Abstract
The epidermis is characterised by its self-ability to maintain its homeostasis through a controlled balance between keratinocyte proliferation and differentiation events. Therefore, the specific detection of proliferation- and differentiation markers allowing the discrimination between these epidermal activities is of great interest. Proliferation markers are numerous and the most frequently used, including nuclear and cytoplasmic markers, are described in this chapter. From the use of tritiated thymidine and BrdU to the detection of PCNA, histones, Ki67 and keratins, all approaches are described with their technological procedures. Markers of the different steps of epidermal differentiation markers such as keratins, filaggrin, involucrin and loricrin are then listed including stained skin images, antibodies references, methods of use and suppliers.
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Annex
Annex
There are many suppliers of antibodies, and the number of available antibodies (immunohistology, cytometry, etc.) regularly increases. Some suppliers seek worldwide for a specific product if wanted by the customer.
Ask for the technical bulletin of each product. This indicates the recommended method (histology, electrophoresis, cytometry, etc.) and conditions of use (fixation, congelation, treatment, etc.). Dilutions are just indicative and must be evaluated for each tissue and protocol.
Immunohistological studies are performed according to standard methodologies, with or without amplification or treatment. Many kits are actually available.
Some addresses of suppliers or distributors are given below. This list is far from being complete.
Biomedical Technologies, Inc., 378 Page Street. Stoughton. MA 02072 USA
Phone: 617-344-9942
Biosource International (Tagoimmunologicals°) 820 Flynn Road Camarillo CA 93012 USA
Phone: 1-805-987-0086
Dako Corporation. 6392 Via Real. Carpinteria CA 93013 USA
Phone: 1-805-566-6655
Pierce P.O Box 117. Rockford Illinois 61105 USA
Phone: 1-800-842-5007
Roche Diagnostics Corporation. 9115 Hague Road. P.O Box 50414. Indianapolis IN 46250-0414 USA
Phone: 800-428-5433
Sigma P.O Box 14508 St Louis Missouri 63178 USA
Phone: 800-325-3010
Southern Biotechnology Associates, Inc., P.O Box 26221. Birmingham. Alabama 35260 USA
Phone: 205-945-1774
Zymed Laboratories, Inc., 458 Carlton Court. South San Francisco CA 940 80–2012 USA
Phone: 800- 874–4494
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Rousselle, P., Gentilhomme, E., Neveux, Y. (2017). Markers of Epidermal Proliferation and Differentiation. In: Humbert, P., Fanian, F., Maibach, H., Agache, P. (eds) Agache's Measuring the Skin. Springer, Cham. https://doi.org/10.1007/978-3-319-32383-1_37
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DOI: https://doi.org/10.1007/978-3-319-32383-1_37
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