The fungal cell wall is an essential organelle required for maintaining cell integrity and also p... more The fungal cell wall is an essential organelle required for maintaining cell integrity and also plays an important role in the primary interactions between pathogenic fungi and their hosts. PGA13 encodes a GPI protein in the human pathogen Candida albicans, which is highly up-regulated during cell wall regeneration in protoplasts. The Pga13 protein contains a unique tandem repeat, which is present five times and is characterized by conserved spacing between the four cysteine residues. Furthermore, the mature protein contains 38% serine and threonine residues, and therefore probably is a highly glycosylated cell wall protein. Consistent with this, a chimeric Pga13-V5 protein could be localized to the cell wall, but only after deglycosylation was performed. Disruption of PGA13 led to increased sensitivity to Congo red, Calcofluor white, and zymolyase, and to a diminished ability of protoplasts to recover their cell wall. In addition, pga13Δ mutants exhibited delayed filamentation, a higher surface hydrophobicity, and increased adherence and flocculation (cell-cell interactions). Furthermore, transcript profiling showed that expression of four members of the ALS family (adhesin-encoding genes) is up-regulated in the pga13Δ null mutant. Altogether, these results indicate that Pga13 is a wall-localized protein that contributes to cell wall synthesis and is important for acquiring normal surface properties. The contribution of Pga13 to surface hydrophilicity may be important for cell dispersal during development of invasive infections, and possibly for morphological development. This is consistent with the observed reduced virulence of pga13Δ mutants in a mouse model of disseminated candidiasis.
... of Computers Science and Artificial Intelligence ETSI Informática, University of Granada 1807... more ... of Computers Science and Artificial Intelligence ETSI Informática, University of Granada 18071, Granada, SPAIN {L. Castillo, Faro, Oscar, Palao}@ decsai. ugr. ... In this case a user-friendly in-put/output from/to GIS or project management and moni-toring is strongly required. ...
... Paediatrics hematopoietic stem cell transplantation: experience in 10 years Gustavo Dufort y ... more ... Paediatrics hematopoietic stem cell transplantation: experience in 10 years Gustavo Dufort y Alvarez1, Mariela Castiglioni1, Carolina Pagés1, Agustín Dabezies1, Jorge Decaro2, Luis Castillo3 ... In alloge-nic HSCT acute graft versus host disease was the leading cause of death. ...
The fungal cell wall is an essential organelle required for maintaining cell integrity and also p... more The fungal cell wall is an essential organelle required for maintaining cell integrity and also plays an important role in the primary interactions between pathogenic fungi and their hosts. PGA13 encodes a GPI protein in the human pathogen Candida albicans, which is highly up-regulated during cell wall regeneration in protoplasts. The Pga13 protein contains a unique tandem repeat, which is present five times and is characterized by conserved spacing between the four cysteine residues. Furthermore, the mature protein contains 38% serine and threonine residues, and therefore probably is a highly glycosylated cell wall protein. Consistent with this, a chimeric Pga13-V5 protein could be localized to the cell wall, but only after deglycosylation was performed. Disruption of PGA13 led to increased sensitivity to Congo red, Calcofluor white, and zymolyase, and to a diminished ability of protoplasts to recover their cell wall. In addition, pga13Δ mutants exhibited delayed filamentation, a higher surface hydrophobicity, and increased adherence and flocculation (cell-cell interactions). Furthermore, transcript profiling showed that expression of four members of the ALS family (adhesin-encoding genes) is up-regulated in the pga13Δ null mutant. Altogether, these results indicate that Pga13 is a wall-localized protein that contributes to cell wall synthesis and is important for acquiring normal surface properties. The contribution of Pga13 to surface hydrophilicity may be important for cell dispersal during development of invasive infections, and possibly for morphological development. This is consistent with the observed reduced virulence of pga13Δ mutants in a mouse model of disseminated candidiasis.
... of Computers Science and Artificial Intelligence ETSI Informática, University of Granada 1807... more ... of Computers Science and Artificial Intelligence ETSI Informática, University of Granada 18071, Granada, SPAIN {L. Castillo, Faro, Oscar, Palao}@ decsai. ugr. ... In this case a user-friendly in-put/output from/to GIS or project management and moni-toring is strongly required. ...
... Paediatrics hematopoietic stem cell transplantation: experience in 10 years Gustavo Dufort y ... more ... Paediatrics hematopoietic stem cell transplantation: experience in 10 years Gustavo Dufort y Alvarez1, Mariela Castiglioni1, Carolina Pagés1, Agustín Dabezies1, Jorge Decaro2, Luis Castillo3 ... In alloge-nic HSCT acute graft versus host disease was the leading cause of death. ...
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