Oligonucleotide strands containing dithiol (–SS–) groups were used as the co-crosslinkers in PNIP... more Oligonucleotide strands containing dithiol (–SS–) groups were used as the co-crosslinkers in PNIPA–AAc based nanogels (NGs). They hybridized with PEG–oligonucleotides introduced into the gels. The specific DNA hybrid formed in the nanogel/nanocarrier was involved in highly efficient accumulation of intercalators. The presence of –SS– groups/bridges improved the storing efficiency of doxorubicin (Dox) in DNA hybrids by 53, 40 and 20% compared to regular, single stranded and regular double stranded DNA crosslinkers, respectively. The explicit arrangement of the hybrids in the carrier enabled their reduction by glutathione and an effective cancer treatment while the side toxicity could be reduced. Compared to the NGs with traditional crosslinkers and those containing typical dsDNA-based hybrids, an improved, switchable and controlled drug release occurred in the novel NGs. Since the novel NGs can release the oligonucleotide strands during their degradation, this gives an opportunity fo...
Recently, the fast development of hybrid nanogels dedicated to various applications has been seen... more Recently, the fast development of hybrid nanogels dedicated to various applications has been seen. In this context, nanogels incorporating biomolecules into their nanonetworks are promising innovative carriers that gain great potential in biomedical applications. Hybrid nanogels containing various types of biomolecules are exclusively designed for: improved and controlled release of drugs, targeted delivery, improvement of biocompatibility, and overcoming of immunological response and cell self-defense. This review provides recent advances in this rapidly developing field and concentrates on: (1) the key physical consequences of using hybrid nanogels and introduction of biomolecules; (2) the construction and functionalization of degradable hybrid nanogels; (3) the advantages of hybrid nanogels in controlled and targeted delivery; and (4) the analysis of the specificity of drug release mechanisms in hybrid nanogels. The limitations and future directions of hybrid nanogels in targeted...
Switchable conformational changes of multiresponsive nanogels containing disulfide/DNA hybrid she... more Switchable conformational changes of multiresponsive nanogels containing disulfide/DNA hybrid shells for pulsative drug release.
Three-segment oligonucleotide hybrids introduced as crosslinkers to a PNIPA–AAc nanonetwork can b... more Three-segment oligonucleotide hybrids introduced as crosslinkers to a PNIPA–AAc nanonetwork can be specifically transformed and degraded. Architecture of presented carrier helped to achieve enhanced drug loading and tunable and degradable gel properties, and to control release of the drug.
Biocompatible nanohydrogels modified with three-segment oligonucleotide hybrids were used for con... more Biocompatible nanohydrogels modified with three-segment oligonucleotide hybrids were used for controlled loading and prolonged release of anticancer intercalators in hyperthermia treatment.
Abstract The aquation of the core of two novel cisPt complexes led to the formation of diaquated ... more Abstract The aquation of the core of two novel cisPt complexes led to the formation of diaquated and monoaquated forms for two examined Pt complexes with peptides. DNA extent of DNA platination by novel cisPt derivatives was estimated. This extent was dependent on base-sequence in DNA and type of cisPt complex. The electroreduction of the novel Pt complexes and their adducts with DNA at a GC electrode led to the formation of Pt nanoparticles. In consequence catalytic hydrogen evolution (HER) currents appeared; they were characterized in function of DNA platination extent and size, distribution and mass of electrodeposited Pt NPs. Linear relations were obtained for catalytic HER current and mass of electrodeposited Pt NPs vs. extent of DNA platination for novel peptide cisPt compounds. They have electroanalytical significance.
Composites consisting of ss- and ds-DNA strands and polyacrylamide (PAM) hydrogel have been synth... more Composites consisting of ss- and ds-DNA strands and polyacrylamide (PAM) hydrogel have been synthesized. DNA was entrapped non-covalently. The obtained DNA biomaterial exhibited a strong increase in guanine and adenine anodic currents when temperature reached the physiological level. This increase was related to the unique oligonucleotide structural changes in the composite. The structural alterations in the PAM lattices were employed for the release of the drug accumulated in the composite. Doxorubicin (Dox) was selected as the drug; it was accumulated by intercalation to dsDNA and was slowly released from the dsDNA/PAM system by using a minor temperature increase (up to 40÷45°C) as it is routinely done in hyperthermia. The applied release temperature was either constant or oscillating. The binding strength, the rate of Dox release and the properties of the composite were examined using voltammetry, SEM and ICP-MS.
The electrooxidation of double-stranded DNA (dsDNA) from calf thymus was carried by using cyclic ... more The electrooxidation of double-stranded DNA (dsDNA) from calf thymus was carried by using cyclic voltammetry. A glassy carbon disk-, a platinum disk-, a platinum mesh- and a carbon vapor-deposited platinum mesh electrodes were used. It is shown that the appropriate chemical and biological (steam treatment) purification of the complete cell allows, for the graphite electrode, formation of a wide anodic dsDNA signal with two visible anodic peaks. There was no necessity of preaccumulation of dsDNA on the electrode surface and of use of mediators to get well defined voltammetric signals. These peaks apparently reflect electrooxidation of the DNA's guanine and adenine. The spectrophotometric data obtained during the electrooxidation indicate that the absorbance increases with an increase in potential and electrooxidation current of dsDNA. However, the absorption band maximum either does or does not change its position depending on the mesh material. This different spectroscopic behav...
Oligonucleotide strands containing dithiol (–SS–) groups were used as the co-crosslinkers in PNIP... more Oligonucleotide strands containing dithiol (–SS–) groups were used as the co-crosslinkers in PNIPA–AAc based nanogels (NGs). They hybridized with PEG–oligonucleotides introduced into the gels. The specific DNA hybrid formed in the nanogel/nanocarrier was involved in highly efficient accumulation of intercalators. The presence of –SS– groups/bridges improved the storing efficiency of doxorubicin (Dox) in DNA hybrids by 53, 40 and 20% compared to regular, single stranded and regular double stranded DNA crosslinkers, respectively. The explicit arrangement of the hybrids in the carrier enabled their reduction by glutathione and an effective cancer treatment while the side toxicity could be reduced. Compared to the NGs with traditional crosslinkers and those containing typical dsDNA-based hybrids, an improved, switchable and controlled drug release occurred in the novel NGs. Since the novel NGs can release the oligonucleotide strands during their degradation, this gives an opportunity fo...
Recently, the fast development of hybrid nanogels dedicated to various applications has been seen... more Recently, the fast development of hybrid nanogels dedicated to various applications has been seen. In this context, nanogels incorporating biomolecules into their nanonetworks are promising innovative carriers that gain great potential in biomedical applications. Hybrid nanogels containing various types of biomolecules are exclusively designed for: improved and controlled release of drugs, targeted delivery, improvement of biocompatibility, and overcoming of immunological response and cell self-defense. This review provides recent advances in this rapidly developing field and concentrates on: (1) the key physical consequences of using hybrid nanogels and introduction of biomolecules; (2) the construction and functionalization of degradable hybrid nanogels; (3) the advantages of hybrid nanogels in controlled and targeted delivery; and (4) the analysis of the specificity of drug release mechanisms in hybrid nanogels. The limitations and future directions of hybrid nanogels in targeted...
Switchable conformational changes of multiresponsive nanogels containing disulfide/DNA hybrid she... more Switchable conformational changes of multiresponsive nanogels containing disulfide/DNA hybrid shells for pulsative drug release.
Three-segment oligonucleotide hybrids introduced as crosslinkers to a PNIPA–AAc nanonetwork can b... more Three-segment oligonucleotide hybrids introduced as crosslinkers to a PNIPA–AAc nanonetwork can be specifically transformed and degraded. Architecture of presented carrier helped to achieve enhanced drug loading and tunable and degradable gel properties, and to control release of the drug.
Biocompatible nanohydrogels modified with three-segment oligonucleotide hybrids were used for con... more Biocompatible nanohydrogels modified with three-segment oligonucleotide hybrids were used for controlled loading and prolonged release of anticancer intercalators in hyperthermia treatment.
Abstract The aquation of the core of two novel cisPt complexes led to the formation of diaquated ... more Abstract The aquation of the core of two novel cisPt complexes led to the formation of diaquated and monoaquated forms for two examined Pt complexes with peptides. DNA extent of DNA platination by novel cisPt derivatives was estimated. This extent was dependent on base-sequence in DNA and type of cisPt complex. The electroreduction of the novel Pt complexes and their adducts with DNA at a GC electrode led to the formation of Pt nanoparticles. In consequence catalytic hydrogen evolution (HER) currents appeared; they were characterized in function of DNA platination extent and size, distribution and mass of electrodeposited Pt NPs. Linear relations were obtained for catalytic HER current and mass of electrodeposited Pt NPs vs. extent of DNA platination for novel peptide cisPt compounds. They have electroanalytical significance.
Composites consisting of ss- and ds-DNA strands and polyacrylamide (PAM) hydrogel have been synth... more Composites consisting of ss- and ds-DNA strands and polyacrylamide (PAM) hydrogel have been synthesized. DNA was entrapped non-covalently. The obtained DNA biomaterial exhibited a strong increase in guanine and adenine anodic currents when temperature reached the physiological level. This increase was related to the unique oligonucleotide structural changes in the composite. The structural alterations in the PAM lattices were employed for the release of the drug accumulated in the composite. Doxorubicin (Dox) was selected as the drug; it was accumulated by intercalation to dsDNA and was slowly released from the dsDNA/PAM system by using a minor temperature increase (up to 40÷45°C) as it is routinely done in hyperthermia. The applied release temperature was either constant or oscillating. The binding strength, the rate of Dox release and the properties of the composite were examined using voltammetry, SEM and ICP-MS.
The electrooxidation of double-stranded DNA (dsDNA) from calf thymus was carried by using cyclic ... more The electrooxidation of double-stranded DNA (dsDNA) from calf thymus was carried by using cyclic voltammetry. A glassy carbon disk-, a platinum disk-, a platinum mesh- and a carbon vapor-deposited platinum mesh electrodes were used. It is shown that the appropriate chemical and biological (steam treatment) purification of the complete cell allows, for the graphite electrode, formation of a wide anodic dsDNA signal with two visible anodic peaks. There was no necessity of preaccumulation of dsDNA on the electrode surface and of use of mediators to get well defined voltammetric signals. These peaks apparently reflect electrooxidation of the DNA's guanine and adenine. The spectrophotometric data obtained during the electrooxidation indicate that the absorbance increases with an increase in potential and electrooxidation current of dsDNA. However, the absorption band maximum either does or does not change its position depending on the mesh material. This different spectroscopic behav...
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