We found that the cell viabilities were influenced by both of cooling and warming rates in CPA-free cryopreservation.

Influence of cooling and warming rates to cell viability in cryoprotectant-free cryopreservation. Hiroki Watanabe, Hideyo Takizawa, Yoshitake Akiyama.

Mar 4, 2019 · One study showed that warming rates in the range 100 °C min−1 to 2 °C min−1 had very little effect on the viability of mouse lymphocytes ...

The simulation result showed that the cooling rate in our method was much faster than in the previous studies. Moreover, we represented that the cell viability ...

Apr 1, 2019 · We demonstrate a method of CPA-free cryopreservation, in which cells are almost vitrified by ultrarapid cooling using inkjet printing.

Nov 16, 2022 · The cooling rate influences both processes [16]. The boundaries of a cell's viability are determined by an intact plasma membrane with its ...

The optimal freezing rate depends on two independent factors: cell size and water permeability [15]. In hematopoietic stem cells, a cooling rate of −1 °C / min ...

Jul 18, 2022 · Slow cooling rates (<1 °C min−1) allow cells sufficient time to dehydrate and prevents IIF. However, cells become exposed to high solute ...

Influence of cooling and warming rates to cell viability in cryoprotectant-free cryopreservation · Improved and reproducible cell viability in the superflash ...

Both cooling modes led to comparable results in terms of the motility, fertilization ability, and DNA integrity of the warmed spermatozoa. In both cases, ...