Abstract
One great experimental advantage of the patch-clamp technique is that it permits the recording of single-channel currents from isolated cell-free patches of membrane. By appropriate manipulations, it is possible to obtain cell-free patches in which either the normal external surface of the membrane is towards the bath solution, termed an outside-out patch, or the cytoplasmic surface of the membrane is towards the bath solution, termed an inside-out patch (Hamill et al., 1981). So far, inside-out membrane patches have been employed to study the effects of various intracellular ions on both current flow through open channels (Horn and Patlak, 1980) and channel gating (Marty, 1981; Colquhoun et al., 1981; Yellen, 1982). Although outside-out membrane patches have been used to a lesser extent, they provide a convenient means of studying the effects on channels of various drugs (including neurotransmitters) applied to the outside of the membrane.
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References
Anderson, C. R., and Stevens, C. F., 1973, Voltage clamp analysis of acetylcholine produced end-plate current fluctuations at frog neuromuscular junction,J. Physiol. (Lond.) 235:665–691.
Colquhoun, D., and Hawkes, A. G., 1977, Relaxation and fluctuations of membrane currents that flow through drug-operated channels, Proc. R. Soc. Lond. [Biol.] 199:231–262.
Colquhoun, D., and Sakmann, B., 1981, Fluctuations in the microsecond time range of the current through single acetylcholine receptor ion channels, Nature 294:464–466.
Colquhoun, D., Neher, E., Reuter, H., and Stevens, C. F., 1981, Inward current channels activated by intracellular Ca in cultured cardiac cells, Nature 294:752–754.
Hamill, O. P., Marty, A., Neher, E., Sakmann, B., and Sigworth, F. J., 1981, Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches, Pfluegers Arch. 391:85–100.
Horn, R., and Patlak, J., 1980, Single channel currents from excised patches of muscle membrane, Proc. Natl. Acad. Sci. U.S.A. 77:6930–6934.
Katz, B., and Miledi, R., 1972, The statistical nature of the acetylcholine potential and its molecular components, J. Physiol. (Lond.) 224:665–699.
Koenig, J., Oren, M., and Melone, M. A. B., 1982, Establishment of neuromuscular contacts in cultures of rat embryonic cells: Effect of tetrodotoxin on maturation of muscle fibers and on formation and maintenance of acetylcholinesterase and acetylcholine receptor clusters, Dev. Neurosci. 5:314–325.
Marty, A., 1981, Ca-dependent K channels with large unitary conductance in chromaffin cell membranes, Nature 291:497–500.
Seeman, P., 1972, The membrane actions of anesthetics and tranquilizers, Pharmacol. Rev. 24:583–655.
Yellen, G., 1982, Single Ca++-activated nonselective cation channels in neuroblastoma, Nature 296:357–359.
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© 1983 Plenum Press, New york
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Trautmann, A., Siegelbaum, S.A. (1983). The Influence of Membrane Patch Isolation on Single Acetylcholine-Channel Current in Rat Myotubes. In: Sakmann, B., Neher, E. (eds) Single-Channel Recording. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7858-1_25
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DOI: https://doi.org/10.1007/978-1-4615-7858-1_25
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4615-7860-4
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