Abstract The green synthesis of nanoparticles from bioactive compounds have attracted a wide rang... more Abstract The green synthesis of nanoparticles from bioactive compounds have attracted a wide range of application, due to increased drug efficacy and less toxicity in the nanosized mediated drug delivery model. In this study, we have fabricated copper nanoparticles (CuNPs) from the fruits of Prunus nepalensis (P. nepalensis) extract. Therefore, the aim of present study was to investigate the anticancer ability of P. nepalensis fruit phytochemical copper nanoaprticles (PNFPCuNP) on cancerous human breast cell line (MCF-7) and healthy (MCFA10) cell lines. Crystalline CuNPs of P. nepalensis synthesis was confirmed by different physicochemical analytical techniques such as UV–Visible Spectroscopy (UV–Vis); Fourier-Transform Infrared Spectroscopy (FT-IR); Scanning Electron Microscopy (SEM); and Transmission Electron Microscopy (TEM). Nanoparticle Size was found to be ranging from 35 to 50 nm with the average size of 42.5 nm. Further, after synthesized compounds were tested anticancer activity on human breast cancer cell lines. Following 72 h treatment to PNFPCuNP, the expression of apoptotic marker genes (P21, p53, P14/P19, Caspase-3) were studied in MCF-7 cells treated at 100 to 200 μg of PNFP-CuNP. Our results showed that PNFP-CuNP increased the gene expression of apoptotic genes in a dose-dependent manner. The real-time PCR data showed a significant upregulation in p53, Bax, caspase-3, and caspase-9 and down regulation in the mRNA expression of Ras and Myc genes in MCF-7 cells exposed to PNFP-CuNP. Collectively, the data from this study stated that P. nepalennsis fruit extract phytochemical derived nanoparticles induced apoptosis via the up regulation of tumour suppressor genes and down regulation of oncogenes in MCF-7 cells. Finally, our study confirmed the CuNPs synthesis from P. nepalensis fruit phytochemical, which showed environmental friendly anticancer activity.
International Journal of Pharmacy and Pharmaceutical Sciences, 2015
Objective: To investigate the interaction of LfcinB (Lactoferricin B) with GSTP1 (Glutathione S-T... more Objective: To investigate the interaction of LfcinB (Lactoferricin B) with GSTP1 (Glutathione S-Transferase P1) from human placental and breast cancer cell line MDA-MB-231. Methods : We examined the interaction of Lfcin B with human placental GSTP1 and breast adenocarcinoma MD-MB-231 cell line. Enzyme activity of GSTP1 was measured with and without pre-incubation with Lfcin B. Kinetic variables were determined by incubating the enzyme reaction mixture with fixed GSH (reduced glutathione) concentration and varying CDNB (1-chloro-2, 4-dinitrobenzene) concentrations or fixed CDNB concentration and varying GSH concentrations. Results: Lfcin B is a competitive inhibitor with respect to GSH binding site (G site) and noncompetitive inhibitor with respect to hydrophobic substrate unit (H site) of human placental GSTP1 enzyme. Lfcin B was also incubated with GSTP1 from breast adenocarcinoma MDA-MB-231 cell line. The activity of GSTP1 was much higher (0.2665 μ mol/ml/min) in Lfcin B untreated...
Cancer is one of the most threatening diseases in the world which leads to death of the people. A... more Cancer is one of the most threatening diseases in the world which leads to death of the people. Although, several researches have been done for the diagnosis and treatment of cancer but its mechanism still remains unclear. Early detection of cancer is necessary as most of the tumors contain a large number of diseased cells which may have already metastasized. There are various diagnostic techniques such as medical imaging, tissue biopsy and ELISA (Enzyme-Linked Immunosorbent Assay) which are currently employed for diagnosing the cancer at an early-stage, but these diagnostic techniques are labor intensive, time consuming and expensive. So, here is very crucial condition for growth of cancer at an early-stage which is inexpensive and fast. The novel approach for the analysis of cancer nowadays is Quantum Dot (QD) detection system which is an easy, economical and rapid technology for cancer diagnosis. (QDs) are minute semiconductor small particles that have Opto - electronic property ...
Background: Slow stroking touch is generally perceived as pleasant and reduces thermal pain. Howe... more Background: Slow stroking touch is generally perceived as pleasant and reduces thermal pain. However, the tactile stimuli applied tend to be short-lasting and typically applied to the forearm. This study aimed to compare the effects of a long-lasting brushing stimulus applied to the facial region and the forearm on pressure pain thresholds (PPTs) taken on the hand. Outcome measurements were touch satiety and concurrent mechanical pain thresholds of the hand.Methods: A total of 24 participants were recruited and randomized to receive continuous stroking, utilizing a robotic stimulator, at C-tactile (CT) favorable (3 cm/s) and non-favorable (30 cm/s) velocities applied to the right face or forearm. Ratings of touch pleasantness and unpleasantness and PPTs from the hypothenar muscle of the right hand were collected at the start of stroking and once per minute for 5 min.Results: A reduction in PPTs (increased pain sensitivity) was observed over time (P < 0.001). However, the increase...
BACKGROUND The aim of this study was to synthesize and evaluate anticancer activity of 2-hydroxy ... more BACKGROUND The aim of this study was to synthesize and evaluate anticancer activity of 2-hydroxy benzaldehyde and 4-hydroxy benzaldehyde thiosemicarbazone (2-HBTSc and 4-HBTSc) against MCF-7 breast cancer cell line. MATERIALS AND METHODS The ligands were prepared and characterized by UV vis, IR and NMR. MTT assay was used to assess viability of cells. RNA isolation, extraction and cDNA synthesis were done. Then all groups were subjected to RT-qPCR using Gene expression specific primers. Also, western blot protein expression and molecular docking were done. Two-way ANOVA with Tukey post-hoc test was employed to test the significance using GraphPad Prism. RESULTS The IC50 values were 3.36 μg/ml and 3.60 μg/ml for 2-HBTSc and 4-HBTSc treated MCF-7 tumor cells respectively. Tumor cell growth inhibition ranged from 38 to 49.27% in 4-HBTSc treated cells, and 19 to 25% in 2-HBTSc treated cells with increase in doses 5 μg/ml to 20 μg/ml. The protein and gene expression result showed a significant upregulation in tumor suppressor and apoptosis inducing genes while, oncogene activity was significantly downregulated. Specifically, BRCA2 and pRB gene showed the highest expression in 4-HBTSc and 2-HBTSc treated cells respectively. Conversely, RAS oncogene was downregulated significantly. Docking result showed that both 2-HBTSc and 4-HBTSc have the potential to inhibit Estrogen Receptor Alpha Ligand Binding Domain, Human 17-Beta-hydroxysteroid dehydrogenase type 1 mutant protein and Human Topoisomerase II alpha that are expressed more during Breast Cancer. CONCLUSION The findings of this study imply that the test compound has potential for further study.
Acquired immunodeficiency syndrome (AIDS) has affected millions of people worldwide. The human im... more Acquired immunodeficiency syndrome (AIDS) has affected millions of people worldwide. The human immunodeficiency virus (HIV) which infects T cells by using CD4 as its main receptor. Currently different treatments are available against HIV infection which can improve life expectancy of the patient but still it remains incurable. CCR5, which is also required as a co-receptor by majority of HIV strains for entry into the target cells, is now being targeted for gene therapy to develop HIV resistance in patients. In this review, we discuss different strategies that are being adapted for CCR5-gene disruption in CD4+ T cells and in hematopoietic stem cells (HSCs) to generate a HIV-resistant immune system in infected individuals. If CCR5 gene that can shape HIV-resistant T cells, it will aim in new approaches in clinical trials. But these techniques have certain weaknesses and disadvantages, and will need to be paired with other strategies to form a full HIV remedy. There is also a need to establish methods to help deter HIV re-emergence following targeted CCR5 therapy. But ultimately, this brought us a better knowledge of the road to HIV treatment.
ABSTRACTBackgroundBile Salt Export Pump (BSEP/ABCB11) is important in the maintenance of the ente... more ABSTRACTBackgroundBile Salt Export Pump (BSEP/ABCB11) is important in the maintenance of the enterohepatic circulation of bile acids and drugs. Drugs such as rifampicin, glibenclamide inhibit BSEP. Progressive Familial Intrahepatic Cholestasis Type-2, a lethal pediatric disease, some forms of intrahepatic cholestasis of pregnancy, and drug-induced cholestasis are associated with BSEP dysfunction.MethodsWe started with a bioinformatic approach to identify the relationship between ABCB11 and other proteins, microRNAs, and drugs. Microarray data set of the liver samples from ABCB11 knockout mice was analyzed by GEO2R tool. Differentially expressed gene pathway enrichment analysis was done by ClueGo v2.5.5 app from Cytoscape. Protein-protein interaction network was constructed by STRING application in Cytoscape. Networks were analyzed using the Cytoscape software v3.7.1. CyTargetLinker v4.1.0 was used to screen the transcription factors, microRNAs and drugs. Predicted drugs were validat...
BackgroundGenomic safe harbors are sites in the genome which are safe for gene insertion such tha... more BackgroundGenomic safe harbors are sites in the genome which are safe for gene insertion such that the inserted gene will function properly, and the disruption of the genomic location doesn’t cause any foreseeable risk to the host. The AAVS1 site is the site which is disrupted upon integration of Adeno Associated Virus (AAV) and is considered a ‘safe-harbor’ in human genome because about one third of humans are infected with AAV and so far there is no apodictic evidence that AAV is pathogenic or disruption of AAVS1 causes any disease in man. Therefore, we chose to target AAVS1 site for the insertion of ABCB11, a bile acid transporter which is defective in Progressive Familial Intra Hepatic Cholestasis Type-2 (PFIC-2), a lethal disease of children where cytotoxic bile salts accumulate inside hepatocytes killing them and eventually the patient.MethodsWe used CRISPR Cas9 a genome editing tool to insert ABCB11 gene at AAVS1 site in human cell-lines.ResultsWe found that human ABCB11 sequ...
Background Quantitative sensory testing protocols for perceptions of pleasantness and unpleasantn... more Background Quantitative sensory testing protocols for perceptions of pleasantness and unpleasantness based on the German Research Network on Neuropathic Pain protocol were recently introduced. However, there are no reliability studies yet published. Aim To evaluate the intra-examiner (test-retest) and inter-examiner reliability for orofacial pleasantness and unpleasantness quantitative sensory testing protocols. Methods Sixteen healthy participants from Aarhus University (11 women and five men, mean age 24, range 21–26 years) contributed. Two examiners were trained in performing the entire quantitative sensory testing protocols for pleasantness and unpleasantness, which included the additional dynamic tactile stimulation test using a goat-hair brush. Each participant underwent examination of both protocols by each examiner (inter-examiner reliability) on day 1. They returned at least 8 days following the testing to be re-examined by one examiner (intra-examiner reliability). All tes...
Lactic acid bacteria (LAB)are the essential ingredients in probiotic foods, intestinal microflora... more Lactic acid bacteria (LAB)are the essential ingredients in probiotic foods, intestinal microflora, and dairy products able to cope up and exist in diverse environmental ranges. Samples were collected using sterile test tubes and transported to a laboratory in the icebox for further biochemical characterization. Gram test and catalase activity were examined after microscopically distinct colonies were sub-cultured to pure colonies based on standard gram and catalase test protocols. Subsequently, these bacteria were characterized for their ability to grow at various salt concentrations (5%,10%, and15%) and temperature gradients (15°C, 30°C, 45°C). Acid-tolerance was analyzed by growing the colonies in MRS broth adjusted to acidic pH (pH 3) and pH 7.2 (control). Bile tolerance of LAB isolates was assayed by growing in 0.3% bile-supplemented MRS agar. Bile salt hydrolase (BSH) activity was studied by growing 10 μl of the prepared overnight culture on BSH screening media containing MRS a...
Background/AimBreast cancer is one of the world’s leading cause of deaths in women. This study ev... more Background/AimBreast cancer is one of the world’s leading cause of deaths in women. This study evaluated the in-vitro anticancer activity of different thiosemicarbazones (HacTSc, HSTsc, 3-MBTSc, 4-NBTSc and 4-HBTSc) against MCF-7 human breast cancer cell line and MCF-10 normal cell.Materials and MethodsThe ligands were prepared and characterized by UV vis, IR,1H NMR. MTT assay was used to determine cell viability. Then data were analyzed using two-way ANOVA with Tukey post-hoc test. Result: The ligands have IC50value ranging from 2.271µg/ml to 7.081µg/ml. Acetone thiosemicarbazone and 3-Methoxybenzaldehyde thiosemicarbazone, were identified as the most potent against MCF-7 breast cancer cells with IC50value of 2.271µg/ml and 2.743µg/ml respectively. Whereas 4-Nitrobenzaldehyde thiosemicarbazone was the least potent. Also, the IC50of the normal MCF-10 cell indicated their activity were selective. Conclusion: The activity of the ligands were dose, position and substituents dependent. ...
International journal of biological macromolecules, Jan 15, 2018
Integrin-linked kinase (ILK) is a member of Ser/Thr kinase which interacts to the cytoplasmic dom... more Integrin-linked kinase (ILK) is a member of Ser/Thr kinase which interacts to the cytoplasmic domain of β-integrins, and thereby induces apoptosis. ILK is considered as potential drug target because it's direct involvement in the tumor progression. Here, we have performed molecular docking followed by 100 ns MD simulation to understand the mechanism of interaction of ILK with the ellagic acid (EA). EA is well known for its antiproliferative and antioxidant properties in cancer cell lines and animal models. We have observed that EA binds to the active site cavity of ILK and causes conformational changes in the ILK structure. The orientation of EA in the active pocket of ILK showed to have least RMSD values and stable. The average binding energy ILK-EA complex calculated during MMPBSA was -191.267 kJ/mol, indicating a relatively strong binding affinity. The actual binding affinity of EA to ILK was measured by fluorescence spectroscopy and K and n values were 9.28 μM and 1.9264 (~2...
Abstract The green synthesis of nanoparticles from bioactive compounds have attracted a wide rang... more Abstract The green synthesis of nanoparticles from bioactive compounds have attracted a wide range of application, due to increased drug efficacy and less toxicity in the nanosized mediated drug delivery model. In this study, we have fabricated copper nanoparticles (CuNPs) from the fruits of Prunus nepalensis (P. nepalensis) extract. Therefore, the aim of present study was to investigate the anticancer ability of P. nepalensis fruit phytochemical copper nanoaprticles (PNFPCuNP) on cancerous human breast cell line (MCF-7) and healthy (MCFA10) cell lines. Crystalline CuNPs of P. nepalensis synthesis was confirmed by different physicochemical analytical techniques such as UV–Visible Spectroscopy (UV–Vis); Fourier-Transform Infrared Spectroscopy (FT-IR); Scanning Electron Microscopy (SEM); and Transmission Electron Microscopy (TEM). Nanoparticle Size was found to be ranging from 35 to 50 nm with the average size of 42.5 nm. Further, after synthesized compounds were tested anticancer activity on human breast cancer cell lines. Following 72 h treatment to PNFPCuNP, the expression of apoptotic marker genes (P21, p53, P14/P19, Caspase-3) were studied in MCF-7 cells treated at 100 to 200 μg of PNFP-CuNP. Our results showed that PNFP-CuNP increased the gene expression of apoptotic genes in a dose-dependent manner. The real-time PCR data showed a significant upregulation in p53, Bax, caspase-3, and caspase-9 and down regulation in the mRNA expression of Ras and Myc genes in MCF-7 cells exposed to PNFP-CuNP. Collectively, the data from this study stated that P. nepalennsis fruit extract phytochemical derived nanoparticles induced apoptosis via the up regulation of tumour suppressor genes and down regulation of oncogenes in MCF-7 cells. Finally, our study confirmed the CuNPs synthesis from P. nepalensis fruit phytochemical, which showed environmental friendly anticancer activity.
International Journal of Pharmacy and Pharmaceutical Sciences, 2015
Objective: To investigate the interaction of LfcinB (Lactoferricin B) with GSTP1 (Glutathione S-T... more Objective: To investigate the interaction of LfcinB (Lactoferricin B) with GSTP1 (Glutathione S-Transferase P1) from human placental and breast cancer cell line MDA-MB-231. Methods : We examined the interaction of Lfcin B with human placental GSTP1 and breast adenocarcinoma MD-MB-231 cell line. Enzyme activity of GSTP1 was measured with and without pre-incubation with Lfcin B. Kinetic variables were determined by incubating the enzyme reaction mixture with fixed GSH (reduced glutathione) concentration and varying CDNB (1-chloro-2, 4-dinitrobenzene) concentrations or fixed CDNB concentration and varying GSH concentrations. Results: Lfcin B is a competitive inhibitor with respect to GSH binding site (G site) and noncompetitive inhibitor with respect to hydrophobic substrate unit (H site) of human placental GSTP1 enzyme. Lfcin B was also incubated with GSTP1 from breast adenocarcinoma MDA-MB-231 cell line. The activity of GSTP1 was much higher (0.2665 μ mol/ml/min) in Lfcin B untreated...
Cancer is one of the most threatening diseases in the world which leads to death of the people. A... more Cancer is one of the most threatening diseases in the world which leads to death of the people. Although, several researches have been done for the diagnosis and treatment of cancer but its mechanism still remains unclear. Early detection of cancer is necessary as most of the tumors contain a large number of diseased cells which may have already metastasized. There are various diagnostic techniques such as medical imaging, tissue biopsy and ELISA (Enzyme-Linked Immunosorbent Assay) which are currently employed for diagnosing the cancer at an early-stage, but these diagnostic techniques are labor intensive, time consuming and expensive. So, here is very crucial condition for growth of cancer at an early-stage which is inexpensive and fast. The novel approach for the analysis of cancer nowadays is Quantum Dot (QD) detection system which is an easy, economical and rapid technology for cancer diagnosis. (QDs) are minute semiconductor small particles that have Opto - electronic property ...
Background: Slow stroking touch is generally perceived as pleasant and reduces thermal pain. Howe... more Background: Slow stroking touch is generally perceived as pleasant and reduces thermal pain. However, the tactile stimuli applied tend to be short-lasting and typically applied to the forearm. This study aimed to compare the effects of a long-lasting brushing stimulus applied to the facial region and the forearm on pressure pain thresholds (PPTs) taken on the hand. Outcome measurements were touch satiety and concurrent mechanical pain thresholds of the hand.Methods: A total of 24 participants were recruited and randomized to receive continuous stroking, utilizing a robotic stimulator, at C-tactile (CT) favorable (3 cm/s) and non-favorable (30 cm/s) velocities applied to the right face or forearm. Ratings of touch pleasantness and unpleasantness and PPTs from the hypothenar muscle of the right hand were collected at the start of stroking and once per minute for 5 min.Results: A reduction in PPTs (increased pain sensitivity) was observed over time (P < 0.001). However, the increase...
BACKGROUND The aim of this study was to synthesize and evaluate anticancer activity of 2-hydroxy ... more BACKGROUND The aim of this study was to synthesize and evaluate anticancer activity of 2-hydroxy benzaldehyde and 4-hydroxy benzaldehyde thiosemicarbazone (2-HBTSc and 4-HBTSc) against MCF-7 breast cancer cell line. MATERIALS AND METHODS The ligands were prepared and characterized by UV vis, IR and NMR. MTT assay was used to assess viability of cells. RNA isolation, extraction and cDNA synthesis were done. Then all groups were subjected to RT-qPCR using Gene expression specific primers. Also, western blot protein expression and molecular docking were done. Two-way ANOVA with Tukey post-hoc test was employed to test the significance using GraphPad Prism. RESULTS The IC50 values were 3.36 μg/ml and 3.60 μg/ml for 2-HBTSc and 4-HBTSc treated MCF-7 tumor cells respectively. Tumor cell growth inhibition ranged from 38 to 49.27% in 4-HBTSc treated cells, and 19 to 25% in 2-HBTSc treated cells with increase in doses 5 μg/ml to 20 μg/ml. The protein and gene expression result showed a significant upregulation in tumor suppressor and apoptosis inducing genes while, oncogene activity was significantly downregulated. Specifically, BRCA2 and pRB gene showed the highest expression in 4-HBTSc and 2-HBTSc treated cells respectively. Conversely, RAS oncogene was downregulated significantly. Docking result showed that both 2-HBTSc and 4-HBTSc have the potential to inhibit Estrogen Receptor Alpha Ligand Binding Domain, Human 17-Beta-hydroxysteroid dehydrogenase type 1 mutant protein and Human Topoisomerase II alpha that are expressed more during Breast Cancer. CONCLUSION The findings of this study imply that the test compound has potential for further study.
Acquired immunodeficiency syndrome (AIDS) has affected millions of people worldwide. The human im... more Acquired immunodeficiency syndrome (AIDS) has affected millions of people worldwide. The human immunodeficiency virus (HIV) which infects T cells by using CD4 as its main receptor. Currently different treatments are available against HIV infection which can improve life expectancy of the patient but still it remains incurable. CCR5, which is also required as a co-receptor by majority of HIV strains for entry into the target cells, is now being targeted for gene therapy to develop HIV resistance in patients. In this review, we discuss different strategies that are being adapted for CCR5-gene disruption in CD4+ T cells and in hematopoietic stem cells (HSCs) to generate a HIV-resistant immune system in infected individuals. If CCR5 gene that can shape HIV-resistant T cells, it will aim in new approaches in clinical trials. But these techniques have certain weaknesses and disadvantages, and will need to be paired with other strategies to form a full HIV remedy. There is also a need to establish methods to help deter HIV re-emergence following targeted CCR5 therapy. But ultimately, this brought us a better knowledge of the road to HIV treatment.
ABSTRACTBackgroundBile Salt Export Pump (BSEP/ABCB11) is important in the maintenance of the ente... more ABSTRACTBackgroundBile Salt Export Pump (BSEP/ABCB11) is important in the maintenance of the enterohepatic circulation of bile acids and drugs. Drugs such as rifampicin, glibenclamide inhibit BSEP. Progressive Familial Intrahepatic Cholestasis Type-2, a lethal pediatric disease, some forms of intrahepatic cholestasis of pregnancy, and drug-induced cholestasis are associated with BSEP dysfunction.MethodsWe started with a bioinformatic approach to identify the relationship between ABCB11 and other proteins, microRNAs, and drugs. Microarray data set of the liver samples from ABCB11 knockout mice was analyzed by GEO2R tool. Differentially expressed gene pathway enrichment analysis was done by ClueGo v2.5.5 app from Cytoscape. Protein-protein interaction network was constructed by STRING application in Cytoscape. Networks were analyzed using the Cytoscape software v3.7.1. CyTargetLinker v4.1.0 was used to screen the transcription factors, microRNAs and drugs. Predicted drugs were validat...
BackgroundGenomic safe harbors are sites in the genome which are safe for gene insertion such tha... more BackgroundGenomic safe harbors are sites in the genome which are safe for gene insertion such that the inserted gene will function properly, and the disruption of the genomic location doesn’t cause any foreseeable risk to the host. The AAVS1 site is the site which is disrupted upon integration of Adeno Associated Virus (AAV) and is considered a ‘safe-harbor’ in human genome because about one third of humans are infected with AAV and so far there is no apodictic evidence that AAV is pathogenic or disruption of AAVS1 causes any disease in man. Therefore, we chose to target AAVS1 site for the insertion of ABCB11, a bile acid transporter which is defective in Progressive Familial Intra Hepatic Cholestasis Type-2 (PFIC-2), a lethal disease of children where cytotoxic bile salts accumulate inside hepatocytes killing them and eventually the patient.MethodsWe used CRISPR Cas9 a genome editing tool to insert ABCB11 gene at AAVS1 site in human cell-lines.ResultsWe found that human ABCB11 sequ...
Background Quantitative sensory testing protocols for perceptions of pleasantness and unpleasantn... more Background Quantitative sensory testing protocols for perceptions of pleasantness and unpleasantness based on the German Research Network on Neuropathic Pain protocol were recently introduced. However, there are no reliability studies yet published. Aim To evaluate the intra-examiner (test-retest) and inter-examiner reliability for orofacial pleasantness and unpleasantness quantitative sensory testing protocols. Methods Sixteen healthy participants from Aarhus University (11 women and five men, mean age 24, range 21–26 years) contributed. Two examiners were trained in performing the entire quantitative sensory testing protocols for pleasantness and unpleasantness, which included the additional dynamic tactile stimulation test using a goat-hair brush. Each participant underwent examination of both protocols by each examiner (inter-examiner reliability) on day 1. They returned at least 8 days following the testing to be re-examined by one examiner (intra-examiner reliability). All tes...
Lactic acid bacteria (LAB)are the essential ingredients in probiotic foods, intestinal microflora... more Lactic acid bacteria (LAB)are the essential ingredients in probiotic foods, intestinal microflora, and dairy products able to cope up and exist in diverse environmental ranges. Samples were collected using sterile test tubes and transported to a laboratory in the icebox for further biochemical characterization. Gram test and catalase activity were examined after microscopically distinct colonies were sub-cultured to pure colonies based on standard gram and catalase test protocols. Subsequently, these bacteria were characterized for their ability to grow at various salt concentrations (5%,10%, and15%) and temperature gradients (15°C, 30°C, 45°C). Acid-tolerance was analyzed by growing the colonies in MRS broth adjusted to acidic pH (pH 3) and pH 7.2 (control). Bile tolerance of LAB isolates was assayed by growing in 0.3% bile-supplemented MRS agar. Bile salt hydrolase (BSH) activity was studied by growing 10 μl of the prepared overnight culture on BSH screening media containing MRS a...
Background/AimBreast cancer is one of the world’s leading cause of deaths in women. This study ev... more Background/AimBreast cancer is one of the world’s leading cause of deaths in women. This study evaluated the in-vitro anticancer activity of different thiosemicarbazones (HacTSc, HSTsc, 3-MBTSc, 4-NBTSc and 4-HBTSc) against MCF-7 human breast cancer cell line and MCF-10 normal cell.Materials and MethodsThe ligands were prepared and characterized by UV vis, IR,1H NMR. MTT assay was used to determine cell viability. Then data were analyzed using two-way ANOVA with Tukey post-hoc test. Result: The ligands have IC50value ranging from 2.271µg/ml to 7.081µg/ml. Acetone thiosemicarbazone and 3-Methoxybenzaldehyde thiosemicarbazone, were identified as the most potent against MCF-7 breast cancer cells with IC50value of 2.271µg/ml and 2.743µg/ml respectively. Whereas 4-Nitrobenzaldehyde thiosemicarbazone was the least potent. Also, the IC50of the normal MCF-10 cell indicated their activity were selective. Conclusion: The activity of the ligands were dose, position and substituents dependent. ...
International journal of biological macromolecules, Jan 15, 2018
Integrin-linked kinase (ILK) is a member of Ser/Thr kinase which interacts to the cytoplasmic dom... more Integrin-linked kinase (ILK) is a member of Ser/Thr kinase which interacts to the cytoplasmic domain of β-integrins, and thereby induces apoptosis. ILK is considered as potential drug target because it's direct involvement in the tumor progression. Here, we have performed molecular docking followed by 100 ns MD simulation to understand the mechanism of interaction of ILK with the ellagic acid (EA). EA is well known for its antiproliferative and antioxidant properties in cancer cell lines and animal models. We have observed that EA binds to the active site cavity of ILK and causes conformational changes in the ILK structure. The orientation of EA in the active pocket of ILK showed to have least RMSD values and stable. The average binding energy ILK-EA complex calculated during MMPBSA was -191.267 kJ/mol, indicating a relatively strong binding affinity. The actual binding affinity of EA to ILK was measured by fluorescence spectroscopy and K and n values were 9.28 μM and 1.9264 (~2...
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Papers by Pankaj Taneja