In this study, we provide a universal approach to Gene Expression Engineering (GeneEE) for creati... more In this study, we provide a universal approach to Gene Expression Engineering (GeneEE) for creating artificial expression systems. GeneEE leads to the generation of artificial 5ʹ regulatory sequences (ARES) consisting of promoters and 5ʹ untranslated regions. The ARES lead to the successful recruitment of RNA polymerase, related sigma factors and ribosomal proteins that result in a wide range of expression levels. We also demonstrate that by engaging native transcription regulators, GeneEE can be used to generate inducible promoters. To showcase the universality of the approach, we demonstrate that 200-nucleotide (nt)-long DNA with random composition can be used to generate functional expression systems in six bacterial species, Escherichia coli, Pseudomonas putida, Corynebacterium glutamicum, Thermus thermophilus, Streptomyces albus and Streptomyces lividans, and the eukaryote yeast Saccharomyces cerevisiae.
Accumulation of organic matter and particles generated from uneaten feed and faeces is one of the... more Accumulation of organic matter and particles generated from uneaten feed and faeces is one of the major challenges in recirculating aquaculture systems (RAS), in particular colloidal and dissolved fractions as these serve as bacterial substrate. Particles can potentially cause damage on the gills of the reared species and may reduce their resistance to pathogens. Most diseases in aquaculture of marine fish are caused by opportunistic bacteria which become pathogenic when the fish is under stressful conditions. In the present study, the consequences of different levels of organic matter removal on physicochemical and microbial water quality were investigated in replicate small-scaled RAS for production of Atlantic salmon parr (Salmo salar). Two RAS were run in parallel over 140 days, including one conventional system (cRAS) and one system with an implemented membrane (mRAS). In mRAS, a side-stream of 10% of the water flow was filtered through an ultrafiltration (UF) membrane prior to...
In this study, we provide a universal approach to Gene Expression Engineering (GeneEE) for creati... more In this study, we provide a universal approach to Gene Expression Engineering (GeneEE) for creating artificial expression systems. GeneEE leads to the generation of artificial 5ʹ regulatory sequences (ARES) consisting of promoters and 5ʹ untranslated regions. The ARES lead to the successful recruitment of RNA polymerase, related sigma factors and ribosomal proteins that result in a wide range of expression levels. We also demonstrate that by engaging native transcription regulators, GeneEE can be used to generate inducible promoters. To showcase the universality of the approach, we demonstrate that 200-nucleotide (nt)-long DNA with random composition can be used to generate functional expression systems in six bacterial species, Escherichia coli, Pseudomonas putida, Corynebacterium glutamicum, Thermus thermophilus, Streptomyces albus and Streptomyces lividans, and the eukaryote yeast Saccharomyces cerevisiae.
Accumulation of organic matter and particles generated from uneaten feed and faeces is one of the... more Accumulation of organic matter and particles generated from uneaten feed and faeces is one of the major challenges in recirculating aquaculture systems (RAS), in particular colloidal and dissolved fractions as these serve as bacterial substrate. Particles can potentially cause damage on the gills of the reared species and may reduce their resistance to pathogens. Most diseases in aquaculture of marine fish are caused by opportunistic bacteria which become pathogenic when the fish is under stressful conditions. In the present study, the consequences of different levels of organic matter removal on physicochemical and microbial water quality were investigated in replicate small-scaled RAS for production of Atlantic salmon parr (Salmo salar). Two RAS were run in parallel over 140 days, including one conventional system (cRAS) and one system with an implemented membrane (mRAS). In mRAS, a side-stream of 10% of the water flow was filtered through an ultrafiltration (UF) membrane prior to...
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Papers by Jenny Nesje