only a few ferritin-positive microglia, increased markedly.

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References

  1. Del Rio-Hortega P (1932) Microglia. In: Penfield W (ed) Cytology and cellular pathology of the nervous system, vol. 2. Hoeber, New York, pp 482–534

    Google Scholar 

  2. Dickson DW, Farlo J, Davies P, Crystal H, Fuld P, Yen S-H (1988) Alzheimer's disease: a double-labeling immunohistochemical study of senile plaques. Am J Pathol 132: 86–101

    Google Scholar 

  3. Glenner GG, Wong CW (1984) Alzheimer's disease: initial report of the purification and characterization of a novel cerebrovascular amyloid protein. Biochem Biophys Res Commun 120: 885–890

    Google Scholar 

  4. Glenner GG, Wong CW (1986) The significance of cerebrovascular amyloid in Alzheimer's disease. Neuropathol (Jpn) [Suppl] 3: 67–78

    Google Scholar 

  5. Haga S, Akai K, Ishii T (1989) Demonstration of microglial cells in and around senile (neuritic) plaques in the Alzheimer brain: an immunohistochemical study using a novel monoclonal antibody. Acta Neuropathol 77: 569–575

    Google Scholar 

  6. Hikita K, Tateishi J, Nagara H (1985) Morphogenesis of amyloid plaques in mice with Creutzfeldt-Jakob disease. Acta Neuropathol (Berl) 68: 138–144

    Google Scholar 

  7. Hsu SM, Soban E (1982) Color modification of diamino benzidine (DAB) precipitation by metallic ions and its application for double immunohistochemistry. J Histochem Cytochem 30: 1079–1082

    Google Scholar 

  8. Ikeda S, Yanagisawa N, Allsop D, Glenner G (1989) Evidence of amyloid β protein immunoreactive early plaque lesions in Down's syndrome brains. Lab Invest 61: 133–137

    Google Scholar 

  9. Itagaki S, McGeer PL, Akiyama H, Zhu S, Selkoe D (1989) Relationship of microglia and astrocytes to amyloid deposits of Alzheimer disease. J Neuroimmunol 24: 173–182

    Google Scholar 

  10. Kaneko Y, Kitamoto T, Tateishi J, Yamaguchi K (1989) Ferritin immunohistochemistry as a marker for microglia. Acta Neuropathol 79: 129–136

    Google Scholar 

  11. Kang J, Lemaire HG, Unterbeck A, Salbaum JM, Masters CL, Grzeschik KH, Multhaup G, Beyreuther K, Müller-Hill B (1987) The precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptor. Nature 325: 733–736

    Google Scholar 

  12. Khachaturian ZS (1985) Diagnosis of Alzheimer's disease. Arch Neurol 42: 1097–1104

    Google Scholar 

  13. Kidd M (1964) Alzheimer's disease: an electron microscopical study. Brain 87: 307–320

    Google Scholar 

  14. Kitamoto T, Ogomori K, Tateishi J, Prusiner SB (1987) Formic acid pretreatment enhances immunostaining of cerebral and systemic amyloids. Lab Invest 57: 230–236

    Google Scholar 

  15. Koeppen AH, Dentinger MP (1988) Brain hemosiderin and superficial siderosis of the central nervous system. J Neuropathol Exp Neurol 47: 249–270

    Google Scholar 

  16. Mannoji H, Yeger H, Becker LE (1986) A specific histochemical marker (lectin Ricinus communis agglutinin-1) for normal human microglia, and application to routine histopathology. Acta Neuropathol (Berl) 71: 341–343

    Google Scholar 

  17. Masters CL, Simms G, Weinman NA, Multhaup G, McDonald BL, Beyreuther K (1985) Amyloid plaque core protein in Alzheimer's disease and Down's syndrome. Proc Natl Acad Sci USA 82: 4245–4249

    Google Scholar 

  18. McGeer PL, Itagaki S, Tago H, McGeer EG (1987) Reactive microglia in patients with senile dementia of the Alzheimer type are positive for the histocompatibility glycoprotein HLA-DR. Neurosci Lett 79: 195–200

    Google Scholar 

  19. McGeer PL, Itagaki S, Boyes BE, McGeer EG (1988) Reactive microglia are positive for HLA-DR in the substantia nigra of Parkinson's and Alzheimer's disease brains. Neurology 38: 1285–1291

    Google Scholar 

  20. McGeer PL, Itagaki S, McGeer EG (1988) Expression of the histocompatibility glycoprotein HLA-DR in neurological disease. Acta Neuropathol 76: 550–557

    Google Scholar 

  21. Michaels J, Price RW, Rosenblum MK (1988) Microglia in the giant cell encephalitis of acquired immune deficiency syndrome proliferation, infection and fusion. Acta Neuropathol 76: 373–379

    Google Scholar 

  22. Moretz RC, Wisniewski HM, Lossinsky AS (1983) Pathogenesis of neuritic and amyloid plaques in scrapie: ultrastructural study of early changes in the cortical neuropil. In: Samuel D, Algeri S, Gershon S, Grimm VE, Toffano G (eds) Aging of the brain. Raven Press, New York, pp 61–79

    Google Scholar 

  23. Ogomori K, Kitamoto T, Tateishi J, Sato Y, Tashima T (1988) Aging and cerebral amyloid: early detection of amyloid in the human brain using biochemical extraction and immunostain. J Gerontol 43: B157-B162

    Google Scholar 

  24. Ogomori K, Kitamoto T, Tateishi J, Sato Y, Suetsugu M, Abe M (1989) β Protein amyloid is widely distributed in the central nerous system of patients with Alzheimer's disease. Am J Pathol 134: 243–251

    Google Scholar 

  25. Polak M, D'Amelio F, Johnson JE Jr, Haymaker W (1982) Microglial cells: origins and reactions. In: Haymaker W, Adams RD (eds) Histology and histopathology of the nervous system. Charles C. Thomas, Springfield, pp 481–559

    Google Scholar 

  26. Probst A, Brunnschweiler H, Lautenschlager C, Ulrich J (1987) A special type of senile plaque, possibly an initial stage. Acta Neuropathol (Berl) 74: 13–141

    Google Scholar 

  27. Prusiner SB, McKinley MP, Bowman KA, Bolton DC, Bendheim PE, Groth DF, Glenner GG (1983) Scrapie prions aggregate to form amyloid-like birefringent rods. Cell 35: 349–358

    Google Scholar 

  28. Rogers J, Luber-Nardo J, Styren SD, Civin WH (1988) Expression of immune system-associated antigens by cells of the human central nervous system: Relationship to the pathology of Alzheimer's disease. Neurobiol Aging 9: 339–349

    Google Scholar 

  29. Rozemuller JM, Eikelenboom P, Stam FC, Beyreuther K, Masters CL (1989) A4 protein in Alzheimer's discase: primary and secondary cellular events in extraccllular amyloid deposition. J Neuropathol Exp Neurol 48: 674–691

    Google Scholar 

  30. Szumanska G, Vorbrodt AW, Mandybur TI, Wisniewski HM (1987) Lectin histochemistry of plaques and tangles in Alzheimer's disease. Acta Neuropathol (Berl) 73: 1–11

    Google Scholar 

  31. Terry RD, Gonatas NK, Weiss M (1964) Ultrastructural studies in Alzheimer's presenile dementia. Am J Pathol 44: 269–297

    Google Scholar 

  32. Tomlinson BE, Blessed G, Roth M (1968) Observations on the brains of non-demented old people. J Neurol Sci 7: 331–356

    Google Scholar 

  33. Ulrich J (1985) Alzheimer changes in nondemented patients younger than sixty-five: possible early stages of Alzheimer's disease and senile dementia of Alzheimer type. Ann Neurol 17: 273–277

    Google Scholar 

  34. Wisniewski HM, Terry RD (1973) Reexamination of the pathogenesis of the senile plaque. Prog Neuropathol 2: 1–26

    Google Scholar 

  35. Wisniewski HM, Moretz RC, Lossinsky AS (1981) Evidence for induction of localized amyloid deposits and neuritic plaques by an infectious agent. Ann Neurol 10: 517–522

    Google Scholar 

  36. Wisniewski HM, Wegiel J, Wang KC, Kujawa M, Lach B (1989) Ultrastructural studies of the cells forming amyloid fibers in classical plaques. Can J Neurol Sci 16: 535–542

    Google Scholar 

  37. Yamaguchi H, Hirai S, Morimatsu M, Shoji M, Ihara Y (1988) A variety of cerebral amyloid deposits in the brains of the Alzheimer-type dementia demonstrated by β protein immunostaining. Acta Neuropathol (Berl) 76: 541–549

    Google Scholar 

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Authors and Affiliations

  1. Department of Neuropathology, Neurological Institute, Faculty of Medicine, Kyushu University, 812, Fukuoka, Japan

    T. Ohgami, T. Kitamoto, R. W. Shin, Y. Kaneko, K. Ogomori & J. Tateishi

  2. Kyowa Toxicological Research Laboratories Co., 2540 Fujimagari, 755, Ube, Yamaguchi, Japan

    T. Ohgami

Authors
  1. T. Ohgami
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  2. T. Kitamoto
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  3. R. W. Shin
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  4. Y. Kaneko
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  5. K. Ogomori
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  6. J. Tateishi
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Supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan

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Ohgami, T., Kitamoto, T., Shin, R.W. et al. Increased senile plaques without microglia in Alzheimer's disease. Acta Neuropathol 81, 242–247 (1991). https://doi.org/10.1007/BF00305864

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  • DOI: https://doi.org/10.1007/BF00305864

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