Papers by Brazhnikova Yelena
Acta Biochimica Polonica, 2016
In this study, 382 isolates of bacterial endophytes from tissues of plants grown in the foothills... more In this study, 382 isolates of bacterial endophytes from tissues of plants grown in the foothills and piedmont plains of the Trans-Ili Alatau were isolated. It was found that certain strains actively produce indole-3-acetic acid (IAA) in a medium containing l-tryptophan. Among the strains studied, 26 strains (66%) showed a positive response to production of IAA. Bacteria synthesized IAA in the range of 18.6 ± 1.1 to 82.4 ± 2.3 µg/ml. IAA was synthesized more actively by Streptosporangium sp. KK1 (44.1 µg/ml), Rhodococcus sp. KK 2 (42.5 µg/ml), Streptomyces tendae KK3 (44.9 µg/ml) strains. The most active auxin's producer is a Jeotgalicoccus halotolerans BAK1 strain, whose total level of IAA production reached 82.4 µg/ml. Such strains as Streptomyces griseorubiginosis KK4, Streptomyces sp. KK5 and Jeotgalicoccus halotolerans BAK1 were found to have a significant stimulating effect which was reflected in the increase of the length of the roots of soybean and barley. As a result, 8...
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Reseach Paper, 2015
538 yeast strains were isolated from dark chestnut soil collected from under the plants of the le... more 538 yeast strains were isolated from dark chestnut soil collected from under the plants of the legumefamily (Fabaceae). The greatest number of microorganisms is found at soil depth 10–20 cm. Among the 538strains of yeast 77 (14.3%) strains demonstrated the ability to synthesize IAA. 15 strains were attributedto high IAA-producing yeasts (above 10 g/ml). The most active strains were YA05 with 51.7 ± 2.1 g/mlof IAA and YR07 with 45.3 ± 1.5 g/ml. In the study of effect of incubation time on IAA production themaximum accumulation of IAA coincided with maximum rates of biomass: at 120 h for YR07 and at144 h for strain YA05. IAA production increased when medium was supplemented with the l-tryptophan.400 g/ml of l-tryptophan showed maximum IAA production. 10 strains demonstrated the ability toinhibit the growth and development of phytopathogenic fungi. YA05 and YR07 strains formed the largestzones of inhibition compared to the other strains – from 21.6 ± 0.3 to 30.6 ± 0.5 mm. Maximum zoneof inhibition was observed for YA05 against Phytophtora infestans and YR07 strains against Fusariumgraminearum. YA05 and YR07 strains were identified as Aureobasidium pullulans YA05 (GenBank accessionNo JF160955) and Rhodotorula mucilaginosa YR07 (GenBank accession No JF160956).
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Reseach Paper, 2015
The possibility of application of micromycetes (strains
Penicillium bilaiae Pb14, Aureobasidium p... more The possibility of application of micromycetes (strains
Penicillium bilaiae Pb14, Aureobasidium pullulans YA05
and Rhodotorula mucilaginosa YR07) to increase yields of
soybean (Glycine max cv Almaty) and barley (Hordeum
vulgare cv Arna) was estimated. It was shown that the
most positive effect on germination energy and seed
germination after seed treatment with liquid culture,
supernatant and filtrate, is achieved at 1:5 dilution. In
studying the influence of cell-associated and extracellular
biologically active compounds of micromycetes (liquid
culture and supernatant) on biometric parameters of
seedlings, the maximum stimulating effect was observed
in the variants with liquid culture. These strains of micromycetes
were used as a bases for various compositions
of preparations — application of each strain separately
and application of micromycetes mixes. In microfield experiments,
the increase of soybean yield ranged from 4.5
to 9.4 quintal/ha, barley — from 2.9 to 5.9 quintal/ha.
A significant increase in various parameters of structure
of the yield was shown in all experimental variants when
compared to the control. It was found that an increase
in soybean and barley yields and yield components was
higher in the variant with a mix of micromycetes when
compared to the separate application of each strain. The
most efficient mixture was based on the mix of fungal
strains (culture filtrate of P. bilaiae Pb14 diluted 1:5 +
liquid cultures of A. pullulans YA05 and Rh. mucilaginosa
YR07 in a 1:5 dilution)
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Papers by Brazhnikova Yelena
Penicillium bilaiae Pb14, Aureobasidium pullulans YA05
and Rhodotorula mucilaginosa YR07) to increase yields of
soybean (Glycine max cv Almaty) and barley (Hordeum
vulgare cv Arna) was estimated. It was shown that the
most positive effect on germination energy and seed
germination after seed treatment with liquid culture,
supernatant and filtrate, is achieved at 1:5 dilution. In
studying the influence of cell-associated and extracellular
biologically active compounds of micromycetes (liquid
culture and supernatant) on biometric parameters of
seedlings, the maximum stimulating effect was observed
in the variants with liquid culture. These strains of micromycetes
were used as a bases for various compositions
of preparations — application of each strain separately
and application of micromycetes mixes. In microfield experiments,
the increase of soybean yield ranged from 4.5
to 9.4 quintal/ha, barley — from 2.9 to 5.9 quintal/ha.
A significant increase in various parameters of structure
of the yield was shown in all experimental variants when
compared to the control. It was found that an increase
in soybean and barley yields and yield components was
higher in the variant with a mix of micromycetes when
compared to the separate application of each strain. The
most efficient mixture was based on the mix of fungal
strains (culture filtrate of P. bilaiae Pb14 diluted 1:5 +
liquid cultures of A. pullulans YA05 and Rh. mucilaginosa
YR07 in a 1:5 dilution)
Penicillium bilaiae Pb14, Aureobasidium pullulans YA05
and Rhodotorula mucilaginosa YR07) to increase yields of
soybean (Glycine max cv Almaty) and barley (Hordeum
vulgare cv Arna) was estimated. It was shown that the
most positive effect on germination energy and seed
germination after seed treatment with liquid culture,
supernatant and filtrate, is achieved at 1:5 dilution. In
studying the influence of cell-associated and extracellular
biologically active compounds of micromycetes (liquid
culture and supernatant) on biometric parameters of
seedlings, the maximum stimulating effect was observed
in the variants with liquid culture. These strains of micromycetes
were used as a bases for various compositions
of preparations — application of each strain separately
and application of micromycetes mixes. In microfield experiments,
the increase of soybean yield ranged from 4.5
to 9.4 quintal/ha, barley — from 2.9 to 5.9 quintal/ha.
A significant increase in various parameters of structure
of the yield was shown in all experimental variants when
compared to the control. It was found that an increase
in soybean and barley yields and yield components was
higher in the variant with a mix of micromycetes when
compared to the separate application of each strain. The
most efficient mixture was based on the mix of fungal
strains (culture filtrate of P. bilaiae Pb14 diluted 1:5 +
liquid cultures of A. pullulans YA05 and Rh. mucilaginosa
YR07 in a 1:5 dilution)