Abstract
CD24 antigen is a glycoprotein expressed on haematopoietic cells, including B cells, T cells and granulocytes and on non-haematopoietic cells, including neural cells, ganglion cells and the cells of the adrenal medulla. The antigen is also expressed on renal cell carcinoma, small cell lung carcinoma and neuroblastoma. We have cloned rat cDNA encoding core polypeptide of CD24 antigen from embryonic brain and shown that the core molecule is highly expressed in embryonic brain and non-neural tissues. Rat tissue and various human neoplastic cell lines were investigated for the gene expression of CD24 core polypeptide by in situ hybridization. The transcript was localized in gastrointestinal epithelia, ductal and acinar epithelia of the salivary gland, the bronchiolar epithelium, renal tubular epithelium, the epithelium of the oviduct, follicular cells of the thyroid, medullary cells of the adrenal gland, Auerbach's plexus, B blastoid cells in lymph nodes, hair follicles, and the sweat glands of the skin. Among the various human neoplastic cell lines investigated, the transcript was detected in squamous cell carcinoma of the lung, gastric carcinoma, colon carcinoma, choriocarcinoma and renal cell carcinoma. The result suggest that the core molecule of CD24 antigen may be expressed in a wider range of epithelial cells and carcinoma cell lines than has been reported. Furthermore, we show that gene expression of CD24 core polypeptide is confined to the proliferative zone of the gastrointestinal mucosa, suggesting that core molecule is transiently expressed on the surface of epithelial cells in the process of cellular maturation. We discuss a possible role for CD24 antigen in the maturation of epithelial cells in the gastrointestinal tract.
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References
Abramson CS, Kersey JH, LeBien TW (1981) A monoclonal antibody (BA-1) reactive with cells of human B lymphocyte lineage. J Immunol 126:83–88
Bertalanffy FD, Lau C (1963) Mitotic rates, renewal times, and cytodynamics of the female genital tract epithelia in the rat. Acta Anat 54:39–81
Borowitz MJ, Weiss MA, Bossen EH, Metzgar RS (1986) Characterizaition of renal neoplasms with monoclonal antibodies to leukocyte differentiation antigens. Cancer 57:251–256
Brito M J, Filipe M I, Morris RW (1992) Cell proliferation study on gastric carcinoma and non-involved gastric mucosa using a bromodeoxyuridine (BrdU) labelling technique. Eur J Cancer Prev 1:429–435
Bruce J, Symington FW, Mckearn TJ, Sprent J (1981) A monoclonal antibody discriminating between subsets of T and B cells. J Immunol 127:2496–2501
Chomzynski P, Sacci N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156–159
Crispe IN, Bevan MJ (1987) Expression and functional significance of the J11d marker on mouse thymocytes. J Immunol 138:2013–2018
Droz D, Zachar K, Charbit L, Gogusev J, Chrétien Y, Iris L (1990) Expression of the human nephron differentiation molecules in renal cell carcinomas. Am J Pathol 137:895–905
Fischer GF, Majdic O, Gadd S, Knapp W (1990) Signal transduction in lymphocytic and myeloid cells via CD24, a new member of phosphoinositol-anchored membrane molecules. J Immunol 144:638–641
Hattori T, Helpap B, Gedigt P (1983) Development and cell kinetics of colonic tumors induced in mice by dimethylhydrazine. J Cancer Res Clin Oncol 105:148–157
Jackson D, Waibel R, Weber E, Bell J, Stahel RA (1992) CD24, a signal-transducing molecule expressed on human B cells, is a major surface antigen on small cell lung carcinomas. Cancer Res, 52:5264–5270
Kay R, Takei F, Humphries RK (1990) Expression cloning of a cDNA encoding M1/69-J11d heat-stable antigens. J Immunol 145:1952–1959
Kay R, Rosten PM, Humphries RK (1991) CD24, A signal transducer modulating B cell activation responses, is a very short peptide with a glycosyl phosphatidylinositol membrane anchor. J Immunol 147:1412–1416
Kemshead JT, Fritschy J, Asser U, Sutherland R, Greaves MF (1982) Monoclonal antibodies defining markers with apparent selectivity for particular haemopoietic cell types may also detect antigens on cells of neural crest origin. Hybridoma 1:109–123
Knapp W, Majdic O, Bettelheim P, Liszka K, Aberer W, Stingl G (1983) Typing of leukemic cells with monoclonal antibodies. Ann NY Acad Sci 420:251–260
Larkin M, Knapp W, Stoll MS, Mehmet H, Feizi T (1991) Monoclonal antibodies VIB-E3, IB5 and HB9 to the leucocyte/epithelial antigen CD24 resemble BA-1 in recognizing sialic acid-dependent epitope(s). Evidence that VIB-E3 recognizes NeuAca2–6GalNAc and NeuACa2–6Gal sequences. Clin Exp Immunol 85:536–541
Lehmann S, Kuckler S, Theveniau M, Vincendon G, Zanetta JP (1990) An endogenous lectin and one of its neuronal glycoprotein ligands are involved in contact guidance of neuron migration. Proc Natl Acad Sci USA 87:6455–6459
Mechtersheimer G (1991) Towards the phenotyping of soft tissue tumours by cell surface molecules. Virchow Arch [A] 419:7–28
Miller BA, Antognetti G, Springer TA (1985) Identification of cell surface antigens present on murine hematopoietic stem cells. J Immunol 134:3286–3290
Mittler RS, Talle MA, Carpenter K, Rao PE, Goldstein G (1983) Generation and characterization of monoclonal antibodies reactive with human B lymphocytes. J Immunol 131:1754–1761
Platt JL, LeBien TW, Michael AF (1983) Stages of renal ontogenesis identified by monoclonal antibodies reactive with lymphohemopoietic differentiation antigens. J Exp Med 157:155–172
Shirasawa T, Sakamoto K, Akashi T, Takahashi H, Kawashima A (1992) Nucleotide sequence of rat elongation factor-1 alpha cDNA. Nucleic Acids Res 20:909
Shirasawa T, Akashi T, Sakamoto K, Takahashi H, Maruyama N, Hirokawa K (1993) Gene expression of CD24 core peptide molecule in developing brain and developing non-neural tissues. Dev Dyn 198:1–13
Tokunaga K, Taniguchi H, Yoda K, Shimizu M, Sakiyama S (1986) Nucleotide sequence of a full-lenth cDNA for mouse cytoskeletal β-actin mRNA. Nucleic Acids Res 14:2829
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Akashi, T., Shirasawa, T. & Hirokawa, K. Gene expression of CD24 core polypeptide molecule in normal rat tissues and human tumor cell lines. Vichows Archiv A Pathol Anat 425, 399–406 (1994). https://doi.org/10.1007/BF00189578
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DOI: https://doi.org/10.1007/BF00189578