The aim of this study was to synthesize and characterize polymeric porous scaffolds associated wi... more The aim of this study was to synthesize and characterize polymeric porous scaffolds associated with different calcium phosphates (CaP) and Mesenchymal Stem Cells (MSC) for regenerative dentistry application. Chitosan-Xanthan Scaffolds (CX) were associated with 5% of the two CaP types, Hydroxyapatite (HA) and Brushite (BS). For advanced cell therapies, the scaffolds were associated with MSC. The scaffold structures were characterized by X Ray Diffraction (DRX), Fourier Transformed Infrared (FTIR), Thermogravimetric Analysis (TGA), Scanning Electron Microscopy analysis (SEM) and the compressive strength. The in vitro Cytotoxicity was performed and the in vivo Biocompatibility by histomorphometry and inflammatory cells number count. XRD showed the amorphous phase of CX and main peaks of the CaP phases in the HA and BS scaffolds. FTIR showed the amide I and II bands, characteristic of Chitosan, and carboxyl group, characteristic of Xanthan. PO4 bands were found in CaP scaffolds. SEM showed pores and CaP fillers incorporated and adhered to the polymer in the CX-HA, CX-BS, CX-HA + MSC and CX-BS + MSC. Compressive strength and Modulus of Elasticity analysis exhibited higher values for CX-BS scaffolds, followed by CX-HA and CX. All scaffolds showed acceptable cells viability after 24 h and 48 h; however, the CX scaffolds showed higher cell viability in 48 h. CX-BS produced significantly higher inflammatory cells number after 7 and 30 days of implantation. After 60 days of implantation, CX + MSC and CX-HA + MSC showed the lowest inflammatory cells number. The CaP improved the mechanical properties of scaffolds but decreased the cell viability. MSCs improved the inflammatory response after 60 days.
Bioactive bioceramics are a family of osteoconductive materials that include bioactive glass and ... more Bioactive bioceramics are a family of osteoconductive materials that include bioactive glass and calcium phosphates such as hydroxyapatite (HA) and tricalcium phosphate (β-TCP or α-TCP). These materials exhibit excellent bone binding properties and are usually designed to be used as bone fillers or as bioactive coatings on metal implants. Their in vivo resorption ability may be modulated by controlling synthesis and processing parameters. Bioceramics can be associated to stem cells in cell-based therapies, using transplanted cells to guide the spatially complex process of tissue formation, thus optimizing osteoinduction, osteoconduction, and osteogenesis. Although tissue engineering strategies using stem cells aim to ameliorate the prognosis of the grafted materials, there are several questions still to be addressed, such as which are the best materials and which are the best adult stem cells to be associated with these scaffolds in order to improve and hasten the replacement of bon...
The aim of this study was to synthesize and characterize polymeric porous scaffolds associated wi... more The aim of this study was to synthesize and characterize polymeric porous scaffolds associated with different calcium phosphates (CaP) and Mesenchymal Stem Cells (MSC) for regenerative dentistry application. Chitosan-Xanthan Scaffolds (CX) were associated with 5% of the two CaP types, Hydroxyapatite (HA) and Brushite (BS). For advanced cell therapies, the scaffolds were associated with MSC. The scaffold structures were characterized by X Ray Diffraction (DRX), Fourier Transformed Infrared (FTIR), Thermogravimetric Analysis (TGA), Scanning Electron Microscopy analysis (SEM) and the compressive strength. The in vitro Cytotoxicity was performed and the in vivo Biocompatibility by histomorphometry and inflammatory cells number count. XRD showed the amorphous phase of CX and main peaks of the CaP phases in the HA and BS scaffolds. FTIR showed the amide I and II bands, characteristic of Chitosan, and carboxyl group, characteristic of Xanthan. PO4 bands were found in CaP scaffolds. SEM showed pores and CaP fillers incorporated and adhered to the polymer in the CX-HA, CX-BS, CX-HA + MSC and CX-BS + MSC. Compressive strength and Modulus of Elasticity analysis exhibited higher values for CX-BS scaffolds, followed by CX-HA and CX. All scaffolds showed acceptable cells viability after 24 h and 48 h; however, the CX scaffolds showed higher cell viability in 48 h. CX-BS produced significantly higher inflammatory cells number after 7 and 30 days of implantation. After 60 days of implantation, CX + MSC and CX-HA + MSC showed the lowest inflammatory cells number. The CaP improved the mechanical properties of scaffolds but decreased the cell viability. MSCs improved the inflammatory response after 60 days.
Bioactive bioceramics are a family of osteoconductive materials that include bioactive glass and ... more Bioactive bioceramics are a family of osteoconductive materials that include bioactive glass and calcium phosphates such as hydroxyapatite (HA) and tricalcium phosphate (β-TCP or α-TCP). These materials exhibit excellent bone binding properties and are usually designed to be used as bone fillers or as bioactive coatings on metal implants. Their in vivo resorption ability may be modulated by controlling synthesis and processing parameters. Bioceramics can be associated to stem cells in cell-based therapies, using transplanted cells to guide the spatially complex process of tissue formation, thus optimizing osteoinduction, osteoconduction, and osteogenesis. Although tissue engineering strategies using stem cells aim to ameliorate the prognosis of the grafted materials, there are several questions still to be addressed, such as which are the best materials and which are the best adult stem cells to be associated with these scaffolds in order to improve and hasten the replacement of bon...
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Papers by Rafael Barbosa