Papers by Julio V Figueroa
Transboundary and Emerging Diseases
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Frontiers in Veterinary Science
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Pathogens
Bovine babesiosis is a tick-borne disease of cattle caused by the protozoan parasites of the genu... more Bovine babesiosis is a tick-borne disease of cattle caused by the protozoan parasites of the genus Babesia. Babesia bovis, Babesia bigemina and Babesia divergens are considered by International health authorities (OIE) as the principal species of Babesia that cause bovine babesiosis. Animals that recover from a babesial primo infection may remain as persistent carriers with no clinical signs of disease and can be the source of infection for ticks that are able to acquire Babesia parasites from infected cattle and to transmit Babesia parasites to susceptible cattle. Several procedures that have been developed for parasite detection and diagnosis of this infectious carrier state constitute the basis for this review: A brief description of the direct microscopic detection of Babesia-infected erytrocytes; PCR-based diagnostic assays, which are very sensitive particularly in detecting Babesia in carrier cattle; in-vitro culture methods, used to demonstrate presence of carrier infections ...
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Parasitol Res, 2000
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Annals of the New York Academy of Sciences
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Bolio González, M.E., Figueroa Millán, J.V., Álvarez Martínez, J.A., Rojas Martínez, C., Vega y Murguía, C.A., López Rojas M. (2015). Capítulo 4: Examen de laboratorio para parásitos de la sangre. En: Técnicas para el diagnóstico de parásitos con importancia en salud pública y veterinaria. Rodríg...
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Veterinaria Mexico
The effect of Lactobacillus casei on INIFAP's mixed vaccine against bovine babesiosis (VAC) w... more The effect of Lactobacillus casei on INIFAP's mixed vaccine against bovine babesiosis (VAC) was assessed in bovines in an endemic babesiosis area. It was previously reported that L. casei increases the efficiency of the Mexican mixed vaccine against bovine babesiosis under controlled conditions. The results of the present study demonstrated the effectiveness of simultaneous vaccination of bovines with L. casei and the mixed vaccine against bovine babesiosis in eliciting a protective immune response under extreme conditions in the field. Twenty Babesia spp free bovines were allocated into three groups: un-vaccinated (Control, n = 9), vaccinated with VAC (n = 5), and vaccinated simultaneously with VAC and Lactobacillus casei (LC-VAC, n = 6). All animals were kept in a tick and Babesia spp free field at Coatepec, Veracruz during 24 days before moving them to Paso del Toro, Veracruz, for a natural exposition to Babesia spp transmitted by Riphicephalus (Boophilus) microplus ticks. Pr...
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Tecnica Pecuaria en Mexico
Se determinó la capacidad imnunoprotectora de una cepa atenuada de B. bigemina en bovinos. Se uti... more Se determinó la capacidad imnunoprotectora de una cepa atenuada de B. bigemina en bovinos. Se utilizaron 24 bovinos de un año de edad provenientes de zona libre de garrapatas. La cepa imnunizante de B. bigemina fue aislada de un caso clinico y se ha atenuado por mantenimiento en cultivo in vitro. Para Ia determinación de la dosis minima inmunizante se conformaron cinco grupos de cuatro animales que recibieron dosis crecientes de 10 5-10 9 de eritrocitos inIectados, y un grupo testigo sin vacunacion. Los animales vacunados y desafiados fueron monitoreados registrando temperatura rectal, volumen celular aglomerado (VCA) y parasitemia. En la fase posvacunal no hubo cambios notorios en los parámetros indicados. La confrontación se realizó siete meses posvacunación utilizando resultados de un ensayo previo en el que se determinó como dosis de confrontación 10 8 eritrocitos inIectados con aislado de campo por via intramuscular. Al dia ocho posconfrontación se observaron parásitos circulan...
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Parassitologia, 2007
The severe negative impact that bovine babesiosis has in the Mexican cattle industry has not been... more The severe negative impact that bovine babesiosis has in the Mexican cattle industry has not been ameliorated basically due to the lack of safe and effective commercially available vaccines and sensitive and reliable diagnostic tests. In recent years, the Bovine Babesiosis Laboratory at the National Center for Disciplinary Research in Veterinary Parasitology-INIFAP in Morelos State, Mexico has been directing efforts towards three main research areas: (1) The development of in vitro culture-derived, improved and safer live vaccines. This has been done in two ways: using gamma-irradiated bovine serum and erythrocytes for the in vitro culture of vaccine strains, which reduces the risk of contaminating pathogens, and improving the immune response, by the addition of L. casei, a strong stimulant of the innate immune system. (2) The study of antigens considered as vaccine candidates with the goal of developing a recombinant vaccine that suits the country's needs. Knowing their degree ...
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Revista latinoamericana de microbiología
To measure the antibody response to Babesia bigemina with an ELISA test, three groups of cattle w... more To measure the antibody response to Babesia bigemina with an ELISA test, three groups of cattle were experimentally infected with two isolates of the parasite. It was possible to demonstrate specific antibody binding directed against the parasite as early as the 7 days postinfection (PI). The highest level of antibody was obtained around day 1 to 23 and remained detectable for 260 days. Challenge of the animals 260 days PI with a tick-induced B. bigemina infection depicted that homologous strain-challenged calves did not show an increase of IgG antibody levels, where as those challenged with the heterologous isolate did. In the latter groups the resulting level of antibodies was even higher than after the primary infection. The immunoblotting technique showed that the antibody response is probably directed against groups of B. bigemina components with a relative mobilities of 68-64 kDa, 62-54 kDa and 52-42 kDa, which appear to be major components of the protozoa. By observing the cr...
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Revista latinoamericana de microbiología
A Duplex Polymerase Chain Reaction (DPCR)/DNA probe assay was used to detect Babesia bovis and B.... more A Duplex Polymerase Chain Reaction (DPCR)/DNA probe assay was used to detect Babesia bovis and B. bigemina DNA in cattle undergoing immunization trials. Blood samples were collected from 15 non-splenectomized, 1-2 years old bulls, inoculated with 1 x 10(7) each of culture-derived B. bovis- and B. bigemina-infected erythrocytes. 15 bulls inoculated with normal erythrocytes served as a control group. All cattle were field exposed to tick-transmitted Babesia 21 days (20 animals, Group I) and 60 days (10 animals, Group II) post-inoculation (PI). After immunization, the DPCR/DNA probe assay detected B. bigemina and B. bovis parasite DNA in all inoculated animals from days 4 to 14 PI. At challenge, B. bovis DNA was detected in all control animals as early as day 8 (Group I), or day 11 (Group II) post-introduction to a tick-infested area. The immunized bulls showed B. bovis positive PCR/DNA probe signals from day 0 (Group II) and day 8 (group I), up to day 32 post-exposure to ticks. Positi...
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Experimental & applied acarology, 1999
Ticks play an important role in human and veterinary medicine, in particular due to their ability... more Ticks play an important role in human and veterinary medicine, in particular due to their ability to transmit a wide spectrum of pathogenic micro-organisms of protozoal, rickettsial, bacterial and viral origin. Pathogens in ticks can be identified by conventional methods such as indirect immunofluorescence, isolation in cell culture or by using histological staining techniques. However, the advent of the polymerase chain reaction (PCR) has resulted in tremendous improvements in the specific and sensitive detection of pathogen DNA in ticks. In this paper, literature on DNA extraction methods, PCR protocols, primers and probes, which are in use for the successful detection and identification of pathogens in ticks, are critically reviewed. Some recommendations are also given towards the end of this review.
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Revue d'élevage et de médecine vétérinaire des pays tropicaux, 1993
A study was conducted to test the applicability of a Polymerase Chain Reaction (PCR)-based approa... more A study was conducted to test the applicability of a Polymerase Chain Reaction (PCR)-based approach for the simultaneous detection of the bovine hemoparasites Babesia bigemina, B. bovis and Anaplasma marginale. Bovine blood samples from cattle ranches of a previously determined enzootic zone in the Yucatan Peninsula of Mexico, were collected from peripheral blood and processed for PCR analysis. Blood samples were subjected to DNA amplification by placing an aliquot in a reaction tube containing oligonucleotide primers specific for DNA of each hemoparasite species. The PCR products were detected by Dot-Blot nucleic acid hybridization utilizing nonradioactive, species-specific, digoxigenin PCR-labeled DNA probes. Four hundred twenty one field samples analyzed by the multiplex PCR-DNA probe assay showed 66.7%, 60.1% and 59.6% prevalence rates for B. bigemina, B. bovis and A. marginale, respectively. The multiplex PCR analysis showed that animals with single, double or triple infection ...
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Parasitology Research, 1990
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Parasitology Research, 2000
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Vet. Mex, 2003
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Annals of the New York Academy of Sciences, 2004
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Annals of the New York Academy of Sciences, 2004
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Annals of the New York Academy of Sciences, 2004
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Papers by Julio V Figueroa