Papers by Hisashi Watanabe
Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 1981
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Horticultural Research (Japan), 2008
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THE JOURNAL OF THE STOMATOLOGICAL SOCIETY,JAPAN, 1993
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Periodontology 2000, 1997
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Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 1984
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SPIE Proceedings, 1996
ABSTRACT
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Dentistry in Japan, 1997
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Journal of Japanese Society for Laser Dentistry, 1993
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THE JOURNAL OF THE STOMATOLOGICAL SOCIETY,JAPAN, 1985
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Lasers in Dentistry VII, 2001
ABSTRACT Application of the erbium: YAG laser to periodontal treatment has been attempted and pre... more ABSTRACT Application of the erbium: YAG laser to periodontal treatment has been attempted and preferable results have been reported for calculus removal, vaporization of granulation tissue, periodontal pocket sterilization and so on. However, it has been difficult to reach and treat some conditions involving complex root morphology and furcated rots with conventional probes. The new broom probe was designed and tested to overcome these obstacles. The probe was made of 20 super-fine optical fibers bound into a broom shape. The experiments were carried out to evaluate the destructive power of a single fiber and to examine the morphology of tissue destruction and the accessibility to a bifurcated root of a human tooth using the broom probe. The Er:YAG laser prototype was used. A flat specimen plate was made by cutting the root of a cow tooth and then attached to an electrically operated table and irradiated under various conditions. The specimens were examined with both an optical and scanning electron microscope. The irradiated surfaces were also examined with a roughness meter. An irradiation applied with a single fiber with an energy level of 1 to 1.5 mJ at its tip results in a destruction depth of 3 to 24 micrometers . The optimum conditions for the fibers of this probe was 1.0 mJ at 10 pps and a scanning speed of 100 mm/min. No part of the tooth surface remained un-irradiated after using the broom probe to cover the surface 5 times parallel to the tooth axis and then five times at a 30 degree angle to the previous irradiation at a power of 20 mJ at 10 pps. Also curved and irregular surface were destroyed to a maximum depth of 19 micrometers . In conclusion, these results suggest that the broom probe would be applicable for periodontal laser treatments even if the tooth surface has a complex and irregular shape.
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Journal of the International Academy of Periodontology, 2003
The use of the laser in dentistry has been widening due to its increasing use in surgery and medi... more The use of the laser in dentistry has been widening due to its increasing use in surgery and medicine. In the field of periodontology, the most commonly used lasers have been CO2 and Nd:YAG lasers. According to the last reviews reporting the use of lasers in periodontal therapy, these lasers were considered unsuitable for dental treatment, due to various shortcomings, such as the carbonization and severe thermal damages caused on the target and surrounding tissues, and therefore their use has been confined to soft tissue procedures. Technological advances and improvements have increased the choices of the available laser systems. Among them, Er:YAG lasers seem to be of promising use. The Er:YAG laser is a solid-state crystal laser operating in the infrared wavelength (2,940 nm). Due to its high absorbability in water and hydroxyapatite, several studies have shown the effectiveness of this laser for both hard and soft tissue ablation, and its bactericidal effects with less or even no...
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Advanced Laser Dentistry, 1995
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Photomedicine and Laser Surgery, 2004
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Periodontology 2000, 2004
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Odontology, 2012
Periodontal regeneration using EDTA or PDGF showed promising results, but the effect of combined ... more Periodontal regeneration using EDTA or PDGF showed promising results, but the effect of combined application was still unclear. This study aimed to verify the effect of EDTA and/or PDGF application on root adhesion and proliferation of PDL fibroblast cells. Eighty specimens were prepared from forty periodontitis teeth and made five groups: (1) diseased (untreated), (2) SRP (scaling root planing), (3) EDTA (24%), (4) PDGF (25 ng/ml) and (5) Combined application of EDTA and PDGF. Periodontal ligament cells were cultured on the above conditioned dentin plate, and SEM examination was preformed and cells were counted within a representative standard area for both cell morphology and density. All groups including untreated showed significantly increase of adhered cells from baseline to 7 days. Among them, rate of increase was much higher in EDTA, PDGF, and combined groups. ANOVA test indicated that the number of cells in PDGF and combined groups was significantly higher than diseased group at 1 day. On day 7, PDGF and combined groups showed significantly higher number of adhesion cells than that found in the diseased, SRP and EDTA groups. Thus, root conditioning with EDTA enhanced cell adhesion more than SRP alone. There was no significant difference of cell number between PDGF and combined group. Combined application of EDTA and PDGF increased significantly PDL cell adhesion than EDTA alone. PDGF alone, however, also showed comparable effect to combined application at all periods. Thus, synergistic effect between PDGF and EDTA was not observed.
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Lasers in Surgery and Medicine, 1996
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Journal of Periodontology, 2007
The use of the erbium-doped:yttrium, aluminum, and garnet (Er:YAG) laser in periodontal therapy h... more The use of the erbium-doped:yttrium, aluminum, and garnet (Er:YAG) laser in periodontal therapy has been the focus of much research. Recombinant human platelet-derived growth factor-BB (rhPDGF-BB) is suggested as a potent stimulator and strong mitogen for human periodontal ligament cells. The present study tested the direct effects of Er:YAG laser irradiation, alone or with rhPDGF-BB application, on the biocompatibility of periodontally diseased roots through fibroblast attachment and proliferation. The study examined five healthy and 15 periodontally involved teeth, prepared from proximal surfaces, which were divided randomly into four groups (10 specimens each): group 1: healthy; group 2: untreated diseased; group 3: Er:YAG laser irradiation (60 mJ/pulse, 10 Hz); and group 4: Er:YAG laser irradiation (60 mJ/pulse, 10 Hz) plus rhPDGF-BB application (50 ng/ml). Three subgroups per group (three specimens each) were incubated for three periods (1, 3, or 7 days). The remaining specimen was used to determine surface topography. Fibroblasts were pooled on root specimens and incubated. Results were evaluated using scanning electron microscopy. Repeated cell counts were performed within a representative standard area. Using paired t tests, all experimental groups (except group 2 diseased) showed statistically significant differences between 1- and 3-day and between 1- and 7-day incubation periods, but not between 3- and 7-day incubation periods. Using analysis of variance, the intergroup comparison showed significant differences favoring group 1 over groups 2 and 3 and group 4 over group 2 at the 1-day incubation period; group 1 was favored over groups 2, 3, and 4 and groups 3 and 4 were favored over group 2 at the 3- and 7-day incubation periods. Comparable effects were shown between groups 3 and 4 for all incubation periods and between groups 2 and 3 and groups 1 and 4 for the 1-day incubation. Er:YAG laser used alone or in combination with rhPDGF-BB application may offer a promising periodontal therapy for conditioning root surfaces, although the combined application seemed to be slightly more effective. However, testing laser use in intervals and with parameters <60 mJ/pulse and 10 Hz is required to verify the minimum threshold values necessary to obtain complete root debridement and clarify optimal conditions for fibroblast cell attachment and growth. Further studies are needed to determine ideal parameters for creating the best environment for successful periodontal treatment.
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Journal of Periodontology, 2005
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Journal of Periodontology, 1993
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Journal of Periodontology, 1999
Hypophosphatasia (HOPS) is an inherited disorder characterized by the defect of skeletal minerali... more Hypophosphatasia (HOPS) is an inherited disorder characterized by the defect of skeletal mineralization due to tissue-nonspecific alkaline phosphatase (TNSALP) deficiency. In this study we analyzed the TNSALP gene from a Japanese patient with HOPS, his parents, his brother, and unrelated normal controls. The proband is a 25-year-old Japanese male diagnosed with childhood hypophosphatasia. The patient reported premature exfoliation of the deciduous teeth and severe periodontal destruction of the permanent dentition. Genomic DNA was extracted from peripheral leukocytes of subjects. Eleven pairs of the polymerase chain reaction (PCR) primers were used to amplify the coding exons according to the published sequence data of the TNSALP gene. The PCR amplified samples were subjected to PCR-single strand conformation polymorphism (SSCP) analysis and PCR-allele specific oligonucleotide (ASO) analysis. In PCR-SSCP analysis of the patient's genomic DNA, the fragments containing exons 9 and 10 revealed abnormal mobilities. These abnormal mobilities (exons 9 and 10) were also found from his mother and father's genomic DNA, respectively. The sequencing analysis of the abnormal bands extracted from the SSCP gel showed a T to C transition at nucleotide position 1155 (T1155C) in exon 9 and G1320A in exon 10. PCR-ASO analysis confirmed these missense point mutations. PCR-ASO analysis also confirmed that mutation-specific oligonucleotides corresponded to the new mutations and did not hybridize with PCR products from normal control genomic DNAs. These results indicated that the proband was a compound heterozygote who inherited T1155C mutation in exon 9 from the mother and G1320A mutation in exon 10 from the father. Both of them are new missense point mutations and appear to cause significant changes in the structure and function of TNSALP.
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Papers by Hisashi Watanabe