The application of tissue culture techniques in woody plants offers a valuable alternative for th... more The application of tissue culture techniques in woody plants offers a valuable alternative for the propagation of elite trees. With the objective of obtaining the in vitro establishment of mahogany (Swietenia macrophylla King), the starting material were young buds taken from plants in field conditions. For the disinfection of the vegetal material two concentrations of hypochlorite of sodium (NaClO) combined with three times of exposition were studied. The percentage of the variables contaminated explants, necrotic explants and survival, were evaluated. Later, the method of disinfection of the explants was determined. The nodules segments and apical buds were cultivated under environmental conditions of controlled light and temperature, in a culture medium of MS with reduction of nitrates to the half and supplemented with five concentrations of cytokinin (6-BAP). In all the tests forty repetitions by treatment were made and one explant by essay tube with 10 milliliters of culture me...
In sorghum [Sorghum bicolor (L.) Moench], developed protocols for plant regeneration via somatic ... more In sorghum [Sorghum bicolor (L.) Moench], developed protocols for plant regeneration via somatic embryogenesis do not include maturation stage. The present work was carried out with the aim of achieving the maturation and germination of sorghum somatic embryos in cultivar 'CIAP 132R-05'. It were studied four concentrations of sucrose (30, 50, 70 and 90 g l-1), two of abscisic acid (0.25 and 0.5 μM) and a control without this growth regulator. Germination initiation (days) and number of somatic embryos with complete germination were evaluated in three periods (1 - 7, 8 - 14 and 15 - 21 days of culture). In addition, the effect of 6-BAP (8.9, 17.8 and 26.6 μM) on somatic embryo germination was determined. The germination start time (days) and after 21 days the number of somatic embryos with complete germination and plants with malformations were determined. The addition of 70 g l-1 sucrose in the culture medium without abscisic acid increased the germination of the somatic emb...
Common bean (Phaseolus vulgaris L.) is an important food legume worldwide. Salinity induces losse... more Common bean (Phaseolus vulgaris L.) is an important food legume worldwide. Salinity induces losses in common bean yield, particularly in arid, semiarid and i...
The common bean ( Phaseolus vulgaris L.) production has been very important in Cuba, howeve... more The common bean ( Phaseolus vulgaris L.) production has been very important in Cuba, however, several factors affect the yield of this crop and not all cultivars of beans respond the same way to the stressful conditions. The aim of this study was to determine the effect of water deficit induced by PEG 6000 on the cultivar ‘ICA Pijao’. The results showed that increasing concentrations of PEG 6000, reduced the percentage of P. vulgaris cv. ‘ICA Pijao’ in vitro germination, it confirmed that this stressor agent could be useful to elucidate the physiological response and PEG 6000 concentration to be used in bioassays to evaluate the plant response in this cultivar should be less than 18%. Key words: common beans, drought, in vitro selection
Drought during the terminal phase of the crop is a global limitation for the production of common... more Drought during the terminal phase of the crop is a global limitation for the production of common beans ( Phaseolus vulgaris L.) and it is a problem that will be aggravated by the effects of climate change. The objective of this work was to select morphological indicators for hydric stress response in P. vulgaris in greenhouse. Four lines of brown grain and the commercial cultivar ‘BAT-93’ were evaluated. A water stress treatment was established where the irrigation was suspended in the pre-flowering stage until the harvest and a control with irrigation throughout the all crop cycle. Ten plants were random selected at each treatment and the angle of the basal roots, main root length (cm), the number of legumes/plant, the number of grains/legumes and the weight of 100 grains (g) were evaluated. Root length was a morphological indicator of the plants response to water stress under these conditions. It allowed to differentiate the response of the lines and cultivar ‘BAT-93’. The rest o...
The production of common bean ( Phaseolus vulgaris L.) is limited by diverse stressful conditions... more The production of common bean ( Phaseolus vulgaris L.) is limited by diverse stressful conditions, amongst them high temperatures. Therefore, obtaining tolerant bean cultivars without affecting their yield is a purpose of the breeding programs of this important legume. Evaluation of physiological variables to determine the response of cultivars of P. vulgaris under heat stress can be a significant contribution to the identification of tolerant cultivars. This work was performed in order to determine the response of ten P. vulgaris cultivars under induced heat stress conditions. The effect of temperature on electrolytes leakage and release of inorganic phosphate in leaf tissue of P. vulgaris was determinate. In all tested temperatures (35-60 ° C), there was an increase at electrolytes leakage, regarding the value of this variable at 25 ° C for 10 cultivars under study. Significant differences of electrolytes leakage between cultivars were presented in the treatments where leaf tissue...
The identification of Phaseolus vulgaris L. cultivars capable to tolerate abiotic stress without ... more The identification of Phaseolus vulgaris L. cultivars capable to tolerate abiotic stress without affecting its yield is a purpose of the breeding programs of this legume. The objective of this work was to differentiate P. vulgaris cultivars by the foliar tissue response exposed to hydric and saline stress in vitro induced. Leaf discs of 'Cuba Cueto 25-9 Negro' cultivar were placed in Petri dishes on filter paper. On each plate 2 ml of 6, 8, 10, 12, 14, 16 and 18% (w / v) PEG 6000 or 50, 100, 150, 200, 250, and 300 mM NaCl solution were added to induce hydric or saline stress. Deionized water was used as the control. The plates were maintained for 48 h at 25 ± 2 ° C. A scale of degrees of affectation was elaborated according to the percentage of foliar area with necrosis. In ten cultivars of P. vulgaris the assay was applied and its response recorded by the elaborated scale. Leaf tissue at 48 h showed necrotic areas. The designed scale was structured with seven grades where g...
The objective of this study was to characterize, under greenhouse conditions, a population of 2 0... more The objective of this study was to characterize, under greenhouse conditions, a population of 2 000 plants of Anthurium andraeanum variety ‘Lambada’ regenerated from somatic embryos and suckers. Temperatures of 29±2oC, a relative humidity of 80% and a brightness of 60-70% were maintained during the period of culture in greenhouses. Bigger values of the variables evaluated in leaves, flower and plants were shown in plants regenerated from somatic embryos. Longitude of spate in plants coming from suckers was bigger but differences were not significant among the two populations under greenhouse conditions. Plants off-type were not observed in plants obtained from somatic embryos and growth was similar to plants obtainet from suckers. Key words: Araceae , greenhouse, production, somatic embryo, suckers
Genotype, type of explant and concentration of growth regulators have been studied to increase ef... more Genotype, type of explant and concentration of growth regulators have been studied to increase efficiency in plant regeneration of Phaseolus vulgaris L. However, studies on the effect of the gelling agent in shoots induction are not enough. Therefore, the present study aimed to determine the effect of gelling agent on organogenic calli proliferation and shoots regeneration of P. vulgaris cv. `BAT-93', `BAT-304', `BAT-482', `Ica Pijao' and `CIAP 7247F'. Callus fresh weight (g) was determined. Number of calli with shoots, number of shoots per callus and number of hyperhydric shoots was measured in shoots regeneration. Results showed that gelling influenced the calli multiplication and shoot regeneration in the five cultivars. `Ica Pijao', `CIAP7247F' and `BAT-93' showed the highest values in calli fresh weight (g) using microbiological agar E. Besides, `BAT-482' reached the highest value of fresh weight of calli in culture medium hardened with gelrite. `BAT-304' calli showed similar values of fresh weight in both treatments. During regeneration, cultivars `BAT-93' and `CIAP 7247F' showed the highest values in the number of callus with shoots and shoots per callus in culture medium solidified with gelrite. Nevertheless, in this treatment there were more hyperhydric shoots regardless the cultivar. Keywords: agar, in vitro culture, common bean, gelrite.
Titulo en ingles: Influence of light type and intensity on induction and proliferation of somatic... more Titulo en ingles: Influence of light type and intensity on induction and proliferation of somatic embryos in soybean Resumen: La embriogenesis somatica representa una alternativa para la regeneracion de plantas, sobre la cual influyen diversos factores durante el cultivo in vitro . Entre estos, las condiciones de iluminacion durante la formacion de embriones somaticos es determinante . Sin embargo, no existen trabajos que refieran el efecto de la luz solar en la formacion de embriones somaticos de soya. E ste trabajo tuvo como objetivo evaluar el efecto del tipo e intensidad de luz en la formacion y multiplicacion de embriones somaticos de soya cultivar INCASoy-27. Para ello, cotiledones inmaduros de 3,0-4,0 mm de longitud fueron colocados en medio de cultivo con 40 mg.l -1 de acido 2,4-diclorofenoxiacetico, sacarosa 3% y pH 7,0 en camaras de crecimiento con luz artificial e intensidades luminosas de 5-10 µmol.m -2 .s -1 y 68-73 µmol.m -2 .s -1 , y en camaras de crecimiento con luz ...
Stress caused by drought affects food crops, such as common bean ( Phaseolus vulgaris L.). The ob... more Stress caused by drought affects food crops, such as common bean ( Phaseolus vulgaris L.). The objective of this work was to determine the response in the greenhouse of black grain common beans lines in stress conditions for irrigation to pre-flowering. Four possible black grain mutant lines were evaluated supporting two treatments: application of irrigation considering the water requirements of the crop and the second, application of irrigation to pre-flowering. The sowing and the attention to the crop were carried out, with the indications established in the technical instructions. Ten plants were randomly selected in each treatment and evaluated: length of the roots/plants (cm), number of legumes/plant, number of grains/legumes and weight of 100 seeds (g). As a result, the lines showed the highest values of root length in the conditions of irrigation application to pre-flowering. In the rest of the variables evaluated, the lines showed a similar response in two treatments. This r...
Genetic breeding in Phaseolus by genetic transformation requires an efficient selection system. T... more Genetic breeding in Phaseolus by genetic transformation requires an efficient selection system. The present investigation was aimed to determine the minimum lethal concentration of glufosinate-ammonium (Finale ®) in beans plants cv. `CIAP 7247F' grown in greenhouse. Different concentrations of the herbicide were applied to the foliage of plants in acclimatization phase (20, 30 y 40 mg l -1 ) and the control. Results showed that the minimum lethal concentration in plants in acclimatization phase was 30 mg l -1 . Results also demonstrated that is possible the use of the herbicide as a selective agent of beans transformants cv. `CIAP 7247F' carrying the bar gene. Keywords: genetic transformation, herbicide, selective agent, tissue culture
This work was carried out with the objective to form somatic embryos of sorghum, cv. ‘CIAP 132R-0... more This work was carried out with the objective to form somatic embryos of sorghum, cv. ‘CIAP 132R-05’ starting from callus obtained from sections of in vitro shoots. For the formation of callus, different concentrations of 2,4-D were studied as well as three concentrations of ascorbic acid to eliminate the phenolic oxidation. To increase the percentage of callus formation with embryogenic structures, different segments of the shoots were used. For the formation of somatic embryos, different concentrations of 2,4-D; 6-BAP and L-Proline were added to the culture media. The greatest callus formation (50%) was obtained in the culture medium with 18.1 µM of 2,4-D. When 50 mg l -1 of ascorbic acid was added to the culture medium, the percentage of callus formation increased to 67.5%, and was couple with absence of oxidation of the medium and the explant. The frequency of callus formation with embryogenic structures increased to 95% with the use of segment 1 of the shoot sections in vitro a...
RESUMEN Las leguminosas son una fuente de proteína muy importante en la dieta tanto humana como a... more RESUMEN Las leguminosas son una fuente de proteína muy importante en la dieta tanto humana como animal y de estas las del género Phaseolus son las más empleadas en la nutrición. La mayoría de los sistemas de transformación genética descritos hasta el momento requieren seleccionar las plantas que contengan el transgen introducido mediante un marcador de selección. La utilización de uno u otro, requiere que primero se determinen las concentraciones adecuadas de este y su efecto inhibitorio en el crecimiento de los explantes. Por lo tanto, el objetivo del presente trabajo fue determinar la concentración mínima inhibitoria de los antibióticos Geneticina G-418 e Higromicina B que inhibieran el crecimiento de explantes de Phaseolus vulgaris var. CIAP 72-47. Como material vegetal se utilizaron segmentos de tallo de 5-6 mm de longitud que contenían el nudo cotiledonal y habían sido obtenidos a partir de semillas maduras. Para la selección se empleó el medio de cultivo de inducción de brotes...
Genotype, type of explant and concentration of growth regulators have been studied to increase ef... more Genotype, type of explant and concentration of growth regulators have been studied to increase efficiency in plant regeneration of Phaseolus vulgaris L. However, studies on the effect of the gelling agent in shoots induction are not enough. Therefore, the present study aimed to determine the effect of gelling agent on organogenic calli proliferation and shoots regeneration of P. vulgaris cv. `BAT-93', `BAT-304', `BAT-482', `Ica Pijao' and `CIAP 7247F'. Callus fresh weight (g) was determined. Number of calli with shoots, number of shoots per callus and number of hyperhydric shoots was measured in shoots regeneration. Results showed that gelling influenced the calli multiplication and shoot regeneration in the five cultivars. `Ica Pijao', `CIAP7247F' and `BAT-93' showed the highest values in calli fresh weight (g) using microbiological agar E. Besides, `BAT-482' reached the highest value of fresh weight of calli in culture medium hardened with gelri...
The genetic improvement of guava helped by biotechnological techniques has been based on the micr... more The genetic improvement of guava helped by biotechnological techniques has been based on the micropropagation of plantlet sections of botanical seed, high yielding materials, with the purpose of cloning each individual, so that constituting genetically uniform in vitro plant lines that are possibly promissory. In the present work a comparison was made, under field conditions, between micropropagated lines selected from cv. EEA 18-40, and its parents propagated by cutting and graft with respect to yield. The evaluated variables were: fruit number per plant, fruit weight and yield. The best results were presented by the clonal lines A-33 and A-9, with 22.8 and 18.8 ton.ha-1 respectively in the third cycle of production. Concerning genetic improvement and seed production, the lines selected were A-9 and A-33, for presenting higher yields, fruit weight and fruit number per plant than cutting and graft control. The vegetable material obtained by micropropagation selected allowed to incre...
The application of tissue culture techniques in woody plants offers a valuable alternative for th... more The application of tissue culture techniques in woody plants offers a valuable alternative for the propagation of elite trees. With the objective of obtaining the in vitro establishment of mahogany (Swietenia macrophylla King), the starting material were young buds taken from plants in field conditions. For the disinfection of the vegetal material two concentrations of hypochlorite of sodium (NaClO) combined with three times of exposition were studied. The percentage of the variables contaminated explants, necrotic explants and survival, were evaluated. Later, the method of disinfection of the explants was determined. The nodules segments and apical buds were cultivated under environmental conditions of controlled light and temperature, in a culture medium of MS with reduction of nitrates to the half and supplemented with five concentrations of cytokinin (6-BAP). In all the tests forty repetitions by treatment were made and one explant by essay tube with 10 milliliters of culture me...
In sorghum [Sorghum bicolor (L.) Moench], developed protocols for plant regeneration via somatic ... more In sorghum [Sorghum bicolor (L.) Moench], developed protocols for plant regeneration via somatic embryogenesis do not include maturation stage. The present work was carried out with the aim of achieving the maturation and germination of sorghum somatic embryos in cultivar 'CIAP 132R-05'. It were studied four concentrations of sucrose (30, 50, 70 and 90 g l-1), two of abscisic acid (0.25 and 0.5 μM) and a control without this growth regulator. Germination initiation (days) and number of somatic embryos with complete germination were evaluated in three periods (1 - 7, 8 - 14 and 15 - 21 days of culture). In addition, the effect of 6-BAP (8.9, 17.8 and 26.6 μM) on somatic embryo germination was determined. The germination start time (days) and after 21 days the number of somatic embryos with complete germination and plants with malformations were determined. The addition of 70 g l-1 sucrose in the culture medium without abscisic acid increased the germination of the somatic emb...
Common bean (Phaseolus vulgaris L.) is an important food legume worldwide. Salinity induces losse... more Common bean (Phaseolus vulgaris L.) is an important food legume worldwide. Salinity induces losses in common bean yield, particularly in arid, semiarid and i...
The common bean ( Phaseolus vulgaris L.) production has been very important in Cuba, howeve... more The common bean ( Phaseolus vulgaris L.) production has been very important in Cuba, however, several factors affect the yield of this crop and not all cultivars of beans respond the same way to the stressful conditions. The aim of this study was to determine the effect of water deficit induced by PEG 6000 on the cultivar ‘ICA Pijao’. The results showed that increasing concentrations of PEG 6000, reduced the percentage of P. vulgaris cv. ‘ICA Pijao’ in vitro germination, it confirmed that this stressor agent could be useful to elucidate the physiological response and PEG 6000 concentration to be used in bioassays to evaluate the plant response in this cultivar should be less than 18%. Key words: common beans, drought, in vitro selection
Drought during the terminal phase of the crop is a global limitation for the production of common... more Drought during the terminal phase of the crop is a global limitation for the production of common beans ( Phaseolus vulgaris L.) and it is a problem that will be aggravated by the effects of climate change. The objective of this work was to select morphological indicators for hydric stress response in P. vulgaris in greenhouse. Four lines of brown grain and the commercial cultivar ‘BAT-93’ were evaluated. A water stress treatment was established where the irrigation was suspended in the pre-flowering stage until the harvest and a control with irrigation throughout the all crop cycle. Ten plants were random selected at each treatment and the angle of the basal roots, main root length (cm), the number of legumes/plant, the number of grains/legumes and the weight of 100 grains (g) were evaluated. Root length was a morphological indicator of the plants response to water stress under these conditions. It allowed to differentiate the response of the lines and cultivar ‘BAT-93’. The rest o...
The production of common bean ( Phaseolus vulgaris L.) is limited by diverse stressful conditions... more The production of common bean ( Phaseolus vulgaris L.) is limited by diverse stressful conditions, amongst them high temperatures. Therefore, obtaining tolerant bean cultivars without affecting their yield is a purpose of the breeding programs of this important legume. Evaluation of physiological variables to determine the response of cultivars of P. vulgaris under heat stress can be a significant contribution to the identification of tolerant cultivars. This work was performed in order to determine the response of ten P. vulgaris cultivars under induced heat stress conditions. The effect of temperature on electrolytes leakage and release of inorganic phosphate in leaf tissue of P. vulgaris was determinate. In all tested temperatures (35-60 ° C), there was an increase at electrolytes leakage, regarding the value of this variable at 25 ° C for 10 cultivars under study. Significant differences of electrolytes leakage between cultivars were presented in the treatments where leaf tissue...
The identification of Phaseolus vulgaris L. cultivars capable to tolerate abiotic stress without ... more The identification of Phaseolus vulgaris L. cultivars capable to tolerate abiotic stress without affecting its yield is a purpose of the breeding programs of this legume. The objective of this work was to differentiate P. vulgaris cultivars by the foliar tissue response exposed to hydric and saline stress in vitro induced. Leaf discs of 'Cuba Cueto 25-9 Negro' cultivar were placed in Petri dishes on filter paper. On each plate 2 ml of 6, 8, 10, 12, 14, 16 and 18% (w / v) PEG 6000 or 50, 100, 150, 200, 250, and 300 mM NaCl solution were added to induce hydric or saline stress. Deionized water was used as the control. The plates were maintained for 48 h at 25 ± 2 ° C. A scale of degrees of affectation was elaborated according to the percentage of foliar area with necrosis. In ten cultivars of P. vulgaris the assay was applied and its response recorded by the elaborated scale. Leaf tissue at 48 h showed necrotic areas. The designed scale was structured with seven grades where g...
The objective of this study was to characterize, under greenhouse conditions, a population of 2 0... more The objective of this study was to characterize, under greenhouse conditions, a population of 2 000 plants of Anthurium andraeanum variety ‘Lambada’ regenerated from somatic embryos and suckers. Temperatures of 29±2oC, a relative humidity of 80% and a brightness of 60-70% were maintained during the period of culture in greenhouses. Bigger values of the variables evaluated in leaves, flower and plants were shown in plants regenerated from somatic embryos. Longitude of spate in plants coming from suckers was bigger but differences were not significant among the two populations under greenhouse conditions. Plants off-type were not observed in plants obtained from somatic embryos and growth was similar to plants obtainet from suckers. Key words: Araceae , greenhouse, production, somatic embryo, suckers
Genotype, type of explant and concentration of growth regulators have been studied to increase ef... more Genotype, type of explant and concentration of growth regulators have been studied to increase efficiency in plant regeneration of Phaseolus vulgaris L. However, studies on the effect of the gelling agent in shoots induction are not enough. Therefore, the present study aimed to determine the effect of gelling agent on organogenic calli proliferation and shoots regeneration of P. vulgaris cv. `BAT-93', `BAT-304', `BAT-482', `Ica Pijao' and `CIAP 7247F'. Callus fresh weight (g) was determined. Number of calli with shoots, number of shoots per callus and number of hyperhydric shoots was measured in shoots regeneration. Results showed that gelling influenced the calli multiplication and shoot regeneration in the five cultivars. `Ica Pijao', `CIAP7247F' and `BAT-93' showed the highest values in calli fresh weight (g) using microbiological agar E. Besides, `BAT-482' reached the highest value of fresh weight of calli in culture medium hardened with gelrite. `BAT-304' calli showed similar values of fresh weight in both treatments. During regeneration, cultivars `BAT-93' and `CIAP 7247F' showed the highest values in the number of callus with shoots and shoots per callus in culture medium solidified with gelrite. Nevertheless, in this treatment there were more hyperhydric shoots regardless the cultivar. Keywords: agar, in vitro culture, common bean, gelrite.
Titulo en ingles: Influence of light type and intensity on induction and proliferation of somatic... more Titulo en ingles: Influence of light type and intensity on induction and proliferation of somatic embryos in soybean Resumen: La embriogenesis somatica representa una alternativa para la regeneracion de plantas, sobre la cual influyen diversos factores durante el cultivo in vitro . Entre estos, las condiciones de iluminacion durante la formacion de embriones somaticos es determinante . Sin embargo, no existen trabajos que refieran el efecto de la luz solar en la formacion de embriones somaticos de soya. E ste trabajo tuvo como objetivo evaluar el efecto del tipo e intensidad de luz en la formacion y multiplicacion de embriones somaticos de soya cultivar INCASoy-27. Para ello, cotiledones inmaduros de 3,0-4,0 mm de longitud fueron colocados en medio de cultivo con 40 mg.l -1 de acido 2,4-diclorofenoxiacetico, sacarosa 3% y pH 7,0 en camaras de crecimiento con luz artificial e intensidades luminosas de 5-10 µmol.m -2 .s -1 y 68-73 µmol.m -2 .s -1 , y en camaras de crecimiento con luz ...
Stress caused by drought affects food crops, such as common bean ( Phaseolus vulgaris L.). The ob... more Stress caused by drought affects food crops, such as common bean ( Phaseolus vulgaris L.). The objective of this work was to determine the response in the greenhouse of black grain common beans lines in stress conditions for irrigation to pre-flowering. Four possible black grain mutant lines were evaluated supporting two treatments: application of irrigation considering the water requirements of the crop and the second, application of irrigation to pre-flowering. The sowing and the attention to the crop were carried out, with the indications established in the technical instructions. Ten plants were randomly selected in each treatment and evaluated: length of the roots/plants (cm), number of legumes/plant, number of grains/legumes and weight of 100 seeds (g). As a result, the lines showed the highest values of root length in the conditions of irrigation application to pre-flowering. In the rest of the variables evaluated, the lines showed a similar response in two treatments. This r...
Genetic breeding in Phaseolus by genetic transformation requires an efficient selection system. T... more Genetic breeding in Phaseolus by genetic transformation requires an efficient selection system. The present investigation was aimed to determine the minimum lethal concentration of glufosinate-ammonium (Finale ®) in beans plants cv. `CIAP 7247F' grown in greenhouse. Different concentrations of the herbicide were applied to the foliage of plants in acclimatization phase (20, 30 y 40 mg l -1 ) and the control. Results showed that the minimum lethal concentration in plants in acclimatization phase was 30 mg l -1 . Results also demonstrated that is possible the use of the herbicide as a selective agent of beans transformants cv. `CIAP 7247F' carrying the bar gene. Keywords: genetic transformation, herbicide, selective agent, tissue culture
This work was carried out with the objective to form somatic embryos of sorghum, cv. ‘CIAP 132R-0... more This work was carried out with the objective to form somatic embryos of sorghum, cv. ‘CIAP 132R-05’ starting from callus obtained from sections of in vitro shoots. For the formation of callus, different concentrations of 2,4-D were studied as well as three concentrations of ascorbic acid to eliminate the phenolic oxidation. To increase the percentage of callus formation with embryogenic structures, different segments of the shoots were used. For the formation of somatic embryos, different concentrations of 2,4-D; 6-BAP and L-Proline were added to the culture media. The greatest callus formation (50%) was obtained in the culture medium with 18.1 µM of 2,4-D. When 50 mg l -1 of ascorbic acid was added to the culture medium, the percentage of callus formation increased to 67.5%, and was couple with absence of oxidation of the medium and the explant. The frequency of callus formation with embryogenic structures increased to 95% with the use of segment 1 of the shoot sections in vitro a...
RESUMEN Las leguminosas son una fuente de proteína muy importante en la dieta tanto humana como a... more RESUMEN Las leguminosas son una fuente de proteína muy importante en la dieta tanto humana como animal y de estas las del género Phaseolus son las más empleadas en la nutrición. La mayoría de los sistemas de transformación genética descritos hasta el momento requieren seleccionar las plantas que contengan el transgen introducido mediante un marcador de selección. La utilización de uno u otro, requiere que primero se determinen las concentraciones adecuadas de este y su efecto inhibitorio en el crecimiento de los explantes. Por lo tanto, el objetivo del presente trabajo fue determinar la concentración mínima inhibitoria de los antibióticos Geneticina G-418 e Higromicina B que inhibieran el crecimiento de explantes de Phaseolus vulgaris var. CIAP 72-47. Como material vegetal se utilizaron segmentos de tallo de 5-6 mm de longitud que contenían el nudo cotiledonal y habían sido obtenidos a partir de semillas maduras. Para la selección se empleó el medio de cultivo de inducción de brotes...
Genotype, type of explant and concentration of growth regulators have been studied to increase ef... more Genotype, type of explant and concentration of growth regulators have been studied to increase efficiency in plant regeneration of Phaseolus vulgaris L. However, studies on the effect of the gelling agent in shoots induction are not enough. Therefore, the present study aimed to determine the effect of gelling agent on organogenic calli proliferation and shoots regeneration of P. vulgaris cv. `BAT-93', `BAT-304', `BAT-482', `Ica Pijao' and `CIAP 7247F'. Callus fresh weight (g) was determined. Number of calli with shoots, number of shoots per callus and number of hyperhydric shoots was measured in shoots regeneration. Results showed that gelling influenced the calli multiplication and shoot regeneration in the five cultivars. `Ica Pijao', `CIAP7247F' and `BAT-93' showed the highest values in calli fresh weight (g) using microbiological agar E. Besides, `BAT-482' reached the highest value of fresh weight of calli in culture medium hardened with gelri...
The genetic improvement of guava helped by biotechnological techniques has been based on the micr... more The genetic improvement of guava helped by biotechnological techniques has been based on the micropropagation of plantlet sections of botanical seed, high yielding materials, with the purpose of cloning each individual, so that constituting genetically uniform in vitro plant lines that are possibly promissory. In the present work a comparison was made, under field conditions, between micropropagated lines selected from cv. EEA 18-40, and its parents propagated by cutting and graft with respect to yield. The evaluated variables were: fruit number per plant, fruit weight and yield. The best results were presented by the clonal lines A-33 and A-9, with 22.8 and 18.8 ton.ha-1 respectively in the third cycle of production. Concerning genetic improvement and seed production, the lines selected were A-9 and A-33, for presenting higher yields, fruit weight and fruit number per plant than cutting and graft control. The vegetable material obtained by micropropagation selected allowed to incre...
Uploads
Papers by Raul Collado