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Multifunctional electrospun polyvinyl alcohol/gellan gum/polycaprolactone nanofibrous membrane containing pentoxifylline to accelerate wound healing

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Abstract

In this research, a novel drug-loaded nanofibrous membrane composed of polyvinyl alcohol/gellan gum (PVA/GG) on polycaprolactone (PCL) as a scaffold to deliver pentoxifylline (PTX) was fabricated for wound healing. The morphology and mean fiber diameter of scaffolds were characterized. Mechanical properties, wettability, degradation rate, and drug delivery were evaluated for each fibrous scaffold. The cytotoxicity evaluation of the samples was conducted using human dermal fibroblasts (HDFs). The results confirmed that PVA/GG with the ratio of 50:50 has an optimum fibers’ diameter ranging between 86 and 110 nm, over 76% of porosity, and a desired mechanical properties for skin tissue engineering. Ultimate tensile strength (UTS) and elastic modulus of the PTX-loaded scaffold (PVA/GG 50:50) decreased compared with the non-loaded one. Adding 20 mg/ml PTX to the scaffold caused a considerable increase in the samples’ degradation. Furthermore, the PTX-loaded scaffold showed a higher wettability and roughness in comparison with the one without PTX. The PTX was released from the fibrous membrane up to 120 h. HDFs’ viability and adhesion were significantly higher for drug-loaded scaffolds compared with the control group. In summary, the nanofibrous composite scaffold made of PTX-PVA-GG/PCL could be used as a suitable wound dressing for speeding up wound regeneration.

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Acknowledgements

The authors gratefully acknowledge the support for this work by the University of Isfahan (financial and scientific) and Tehran University of Medical Sciences (scientific).

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Authors and Affiliations

  1. Department of Biomedical Engineering, Faculty of Engineering, University of Isfahan, Isfahan, Iran

    Zahra Shahravi & Mehdi Mehdikhani

  2. Skin and Stem Cell Research Centre, Tehran University of Medical Sciences, Tehran, Iran

    Mohammad Amir Amirkhani

  3. Stem Cell and Regenerative Medicine Center of Excellence, Tehran University of Medical Sciences, Tehran, Iran

    Mohammad Amir Amirkhani

  4. Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

    Mahsa Mollapour Sisakht

  5. Department of Biochemistry, Erasmus University Medical Center, PO BOX 20403000CA, EE634, Rotterdam, Netherlands

    Mahsa Mollapour Sisakht

  6. Department of Clinical Pharmacy and Pharmacy Practice, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran

    Shadi Farsaei

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Shahravi, Z., Mehdikhani, M., Amirkhani, M.A. et al. Multifunctional electrospun polyvinyl alcohol/gellan gum/polycaprolactone nanofibrous membrane containing pentoxifylline to accelerate wound healing. Polym. Bull. 80, 2217–2237 (2023). https://doi.org/10.1007/s00289-022-04446-1

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