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Calmodulin-dependent and independent NAD kinase activities from cytoplasmic and chloroplastic fractions of spinach (Spinacia oleracea L.)

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Abstract

NAD kinase activity has been found in a soluble, cytoplasmic fraction and in the chloroplasts prepared from green spinach leaves. A small amount of both the cytoplasmic and the chloroplastic NAD kinase activities was retained on a calmodulin-Sepharose affinity column. The cytoplasmic NAD kinase eluted from the affinity column was found to be enhanced by calmodulin in a Ca2+-dependent manner. The chloroplastic enzyme which is located exclusively in the stroma and not in the envelope and thylakoid fractions was not affected by Ca2+ and calmodulin. The stromal fraction of purified chloroplasts contained only a negligible amount of calmodulin, most probably due to cytoplasmic contamination. Based on these data, two different mechanisms for the light-dependent modulation of spinach NAD kinase activity are suggested.

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Authors and Affiliations

  1. Laboratoire de Physiologie Végétale, Université de Genève, 3 Place de l'Université, Geneva, Switzerland

    Patrice Simon, Marc Bonzon & Hubert Greppin

  2. Institut für Biologie III, Universität Freiburg, Schänzlestr. 1, D-7800, Freiburg, Federal Republic of Germany

    Peter Dieter & Dieter Marmé

Authors
  1. Patrice Simon
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  2. Peter Dieter
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  3. Marc Bonzon
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  4. Hubert Greppin
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  5. Dieter Marmé
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Simon, P., Dieter, P., Bonzon, M. et al. Calmodulin-dependent and independent NAD kinase activities from cytoplasmic and chloroplastic fractions of spinach (Spinacia oleracea L.). Plant Cell Reports 1, 119–122 (1982). https://doi.org/10.1007/BF00272368

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  • DOI: https://doi.org/10.1007/BF00272368

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