Papers by virginie Marquis
Chemical Research in Toxicology, 2004
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www.mdpi.com/journal/toxins
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Mutagenesis, 2014
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Toxins, 2013
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Ecotoxicology and Environmental Safety, 2015
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Mutagenesis, 2012
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Molecular Nutrition & Food Research, 2006
Ochratoxin A (OTA), a nephrotoxic mycotoxin probably implicated in human Balkan endemic nephropat... more Ochratoxin A (OTA), a nephrotoxic mycotoxin probably implicated in human Balkan endemic nephropathy and associated urothelial tumors, induces renal carcinomas in rodents and nephrotoxicity in pigs. OTA induces DNA-adduct formation, but the structure of the adducts and their role in nephrotoxicity and carcinogenicity have only partly been elucidated. In vivo, 2-mercaptoethane sulfonate (MESNA) protects rats against OTA-induced nephrotoxicity but not against carcinogenicity, indicating two different mechanisms leading to nephrotoxicity or carcinogenicity. To better understand how DNA-adduct could be generated, opossum kidney cells (OK) have been treated by OTA alone or in presence of several compounds such as MESNA or N-acetylcysteine (another agent that, like MESNA, reduces oxidative stress by increasing of free thiols in kidney), buthionine sulfoximine (BSO) (an inhibitor of glutathione-synthase), and alpha amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid (ACIVICIN) (an inhibitor of gamma glutamyl transpeptidase). Cytotoxicity of OTA on OK cells was evaluated by applying the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. None of the listed agents diminished OTA cytotoxicity significantly; ACIVICIN even increases OTA cytotoxicity. In contrast, analysis of the HPLC profiles of OTA metabolites produced during these incubations indicated that the pattern, the quantity of metabolites, and the nature of the derivatives were modulated by these agents. Ochratoxin B (OTB), open-ring ochratoxin A (OP-OA), 4 hydroxylated OTA, 10 hydroxylated OTA, OTA without phenylalanine, OTB without phenylalanine, and a dechlorinated OTA metabolite could be identified by nano-ESI-IT-MS.
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Journal of Membrane Science, 2010
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Chemical Research in Toxicology, 2006
Ochratoxin A (OTA) is a mycotoxin that shows potent nephrotoxicity and renal carcinogenicity in r... more Ochratoxin A (OTA) is a mycotoxin that shows potent nephrotoxicity and renal carcinogenicity in rodents. One hypothesis for OTA-induced tumor formation is based on its genotoxic properties that are promoted by oxidative metabolism. Like other chlorinated phenols, OTA undergoes an oxidative dechlorination process to generate a quinone (OTQ)/hydroquinone (OTHQ) redox couple that may play a role in OTA-mediated genotoxicity. To determine whether the OTQ/OTHQ redox couple of OTA contributes to genotoxicity, the DNA adduction properties, as evidenced by the (32)P-postlabeling technique, of the hydroquinone analogue (OTHQ) have been compared to OTA in the absence and presence of metabolic activation (pig kidney microsomes) and within human bronchial epithelial (WI26) and human kidney (HK2) cells. Our experiments show that OTHQ generates DNA adduct spots in the absence of metabolic activation. These adducts are ascribed to covalent DNA adduction by OTQ generated through autoxidation of the hydroquinone precursor, OTHQ. Although OTA does not interact with DNA in the absence of metabolism, the OTQ-mediated DNA adduct spots noted with OTHQ are also observed with OTA following treatment with pig kidney microsomes and NADPH, suggesting that OTA undergoes oxidative activation to OTQ by cytochrome P450 or enzymes with peroxidase activity. Comparison of DNA adduction by OTHQ and OTA in human cell lines shows that OTQ-mediated adduct spots form in a dose- and time-dependent manner. The adduct spots form at a faster rate with OTHQ, which is consistent with more facile generation of OTQ from its hydroquinone precursor. These results establish structure-activity relationships for OTA-mediated DNA adduction and provide new evidence for the potential role of the OTQ/OTHQ redox couple in OTA-induced genotoxicity.
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Bioresource Technology, 2011
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Chemical Research in Toxicology, 2010
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The growing use of antineoplastic drugs in cancer therapy is an emerging issue in environmental r... more The growing use of antineoplastic drugs in cancer therapy is an emerging issue in environmental research. The presence of the anticancer drug cyclophosphamide (CP) in municipal wastewater raises several environmental problems. Besides its cytotoxic effects, CP possesses teratogenic and mutagenic properties and is a known human carcinogen. The application of membrane bioreactor (MBR) technology was investigated with the aim of evaluating its potential for cytostatic drug bioremoval. The toxicity removal was assessed from biomarkers tests and related to the choice of the reactor operating conditions. The influence of sludge retention times (SRT) on CP removal was suited but not significant effects were found for variation of SRT from 50 days to 70 days. CP removal up to 80% was achieved under studied operating conditions. In front of such pollution, evidence has been made about the use of MBR. Our study proofed that advances wastewater treatment using a MBR provides a suitable process...
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La presence de medicaments cytostatique a ete evaluee en sortie d’un service d’hematologie, par l... more La presence de medicaments cytostatique a ete evaluee en sortie d’un service d’hematologie, par l’analyse des effets cyto et genotoxiques des effluents. Une quantification chimique est en cours. Cette caracterisation a servi pour mettre au point un effluent modele de ce type de pollution. Grace au fonctionnement en parallele de deux bioreacteurs a membrane, l’un traitant une eau domestique classique, l ‘autre traitant l’eau modele contenant les molecules cytostatiques, la pertinence de la technologie bioreacteur a membrane a ete demontree dans le cas d’une traitement d’une telle pollution : la presence de telles molecules aux effets biologiques semble en effet entrainer une reaction de protection de la part des microorganismes epurateurs, induisant une diminution de leur capacites de separation d’avec l’eau traitee. La presence des membranes est alors toute indiquee. Cette etude permet de demonter la faisabilite de bioreacteur a membrane dans le cas d’un pollution qui entraine des p...
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Faisabilite d’un bioreacteur a membrane pour la degradation de la toxicite d’un medicament antica... more Faisabilite d’un bioreacteur a membrane pour la degradation de la toxicite d’un medicament anticancereux.
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Journal of Applied Animal Research, 2021
To identify the specific biomarkers of exposure of DON in chickens, a toxicokinetic study was per... more To identify the specific biomarkers of exposure of DON in chickens, a toxicokinetic study was performed via oral or intravenous application of deoxynivalenol (DON). Doses of 0.75 and 2.25 mg DON/kg...
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The rejection of cyclophosphamide (CP) by nanofiltration (NF) and reverse osmosis (RO) membranes ... more The rejection of cyclophosphamide (CP) by nanofiltration (NF) and reverse osmosis (RO) membranes from ultrapure (Milli-Q) water and membrane bioreactor (MBR) effluent was investigated. Experimental results showed that the RO membrane provided excellent rejection (>90%) under all operating conditions. Conversely, efficiency of CP rejection by NF membrane was poor: in the range of 20-40% from Milli-Q water and around 60% from MBR effluent. Trans-membrane pressure, initial CP concentration and ionic strength of the feed solution had almost no effect on CP retention by NF. On the other hand, the water matrix proved to have a great influence: CP rejection rate by NF was clearly enhanced when MBR effluent was used as the background solution. Membrane fouling and interactions between the CP molecule and water matrix appeared to contribute to the higher rejection of CP.
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L'ochratoxine A (OTA) est une mycotoxine nephrotoxique, produite par plusieurs especes de cha... more L'ochratoxine A (OTA) est une mycotoxine nephrotoxique, produite par plusieurs especes de champignons, des genres Aspergillus et Penicillium, se developpant en particulier sur les denrees alimentaires au cours de leur stockage. L'exposition de l'homme a cette toxine se fait via la chaine alimentaire aussi bien par les produits vegetaux que par la viande. Chez les animaux, elle est clairement cancerogene au niveau du rein, chez l'homme elle a ete classee par le CIRC en 1993 (Centre international de la recherche sur le cancer) comme "potentiellement cancerogene pour l'homme" (groupe 2B). Le mecanisme par lequel cette toxine induit des cancers n’est pas totalement elucide et fait l’objet de debats. En effet, il reste a determiner si c'est un cancerogene genotoxique (via une liaison covalente sur l'ADN) ou un cancerogene epigenetique (non genotoxique, via un stress oxydatif par exemple). Le but de notre travail est de clarifier le mecanisme d’action...
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Toxins
Deoxynivalenol (DON), a trichothecene mycotoxin produced by Fusarium species, is the most widespr... more Deoxynivalenol (DON), a trichothecene mycotoxin produced by Fusarium species, is the most widespread mycotoxin in poultry feed worldwide. Long term-exposure from low to moderate DON concentrations can produce alteration in growth performance and impairment of the health status of birds. To evaluate the efficacy of mycotoxin-detoxifying agent alleviating the toxic effects of DON, the most relevant biomarkers of toxicity of DON in chickens should be firstly determined. The specific biomarker of exposure of DON in chickens is DON-3 sulphate found in different biological matrices (plasma and excreta). Regarding the nonspecific biomarkers called also biomarkers of effect, the most relevant ones are the impairment of the productive parameters, the intestinal morphology (reduction of villus height) and the enlargement of the gizzard. Moreover, the biomarkers of effect related to physiology (decrease of blood proteins, triglycerides, hemoglobin, erythrocytes, and lymphocytes and the increas...
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Animals
The present study with 1-day-old male broilers (Ross 308) was conducted to evaluate the effects o... more The present study with 1-day-old male broilers (Ross 308) was conducted to evaluate the effects of deoxynivalenol (DON) at different levels (5 and 15 mg/kg feed) on growth performance, relative weight of organs, morphology of the small intestine, serum biochemistry, and welfare parameters of broiler chickens. Forty-five broiler chicks were randomly divided into three different experimental groups with five replicates each: (1) control group received a non-contaminated diet, (2) contaminated diet with 5 mg DON/kg of feed, and (3) contaminated diet with 15 mg DON/kg of feed for 42 days. Results showed that feed artificially contaminated with DON at guidance level (5 mg/kg diet) did not affect growth performance parameters. However, 15 mg/kg reduced body weight gain and altered feed efficiency. DON at two assayed levels significantly increased the absolute and relative weight of thymus and the relative weight of gizzard and decreased the absolute and the relative weight of the colon. C...
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Foodborne Pathogens and Disease
Yeast cell wall (YCW) products are currently used as substitutes to antibiotic growth promoters, ... more Yeast cell wall (YCW) products are currently used as substitutes to antibiotic growth promoters, to improve animal performances, and to reduce the incidence of infectious diseases in livestock. They are claimed to bind enteropathogens, thus interfering with their colonization in the intestinal mucosa. Although the anti-infectious activity of YCW products on Gram-positive pathogens like Clostridium perfringens has been reported in vivo, in vitro evidences on the adsorption of C. perfringens by YCW fractions are not yet available. Preliminary results showed that purified YCW products exert antimicrobial activity toward C. perfringens. Using the adsorption isotherm approach, we measured the ability of YCW products in adsorbing C. perfringens, thus reducing its viability. Dosages of YCW products >1 mg/mL adsorbed 4 Log colony-forming unit (CFU)/mL of C. perfringens in buffered solution. The maximum adsorption of the bacterium was reached in 3 h, whereas only one product of four YCW products retained the adsorption up to 6 h. The analysis of equilibrium isotherms and adsorption kinetics revealed that all products adsorb C. perfringens in a dose- and time-dependent manner, with high affinity and capacity, sequestering up to 4 Log CFU/mg of product. The determination of adsorption parameters allows to differentiate among adsorbents and select the most efficient product. This approach discriminated among YCW products more efficiently than the antimicrobial assay. In conclusion, this study suggests that the ability of YCW products in reducing C. perfringens viability can be the result of an adsorption mechanism.
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Papers by virginie Marquis