Papers by Adriana Ada Ceverista Chiappetta
Bookmarks Related papers MentionsView impact
Plant Physiology, Feb 1, 1999
Bookmarks Related papers MentionsView impact
Plant Physiology, Aug 1, 2001
Bookmarks Related papers MentionsView impact
Plants
Gene duplication played a fundamental role in eukaryote evolution and different copies of a given... more Gene duplication played a fundamental role in eukaryote evolution and different copies of a given gene can be present in extant species, often with expressions and functions differentiated during evolution. We assume that, when such differentiation occurs in a gene copy, this may be indicated by its maintenance in all the derived species. To verify this hypothesis, we compared the histological expression domains of the three β-glucuronidase genes (AtGUS) present in Arabidopsis thaliana with the GUS evolutionary tree in angiosperms. We found that AtGUS gene expression overlaps in the shoot apex, the floral bud and the root hairs. In the root apex, AtGUS3 expression differs completely from AtGUS1 and AtGUS2, whose transcripts are present in the root cap meristem and columella, in the staminal cell niche, in the epidermis and in the proximal cortex. Conversely, AtGUS3 transcripts are limited to the old border-like cells of calyptra and those found along the protodermal cell line. The G...
Bookmarks Related papers MentionsView impact
International Journal of Molecular Sciences
Anthocyanins protect plants against various biotic and abiotic stresses, and anthocyanin-rich foo... more Anthocyanins protect plants against various biotic and abiotic stresses, and anthocyanin-rich foods exert benefits on human health due to their antioxidant activity. Nevertheless, little information is available on the influence of genetic and environmental factors on the anthocyanin content in olive fruits. Based on this consideration, the total anthocyanin content, the genes involved in anthocyanin biosynthesis, and three putative R2R3-MYB transcription factors were evaluated at different ripening stages in the drupes of the Carolea and Tondina cultivars, sampled at different altitudes in the Calabria region, Italy. During drupe ripening, the total anthocyanin content and the transcript levels of analyzed genes gradually increased. In line with the anthocyanin content, a different level of expression of anthocyanin structural genes was observed in ‘Carolea’ compared to ‘Tondina’, and in relation to the cultivation area. Furthermore, we identified Oeu050989.1 as a putative R2R3-MYB...
Bookmarks Related papers MentionsView impact
Agrochimica
Bookmarks Related papers MentionsView impact
Acta Horticulturae
Bookmarks Related papers MentionsView impact
Frontiers in Plant Science
IntroductionPeach (Prunus persica (L.) Batsch,) and nectarine fruits (Prunus persica (L.) Batsch,... more IntroductionPeach (Prunus persica (L.) Batsch,) and nectarine fruits (Prunus persica (L.) Batsch, var nectarine), are characterized by a rapid deterioration at room temperature. Therefore, cold storage is widely used to delay fruit post-harvest ripening and extend fruit commercial life. Physiological disorders, collectively known as chilling injury, can develop typically after 3 weeks of low-temperature storage and affect fruit quality.MethodsA comparative transcriptomic analysis was performed to identify regulatory pathways that develop before chilling injury symptoms are detectable using next generation sequencing on the fruits of two contrasting cultivars, one peach (Sagittaria) and one nectarine, (Big Top), over 14 days of postharvest cold storage.ResultsThere was a progressive increase in the number of differentially expressed genes between time points (DEGs) in both cultivars. More (1264) time point DEGs were identified in ‘Big Top’ compared to ‘Sagittaria’ (746 DEGs). Both cu...
Bookmarks Related papers MentionsView impact
Foods
Cold storage is used to extend peach commercial life, but can affect quality. Quality changes are... more Cold storage is used to extend peach commercial life, but can affect quality. Quality changes are assessed through the content of nutritionally relevant compounds, aroma, physical characters and/or sensorially. Here, six peach and nectarine cultivars were sampled at commercial harvest and after 7 days of 1 °C storage. A trained panel was used to evaluate sensorial characters, while carotenoids, phenolics, vitamin C, total sugars, and qualitative traits including firmness, titrable acidity and soluble solid content were integrated with volatile organic compound (VOC) analysis previously reported. The different analyses reveal interesting patterns of correlation, and the six cultivars responded differently to cold storage. Sensory parameters were correlated with 64 VOCs and seven intrinsic characters. Acidity, firmness, and 10 VOCs were strongly negatively correlated with harmony and sweetness, but positively correlated with bitterness, astringency, and crunchiness. In contrast, Brix,...
Bookmarks Related papers MentionsView impact
First Symposium on Horticulture in Europe, 2008
Bookmarks Related papers MentionsView impact
102° Congresso della Società Botanica Italiana, 2007
Bookmarks Related papers MentionsView impact
Cost 822 development of integrated systems for large scale propagation of elite plants using in vitr, 2000
Bookmarks Related papers MentionsView impact
Plant Science, 2022
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Figure S4. Interactive pathways analysis during drupe maturation of 'Carolea' population ... more Figure S4. Interactive pathways analysis during drupe maturation of 'Carolea' population growing at 200 masl. The red and blue lines indicate the up and down regulated pathways respectively. (PPTX 935 kb)
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Le \u3b2-glucuronidasi (GUS) sono enzimi che catalizzano l\u2019idrolisi del legame glicosidico f... more Le \u3b2-glucuronidasi (GUS) sono enzimi che catalizzano l\u2019idrolisi del legame glicosidico fra l\u2019acido \u3b2-glucuronico e altre molecole e sono suddivise in due famiglie: glicosil-idrolasi 2 e glicosil-idrolasi 79. Le \u3b2-glucuronidasi della famiglia 2, alle quali appartiene il GUS di E. coli largamente usato come gene reporter nei vegetali, sono note in un grande range di organismi, ma sembrano essere assenti nelle piante, nelle quali l\u2019attivit\ue0 GUS endogena sembra associata a \u3b2-glucuronidasi della famiglia 79 (Eparanasi) (1). In Arabidpopsis thaliana sono stati identificati e parzialmente caratterizzati tre diversi geni GUS (AtGUS1, AtGUS2 e AtGUS3), dei quali AtGUS1 e AtGUS2 mostrano elevata omologia. A parte funzioni specifiche (2),coinvolgimento delle \u3b2-glucuronidasi \ue8 stato proposto nel processo di accrescimento cellulare per distensione e, in particolare, nell\u2019allungamento dei peli radicali (3). E\u2019 stato, inoltre, ipotizzato che \u3b2-glucuronidasi, agendo in coordinazione con glucuronisiltrasferasi (UGT) del gruppo 85A, siano responsabili della regolazione del ciclo cellulare (4,5). Uno studio pi\uf9 recente tuttavia ha messo in parte in discussione quanto emerso in precedenza riportando che nelle radici di plantule di Arabidopsis thaliana non \ue8 presente attivit\ue0 \u3b2-glucuronidasica (6). In questo lavoro si \ue8 voluto approfondire lo studio dell\u2019espressione dei geni GUS e UGT in Arabidopsis. Verifiche preliminari effettuate con PCR semiquantitativa hanno mostrato che, nonostante il gene AtGUS2 risulti espresso in modo pi\uf9 rilevante rispetto a AtGUS1 e AtGUS3, tutti e tre i geni AtGUS sono espressi, sia pure differenzialmente, in tutti gli organi della pianta. I geni AtUGT85A mostranoun pattern di espressione pi\uf9 differenziato rispetto ai geni AtGUS. AtUGT85A1, AtUGT85A2 e AtUGT85A4 risultano differenzialmente espressi nei vari organi della pianta, mentre AtUGT85A3 e UGT85A7 mostrano un\u2019espressione organo specifica che coinvolge fiore e foglia Utilizzando la tecnica di ibridazione in situ con sonde multiple recentemente messa a punto su campioni vegetali (7), l\u2019espressione dei geni GUS \ue8 stata studiata anche a livello tissutale. A livello radicale , il pattern di espressione dei tre geni analizzati appare chiaramente differenziato: AtGUS1 e AtGUS2 sono espressi nell\u2019epidermide, nella zona corticale e nella cuffia, mentre l\u2019espressione di AtGUS3 \ue8 limitata all\u2019epidermide. Sono in corso le analisi relative agli altri organi della pianta e, in particolare, fiore e polline. Analogo approccio verr\ue0 esteso ai geni AtUGT85 al fine di verificarne l\u2019overlapping o meno con i domini istologici di espressione dei geni GUS e definirne eventuali correlazioni
Bookmarks Related papers MentionsView impact
Dottorato di ricerca in Biologia Vegetale,Ciclo XXVI,a.a.2013
Bookmarks Related papers MentionsView impact
Uploads
Papers by Adriana Ada Ceverista Chiappetta