Me ZiaUllah from Pakistan. I have done MSc in Entomology from the University of Agriculture Faisalabad, Pakistan. I have a specialty in Public Health Pest Surveillance and Control.
The studies were conducted to determine the efficacy of four insecticides, imidacloprid (Confidor... more The studies were conducted to determine the efficacy of four insecticides, imidacloprid (Confidor 200 SL) @ 150 ml/acre, acetamiprid (Mospilan 20 SP) @ 80g/acre, carbosulfan (Advantage 20 EC) @ 300 ml/acre and thiamethoxam (Actara 25 WP) @ 24g/acre with different mode of actions were tested against mustard aphid (Lipaphis erysimi Kalt.) in the field of canola crop at Entomological Research Area of University of Agriculture Faisalabad during 2013-14. The data was recorded pre and post (24 hrs, 48 hrs, 72 hrs and 1 week) treatment. Results showed that all the four insecticides, viz., imidacloprid (Confidor 200 SL) @ 150 ml/acre, acetamiprid (Mospilan 20 SP) @ 80 g/acre, carbosulfan (Advantage 20 EC) @ 300 ml/acre and thiamethoxam (Actara 25 WP) @ 24 g/acre, Advantage 20 EC (carbosulfan) @ 300 ml/acre gave satisfactory results as compared with control. But statistically highest population reduction was found with the application of Advantage 20 EC (carbosulfan) @ 300 ml/acre while the ...
Under the laboratory conditions (28 C±1, 70 ± 5%% relative humidity) a trial was conducted in the... more Under the laboratory conditions (28 C±1, 70 ± 5%% relative humidity) a trial was conducted in the laboratory of Department of Entomology, University of Agriculture Faisalabad during 2012-13 to evaluate the effect of comparative toxicity of different insecticides against adults of the ladybird beetle (Coccinella septempunctata L.). In this experiment seven treatments used namely Ememectin Benzoate (Timer® 1.9 EC), Indoxacarb (Steward® 150 EC), Imidachloprid (Crown® 70 WS), Chlorpyrifos (Helmat® 40 EC), Spinosad (Tracer® 480 SC), Leufenoron (Track® 10 EC) and Acetamaprid (Astarp® 250 WP). The experiment was laid out with five replications under Completely Randomized Design (CRD). Leaf dip bioassay method was used for Coccinella septempunctata L. Probit analysis was applied for statistical analysis. Our Results showed that the most toxic chemical was Chlorpyrifos that caused 100% mortality of adults of ladybird beetle after 72 hours and imidachloprid was the safest insecticide which ca...
Breeding preference of dengue vectors in Pakistan: An entomological investigation, 2019
Dengue fever (DF) and dengue hemorrhagic fever (DHF) are considered important reemerging arbovira... more Dengue fever (DF) and dengue hemorrhagic fever (DHF) are considered important reemerging arboviral diseases in more than 100 tropical and subtropical countries of the world. The Entomological surveillance of Dengue vector was carried out in six highly populated urban areas of Rawalpindi as per the WHO procedure. A total of 492 houses were searched for Aedes breeding and 262 houses found positive. About 731 water containers were searched, out of which 281 found positive for Aedes. Breeding preference ratio (BPR) was also calculated and discussed. The overall house index (HI), container index (CI), breteau index (BI) were 53.25, 38.44, and 57.11 respectively. Breeding Preference Ratio (BPR) was observed to be highest in water coolers (2.16), Discarded tyres (1.71), underground cement tank (1.69) and air condition water outlet containers (1.56). The results revealed that all the study areas of Rawalpindi city were found to positive for Ae. aegypti and Ae. albobictus.
Under the laboratory conditions (28 o C±1, 70 ± 5%% relative humidity) a trial was conducted in t... more Under the laboratory conditions (28 o C±1, 70 ± 5%% relative humidity) a trial was conducted in the laboratory of Department of Entomology, University of Agriculture Faisalabad during 2012-13 to evaluate the effect of comparative toxicity of different insecticides against adults of the ladybird beetle (Coccinella septempunctata L.). In this experiment seven treatments used namely Ememectin Benzoate (Timer® 1.9 EC), Indoxacarb (Steward® 150 EC), Imidachloprid (Crown® 70 WS), Chlorpyrifos (Helmat® 40 EC), Spinosad (Tracer® 480 SC), Leufenoron (Track® 10 EC) and Acetamaprid (Astarp® 250 WP). The experiment was laid out with five replications under Completely Randomized Design (CRD). Leaf dip bioassay method was used for Coccinella septempunctata L. Probit analysis was applied for statistical analysis. Our Results showed that the most toxic chemical was Chlorpyrifos that caused 100% mortality of adults of ladybird beetle after 72 hours and imidachloprid was the safest insecticide which can be included in integrated pest management practices. The order of toxicity on the basis of mortality was Chlorpyrifos > Indoxacarb > Spinosad > Acetamaprid > Ememectin Benzoate > Leufenoron > Imidachloprid.
The studies were conducted to determine the efficacy of four insecticides, imidacloprid (Confidor... more The studies were conducted to determine the efficacy of four insecticides, imidacloprid (Confidor 200 SL) @ 150 ml/acre, acetamiprid (Mospilan 20 SP) @ 80g/acre, carbosulfan (Advantage 20 EC) @ 300 ml/acre and thiamethoxam (Actara 25 WP) @ 24g/acre with different mode of actions were tested against mustard aphid (Lipaphis erysimi Kalt.) in the field of canola crop at Entomological Research Area of University of Agriculture Faisalabad during 2013-14. The data was recorded pre and post (24 hrs, 48 hrs, 72 hrs and 1 week) treatment. Results showed that all the four insecticides, viz., imidacloprid (Confidor 200 SL) @ 150 ml/acre, acetamiprid (Mospilan 20 SP) @ 80 g/acre, carbosulfan (Advantage 20 EC) @ 300 ml/acre and thiamethoxam (Actara 25 WP) @ 24 g/acre, Advantage 20 EC (carbosulfan) @ 300 ml/acre gave satisfactory results as compared with control. But statistically highest population reduction was found with the application of Advantage 20 EC (carbosulfan) @ 300 ml/acre while the lowest population reduction was found with Mospilan 20 SP (acetamiprid) @ 80 g/acre. It is concluded that all insecticides were effective statistically against the Lipaphis erysimi (Kalt.) need to be tested on different cultivars of rapeseed mustard.
Dengue is crucial vector-borne viral human disease across the tropical and subtropical region of ... more Dengue is crucial vector-borne viral human disease across the tropical and subtropical region of world. Dengue is transmitted from one person to another person by biting of female Aedes aegypti and Aedes albopictus. Dengue is present in every corner of the world (WHO), almost 128 countries are known to dengue outbreak, about 100 billion dengue cases are reported worldwide and more than 390 million humans with chance of infection annually. Pakistan is subtropical country and main hotspot for vector-borne diseases such as dengue haemorrhagic fever, malaria, leishmaniasis, Crimean-cango and West Nile virus diseases. Four dengue serotypes are present in Pakistan and circulating whole year with peak outbreak between (September-November) during post monsoon periods. In Pakistan dengue epidemic is a major public threat since 2005, following millions of people at risk, till 2016 almost 71649 dengue cases are reported with 757 deaths. There are several factors such as climatic change, urbanization, travel, socioeconomic activity, miscommunication and shortage of surveillance. Worst dengue outbreak occurred in Pakistan during 2011 with 21685 dengue reported cases (350 deaths). Current literature was reviewed, stressing on epidemiology, vector distribution (different region) of country, pathogenesis and future strategies for control. The paper review assesses the epidemiology of Aedes vector capacity linked dengue threats and outbreaks public health burden and losses in different geographical settings in Pakistan. Introduction Dengue is vector borne viral disease of 21 st Century and is regarded as a threat to public health results in heavy socioeconomic encumbrance on a large number of tropical, subtropical and temperate region of world [1, 2]. Currently it is estimated 50 % of world population is living in dengue prone areas where they are in danger of dengue arboviral disease and 50% of world population live in dengue indigenous countries [2, 3]. Approximately 100 million dengue cases are reported and more than 390 million people with chance of infection worldwide each year [4]. The causal agent of dengue virus (DENV) belong to family Flaviviridae and genus Flavivirus, four anti genetically distinct serotype are (DENV 1, DENV 2, DENV 3, DENV 4) and five recognized serotype (DENV 5) characterized by neutralization assays [5, 6]. These virus are transferred by female Aedes mosquito (Diptera: Culicidae) especially Aedes aegypti (Linnaeus) and lesser extend Aedes albopictus (Skuse) that feed on human blood both indoors and outdoors during dawn to dusk and can be found in tropical and subtropical region particularly dominant in urban environment and spreading out to rural areas. These mosquitoes can also transfer chikungunya, Zika infection and yellow fever [7, 8]. Dengue is complex disease with clinical illness is traditional classified ranging from mild to severe form including undifferentiated fever, dengue fever (DF), dengue hemorrhagic fever (DHF), dengue shock syndrome (DSS) and extended dengue syndrome. DF is a terrible, flu-like disease that distresses babies, young children, adults and causes rarely death. Dengue should be doubted with high fever (40 °C) followed by these signs: severe headache, joint and muscle discomfort, swollen glands nausea, pain behind the eyes and vomiting. After 4-10 days bite from an infected mosquito symptoms generally appeared and last for 2-7 days. However DHF and DSS are more severe and possibly lethal forms with symptoms like plasma leaking, respiratory distress, severe abdominal pain, fluid accumulation, severe bleeding, or organ injury.
Mosquito is a vector of different diseases like Malaria, Filariasis and Dengue fever. It has been... more Mosquito is a vector of different diseases like Malaria, Filariasis and Dengue fever. It has been increasing development of resistance to synthetic chemicals, so plant extracts may be the alternate source of mosquito control agent. This study was conducted on five different plant extracts as Nicotiana tabacum, Ocimum basilicum, Datura stramonium, Chenopodium album and Cassia fistula against Culex quinquefasciatus Say. to evaluate them as an efficient larvicide potential. Plant extracts offered a virtually untapped chemical compounds with different potential uses. Study was focused on laboratory determination of LC50 and LC90 values by a bioassay test. These plant extracts were tested on 3 rd instar of mosquito larvae. The plant extracts were applied with different concentrations as 400, 200, 100, 50, 25 ppm with acetone as control and mortality was counted after 24, 48 and 72 hours. The efficiency of plant extracts was recorded in term of % age mortality. From these extracts Cassia fistula gave 100% mortality and Nicotiana tabacum gave 98% mortality after 72 hours of application. In sense of LC50 and LC90 values Cassia fistula showed 50.272ppm and 203.994ppm respectively. LC50 and LC90 values of Nicotiana tabacum were 17.772ppm and 206.485ppm respectively.
Comparative toxicity of different insecticides including flubendiamide (480 SC), chlorantranilipr... more Comparative toxicity of different insecticides including flubendiamide (480 SC), chlorantraniliprole (2.0 SC), chlorpyrifos (40 EC), imidachloprid (200 SL), bifenthrin (100 EC), spinetoram (120 SC) and spinosad (240SC) were tested on all larval instars and adults of Chrysoperla carnea in Laboratory of University of Agriculture Faisalabad, Pakistan during 2012-13 at 27± 2 o C and 65 ± 5% R.H. under Completely Randomized Design. Data was recorded after 24 and 48 hours of exposure to the insecticides. Chlorpyrifos was the most toxic insecticide caused 100%, 96.67%, 93.33%,86.67% mortality of 1 st , 2 nd , 3 rd larval instars and adults respectively after 48 hours while spinosad, flubendiamide and bifenthrin are somewhat safer than the rest of insecticides. Chlorantraniliprole showed the least LC50 values for all instars and adults of C. carnea whereas flubendiamide indicated the highest LC50 values for all larval instars after 48 hours. In case of adults imidachloprid showed the highest LC50 values after 48 hours. All Insecticides showed harmful effect to C. carnea but some of them cause less damage, may be included in the Integrated Pest Management (IPM) rogram with somewhat adverse residual effect on bio-control agents.
The studies were conducted to determine the efficacy of four insecticides, imidacloprid (Confidor... more The studies were conducted to determine the efficacy of four insecticides, imidacloprid (Confidor 200 SL) @ 150 ml/acre, acetamiprid (Mospilan 20 SP) @ 80g/acre, carbosulfan (Advantage 20 EC) @ 300 ml/acre and thiamethoxam (Actara 25 WP) @ 24g/acre with different mode of actions were tested against mustard aphid (Lipaphis erysimi Kalt.) in the field of canola crop at Entomological Research Area of University of Agriculture Faisalabad during 2013-14. The data was recorded pre and post (24 hrs, 48 hrs, 72 hrs and 1 week) treatment. Results showed that all the four insecticides, viz., imidacloprid (Confidor 200 SL) @ 150 ml/acre, acetamiprid (Mospilan 20 SP) @ 80 g/acre, carbosulfan (Advantage 20 EC) @ 300 ml/acre and thiamethoxam (Actara 25 WP) @ 24 g/acre, Advantage 20 EC (carbosulfan) @ 300 ml/acre gave satisfactory results as compared with control. But statistically highest population reduction was found with the application of Advantage 20 EC (carbosulfan) @ 300 ml/acre while the ...
Under the laboratory conditions (28 C±1, 70 ± 5%% relative humidity) a trial was conducted in the... more Under the laboratory conditions (28 C±1, 70 ± 5%% relative humidity) a trial was conducted in the laboratory of Department of Entomology, University of Agriculture Faisalabad during 2012-13 to evaluate the effect of comparative toxicity of different insecticides against adults of the ladybird beetle (Coccinella septempunctata L.). In this experiment seven treatments used namely Ememectin Benzoate (Timer® 1.9 EC), Indoxacarb (Steward® 150 EC), Imidachloprid (Crown® 70 WS), Chlorpyrifos (Helmat® 40 EC), Spinosad (Tracer® 480 SC), Leufenoron (Track® 10 EC) and Acetamaprid (Astarp® 250 WP). The experiment was laid out with five replications under Completely Randomized Design (CRD). Leaf dip bioassay method was used for Coccinella septempunctata L. Probit analysis was applied for statistical analysis. Our Results showed that the most toxic chemical was Chlorpyrifos that caused 100% mortality of adults of ladybird beetle after 72 hours and imidachloprid was the safest insecticide which ca...
Breeding preference of dengue vectors in Pakistan: An entomological investigation, 2019
Dengue fever (DF) and dengue hemorrhagic fever (DHF) are considered important reemerging arbovira... more Dengue fever (DF) and dengue hemorrhagic fever (DHF) are considered important reemerging arboviral diseases in more than 100 tropical and subtropical countries of the world. The Entomological surveillance of Dengue vector was carried out in six highly populated urban areas of Rawalpindi as per the WHO procedure. A total of 492 houses were searched for Aedes breeding and 262 houses found positive. About 731 water containers were searched, out of which 281 found positive for Aedes. Breeding preference ratio (BPR) was also calculated and discussed. The overall house index (HI), container index (CI), breteau index (BI) were 53.25, 38.44, and 57.11 respectively. Breeding Preference Ratio (BPR) was observed to be highest in water coolers (2.16), Discarded tyres (1.71), underground cement tank (1.69) and air condition water outlet containers (1.56). The results revealed that all the study areas of Rawalpindi city were found to positive for Ae. aegypti and Ae. albobictus.
Under the laboratory conditions (28 o C±1, 70 ± 5%% relative humidity) a trial was conducted in t... more Under the laboratory conditions (28 o C±1, 70 ± 5%% relative humidity) a trial was conducted in the laboratory of Department of Entomology, University of Agriculture Faisalabad during 2012-13 to evaluate the effect of comparative toxicity of different insecticides against adults of the ladybird beetle (Coccinella septempunctata L.). In this experiment seven treatments used namely Ememectin Benzoate (Timer® 1.9 EC), Indoxacarb (Steward® 150 EC), Imidachloprid (Crown® 70 WS), Chlorpyrifos (Helmat® 40 EC), Spinosad (Tracer® 480 SC), Leufenoron (Track® 10 EC) and Acetamaprid (Astarp® 250 WP). The experiment was laid out with five replications under Completely Randomized Design (CRD). Leaf dip bioassay method was used for Coccinella septempunctata L. Probit analysis was applied for statistical analysis. Our Results showed that the most toxic chemical was Chlorpyrifos that caused 100% mortality of adults of ladybird beetle after 72 hours and imidachloprid was the safest insecticide which can be included in integrated pest management practices. The order of toxicity on the basis of mortality was Chlorpyrifos > Indoxacarb > Spinosad > Acetamaprid > Ememectin Benzoate > Leufenoron > Imidachloprid.
The studies were conducted to determine the efficacy of four insecticides, imidacloprid (Confidor... more The studies were conducted to determine the efficacy of four insecticides, imidacloprid (Confidor 200 SL) @ 150 ml/acre, acetamiprid (Mospilan 20 SP) @ 80g/acre, carbosulfan (Advantage 20 EC) @ 300 ml/acre and thiamethoxam (Actara 25 WP) @ 24g/acre with different mode of actions were tested against mustard aphid (Lipaphis erysimi Kalt.) in the field of canola crop at Entomological Research Area of University of Agriculture Faisalabad during 2013-14. The data was recorded pre and post (24 hrs, 48 hrs, 72 hrs and 1 week) treatment. Results showed that all the four insecticides, viz., imidacloprid (Confidor 200 SL) @ 150 ml/acre, acetamiprid (Mospilan 20 SP) @ 80 g/acre, carbosulfan (Advantage 20 EC) @ 300 ml/acre and thiamethoxam (Actara 25 WP) @ 24 g/acre, Advantage 20 EC (carbosulfan) @ 300 ml/acre gave satisfactory results as compared with control. But statistically highest population reduction was found with the application of Advantage 20 EC (carbosulfan) @ 300 ml/acre while the lowest population reduction was found with Mospilan 20 SP (acetamiprid) @ 80 g/acre. It is concluded that all insecticides were effective statistically against the Lipaphis erysimi (Kalt.) need to be tested on different cultivars of rapeseed mustard.
Dengue is crucial vector-borne viral human disease across the tropical and subtropical region of ... more Dengue is crucial vector-borne viral human disease across the tropical and subtropical region of world. Dengue is transmitted from one person to another person by biting of female Aedes aegypti and Aedes albopictus. Dengue is present in every corner of the world (WHO), almost 128 countries are known to dengue outbreak, about 100 billion dengue cases are reported worldwide and more than 390 million humans with chance of infection annually. Pakistan is subtropical country and main hotspot for vector-borne diseases such as dengue haemorrhagic fever, malaria, leishmaniasis, Crimean-cango and West Nile virus diseases. Four dengue serotypes are present in Pakistan and circulating whole year with peak outbreak between (September-November) during post monsoon periods. In Pakistan dengue epidemic is a major public threat since 2005, following millions of people at risk, till 2016 almost 71649 dengue cases are reported with 757 deaths. There are several factors such as climatic change, urbanization, travel, socioeconomic activity, miscommunication and shortage of surveillance. Worst dengue outbreak occurred in Pakistan during 2011 with 21685 dengue reported cases (350 deaths). Current literature was reviewed, stressing on epidemiology, vector distribution (different region) of country, pathogenesis and future strategies for control. The paper review assesses the epidemiology of Aedes vector capacity linked dengue threats and outbreaks public health burden and losses in different geographical settings in Pakistan. Introduction Dengue is vector borne viral disease of 21 st Century and is regarded as a threat to public health results in heavy socioeconomic encumbrance on a large number of tropical, subtropical and temperate region of world [1, 2]. Currently it is estimated 50 % of world population is living in dengue prone areas where they are in danger of dengue arboviral disease and 50% of world population live in dengue indigenous countries [2, 3]. Approximately 100 million dengue cases are reported and more than 390 million people with chance of infection worldwide each year [4]. The causal agent of dengue virus (DENV) belong to family Flaviviridae and genus Flavivirus, four anti genetically distinct serotype are (DENV 1, DENV 2, DENV 3, DENV 4) and five recognized serotype (DENV 5) characterized by neutralization assays [5, 6]. These virus are transferred by female Aedes mosquito (Diptera: Culicidae) especially Aedes aegypti (Linnaeus) and lesser extend Aedes albopictus (Skuse) that feed on human blood both indoors and outdoors during dawn to dusk and can be found in tropical and subtropical region particularly dominant in urban environment and spreading out to rural areas. These mosquitoes can also transfer chikungunya, Zika infection and yellow fever [7, 8]. Dengue is complex disease with clinical illness is traditional classified ranging from mild to severe form including undifferentiated fever, dengue fever (DF), dengue hemorrhagic fever (DHF), dengue shock syndrome (DSS) and extended dengue syndrome. DF is a terrible, flu-like disease that distresses babies, young children, adults and causes rarely death. Dengue should be doubted with high fever (40 °C) followed by these signs: severe headache, joint and muscle discomfort, swollen glands nausea, pain behind the eyes and vomiting. After 4-10 days bite from an infected mosquito symptoms generally appeared and last for 2-7 days. However DHF and DSS are more severe and possibly lethal forms with symptoms like plasma leaking, respiratory distress, severe abdominal pain, fluid accumulation, severe bleeding, or organ injury.
Mosquito is a vector of different diseases like Malaria, Filariasis and Dengue fever. It has been... more Mosquito is a vector of different diseases like Malaria, Filariasis and Dengue fever. It has been increasing development of resistance to synthetic chemicals, so plant extracts may be the alternate source of mosquito control agent. This study was conducted on five different plant extracts as Nicotiana tabacum, Ocimum basilicum, Datura stramonium, Chenopodium album and Cassia fistula against Culex quinquefasciatus Say. to evaluate them as an efficient larvicide potential. Plant extracts offered a virtually untapped chemical compounds with different potential uses. Study was focused on laboratory determination of LC50 and LC90 values by a bioassay test. These plant extracts were tested on 3 rd instar of mosquito larvae. The plant extracts were applied with different concentrations as 400, 200, 100, 50, 25 ppm with acetone as control and mortality was counted after 24, 48 and 72 hours. The efficiency of plant extracts was recorded in term of % age mortality. From these extracts Cassia fistula gave 100% mortality and Nicotiana tabacum gave 98% mortality after 72 hours of application. In sense of LC50 and LC90 values Cassia fistula showed 50.272ppm and 203.994ppm respectively. LC50 and LC90 values of Nicotiana tabacum were 17.772ppm and 206.485ppm respectively.
Comparative toxicity of different insecticides including flubendiamide (480 SC), chlorantranilipr... more Comparative toxicity of different insecticides including flubendiamide (480 SC), chlorantraniliprole (2.0 SC), chlorpyrifos (40 EC), imidachloprid (200 SL), bifenthrin (100 EC), spinetoram (120 SC) and spinosad (240SC) were tested on all larval instars and adults of Chrysoperla carnea in Laboratory of University of Agriculture Faisalabad, Pakistan during 2012-13 at 27± 2 o C and 65 ± 5% R.H. under Completely Randomized Design. Data was recorded after 24 and 48 hours of exposure to the insecticides. Chlorpyrifos was the most toxic insecticide caused 100%, 96.67%, 93.33%,86.67% mortality of 1 st , 2 nd , 3 rd larval instars and adults respectively after 48 hours while spinosad, flubendiamide and bifenthrin are somewhat safer than the rest of insecticides. Chlorantraniliprole showed the least LC50 values for all instars and adults of C. carnea whereas flubendiamide indicated the highest LC50 values for all larval instars after 48 hours. In case of adults imidachloprid showed the highest LC50 values after 48 hours. All Insecticides showed harmful effect to C. carnea but some of them cause less damage, may be included in the Integrated Pest Management (IPM) rogram with somewhat adverse residual effect on bio-control agents.
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