The extent of joining is maximal at 4 degrees and decreases with increasing temperatures following sigmoid-like curves.
Jan 10, 1981 · The temperature dependence of the T4 DNA ligase-catalyzed joining of plasmid DNA linearized by the action of HaeIII, EcoRI and PstI ...
The temperature dependence of the T4 DNA ligase-catalyzed joining of plasmid DNA linearized by the action of HaeIII, EcoRI and PstI restriction endonucleases ...
Temperature dependence of the joining by T4 DNA ligase of termini produced by type II restriction endonucleases. Luca Ferretti and Vittorio Sgaramella.
PDF | The temperature dependence of the T4 DNA ligase-catalyzed joining of plasmid DNA linearized by the action of HaeIII, EcoRI and PstI restriction.
The temperature dependence of the T4 DNA ligase-catalyzed joining of plasmid DNA linearized by the action of HaeIII, EcoRI and PstI restriction endonucleases ...
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TEMPERATURE DEPENDENCE OF THE JOINING BY T4 DNA LIGASE OF TERMINI PRODUCED BY TYPE II RESTRICTION ENDONUCLEASES. Author: FERRETTI L; SGARAMELLA V
Temperature dependence of the joining by T4 DNA ligase of termini produced by type I I restriction endonucleases. Nucleic Acids Res. 1981; 9(1):85-93. pmid ...
Salt-T4 DNA Ligase will join cohesive end termini at salt concentrations as high as 300 mM without any loss in activity. Salt-T4 DNA Ligase is insensitive to ...
The temperature of optimum ligase activity is 37°C. The cohesive ends generated by restriction endonuclease digestion are short. For the joining of fragments ...