This issue probably have been discussed, my anti-virus (Mcaffe-Livesafe) quarantined the file:
C:\Program Files\Python37\Lib\site-packages\PyInstaller\bootloader\Windows-32bit\run.exe

My first thought is false positive, but by checking the file in Virus total it is marked in 10 different anti-virus engines:

My questions:
1- is this file really a malware?

2- if it is safe, is having it missing affect the application?