Europe PMC
Nothing Special   »   [go: up one dir, main page]

Europe PMC requires Javascript to function effectively.

Either your web browser doesn't support Javascript or it is currently turned off. In the latter case, please turn on Javascript support in your web browser and reload this page.

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

The nucleotide sequence of the promoter and the amino-terminal region of alkaline phosphatase structural gene (phoA) of Escherichia coli.

Nucleic Acids Research, 01 Nov 1981, 9(21):5671-5678
https://doi.org/10.1093/nar/9.21.5671 PMID: 6273802 PMCID: PMC327551

Free full text in Europe PMC

Abstract 

The promoter and the amino-terminal region of phoA, the structural gene for alkaline phosphatase of Escherichia coli K12, was cloned by using a promoter cloning vector pMC1403. The nucleotide sequence of the cloned fragment has been determined. A sequence encoding the amino-terminal portion of mature alkaline phosphatase is found and it is preceded by a sequence encoding the signal peptide. The signal peptide consists of 21 amino acids; Met-Lys-Gln-Ser-Thr-Ile-Ala-Leu-Ala-Leu-Leu-Pro-Leu-Leu-Phe-Thr-Pro-Val-Thr-Lys-Ala. The translation initiation codon is GUG, which is preceded by the Shine-Dalgarno sequence GGAG. Upstream to these sequences, there is a typical procaryotic promoter. TATAGTC for the Pribnow box. Around the Pribnow box, there are several dyad symmetrical sequences, which may probably be concerned with the regulation of this gene.

Free full text 

Logo of narLink to Publisher's site
Nucleic Acids Res. 1981 Nov 11; 9(21): 5671–5678.
PMCID: PMC327551
PMID: 6273802

The nucleotide sequence of the promoter and the amino-terminal region of alkaline phosphatase structural gene (phoA) of Escherichia coli.

Y Kikuchi, K Yoda, M Yamasaki, and G Tamura
Copyright and License information Disclaimer

Abstract

The promoter and the amino-terminal region of phoA, the structural gene for alkaline phosphatase of Escherichia coli K12, was cloned by using a promoter cloning vector pMC1403. The nucleotide sequence of the cloned fragment has been determined. A sequence encoding the amino-terminal portion of mature alkaline phosphatase is found and it is preceded by a sequence encoding the signal peptide. The signal peptide consists of 21 amino acids; Met-Lys-Gln-Ser-Thr-Ile-Ala-Leu-Ala-Leu-Leu-Pro-Leu-Leu-Phe-Thr-Pro-Val-Thr-Lys-Ala. The translation initiation codon is GUG, which is preceded by the Shine-Dalgarno sequence GGAG. Upstream to these sequences, there is a typical procaryotic promoter. TATAGTC for the Pribnow box. Around the Pribnow box, there are several dyad symmetrical sequences, which may probably be concerned with the regulation of this gene.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (653K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
 
icon of scanned page 5671
5671
icon of scanned page 5672
5672
icon of scanned page 5673
5673
icon of scanned page 5674
5674
icon of scanned page 5675
5675
icon of scanned page 5676
5676
icon of scanned page 5677
5677
icon of scanned page 5678
5678
 

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • TORRIANI A. Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli. Biochim Biophys Acta. 1960 Mar 11;38:460–469. [Abstract] [Google Scholar]
  • MALAMY M, HORECKER BL. The localization of alkaline phosphatase in E. coli K12. Biochem Biophys Res Commun. 1961 Jun 2;5:104–108. [Abstract] [Google Scholar]
  • Bachmann BJ, Low KB. Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980 Mar;44(1):1–56. [Europe PMC free article] [Abstract] [Google Scholar]
  • Yagil E, Bracha M, Lifshitz Y. The regulatory nature of the phoB gene for alkaline phosphatase synthesis in Escherichia coli. Mol Gen Genet. 1975;137(1):11–16. [Abstract] [Google Scholar]
  • Wanner BL, Latterell P. Mutants affected in alkaline phosphatase, expression: evidence for multiple positive regulators of the phosphate regulon in Escherichia coli. Genetics. 1980 Oct;96(2):353–366. [Europe PMC free article] [Abstract] [Google Scholar]
  • ECHOLS H, GAREN A, GAREN S, TORRIANI A. Genetic control of repression of alkaline phosphatase in E. coli. J Mol Biol. 1961 Aug;3:425–438. [Abstract] [Google Scholar]
  • Willsky GR, Bennett RL, Malamy MH. Inorganic phosphate transport in Escherichia coli: involvement of two genes which play a role in alkaline phosphatase regulation. J Bacteriol. 1973 Feb;113(2):529–539. [Europe PMC free article] [Abstract] [Google Scholar]
  • Wanner BL, Sarthy A, Beckwith J. Escherichia coli pleiotropic mutant that reduces amounts of several periplasmic and outer membrane proteins. J Bacteriol. 1979 Oct;140(1):229–239. [Europe PMC free article] [Abstract] [Google Scholar]
  • Inouye H, Beckwith J. Synthesis and processing of an Escherichia coli alkaline phosphatase precursor in vitro. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1440–1444. [Europe PMC free article] [Abstract] [Google Scholar]
  • Blobel G, Dobberstein B. Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma. J Cell Biol. 1975 Dec;67(3):835–851. [Europe PMC free article] [Abstract] [Google Scholar]
  • von Heijne G, Blomberg C. Trans-membrane translocation of proteins. The direct transfer model. Eur J Biochem. 1979 Jun;97(1):175–181. [Abstract] [Google Scholar]
  • Emr SD, Hall MN, Silhavy TJ. A mechanism of protein localization: the signal hypothesis and bacteria. J Cell Biol. 1980 Sep;86(3):701–711. [Europe PMC free article] [Abstract] [Google Scholar]
  • Sarthy A, Fowler A, Zabin I, Beckwith J. Use of gene fusions to determine a partial signal sequence of alkaline phosphatase. J Bacteriol. 1979 Sep;139(3):932–939. [Europe PMC free article] [Abstract] [Google Scholar]
  • Casadaban MJ, Chou J, Cohen SN. In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals. J Bacteriol. 1980 Aug;143(2):971–980. [Europe PMC free article] [Abstract] [Google Scholar]
  • Torriani A. Alkaline phosphatase subunits and their dimerization in vivo. J Bacteriol. 1968 Oct;96(4):1200–1207. [Europe PMC free article] [Abstract] [Google Scholar]
  • Cohen SN, Chang AC, Hsu L. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110–2114. [Europe PMC free article] [Abstract] [Google Scholar]
  • Klein RD, Selsing E, Wells RD. A rapid microscale technique for isolation of recombinant plasmid DNA suitable for restriction enzyme analysis. Plasmid. 1980 Jan;3(1):88–91. [Abstract] [Google Scholar]
  • Colman A, Byers MJ, Primrose SB, Lyons A. Rapid purification of plasmid DNAs by hydroxyapatite chromatography. Eur J Biochem. 1978 Nov 2;91(1):303–310. [Abstract] [Google Scholar]
  • Sutcliffe JG. pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long. Nucleic Acids Res. 1978 Aug;5(8):2721–2728. [Europe PMC free article] [Abstract] [Google Scholar]
  • Bradshaw RA, Cancedda F, Ericsson LH, Neumann PA, Piccoli SP, Schlesinger MJ, Shriefer K, Walsh KA. Amino acid sequence of Escherichia coli alkaline phosphatase. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3473–3477. [Europe PMC free article] [Abstract] [Google Scholar]
  • Kelley PM, Neumann PA, Shriefer K, Cancedda F, Schlesinger MJ, Bradshaw RA. Amino acid sequence of Escherichia coli alkaline phosphatase. Amino- and carboxyl-terminal sequences and variations between two isozymes. Biochemistry. 1973 Aug 28;12(18):3499–3503. [Abstract] [Google Scholar]
  • Nakata A, Yamaguchi M, Izutani K, Amemura M. Escherichia coli mutants deficient in the production of alkaline phosphatase isozymes. J Bacteriol. 1978 Apr;134(1):287–294. [Europe PMC free article] [Abstract] [Google Scholar]
  • Lazdunski C, Baty D, Pagès JM. Procaine, a local anesthetic interacting with the cell membrane, inhibits the processing of precursor forms of periplasmic proteins in Escherichia coli. Eur J Biochem. 1979 May 2;96(1):49–57. [Abstract] [Google Scholar]
  • Steege DA. 5'-Terminal nucleotide sequence of Escherichia coli lactose repressor mRNA: features of translational initiation and reinitiation sites. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4163–4167. [Europe PMC free article] [Abstract] [Google Scholar]
  • Shine J, Dalgarno L. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342–1346. [Europe PMC free article] [Abstract] [Google Scholar]
  • Pribnow D. Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter. Proc Natl Acad Sci U S A. 1975 Mar;72(3):784–788. [Europe PMC free article] [Abstract] [Google Scholar]
  • Dickson RC, Abelson J, Barnes WM, Reznikoff WS. Genetic regulation: the Lac control region. Science. 1975 Jan 10;187(4171):27–35. [Abstract] [Google Scholar]
Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

Full text links 

Read article at publisher's site: https://doi.org/10.1093/nar/9.21.5671

Read article for free, from open access legal sources, via Unpaywall: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC327551Unpaywall

Citations & impact 

Impact metrics

63
Citations
Jump to Citations
7
Data citations
Jump to Data

Citations of article over time

Alternative metrics

Altmetric item for https://www.altmetric.com/details/41124899
Altmetric
Discover the attention surrounding your research
https://www.altmetric.com/details/41124899

Article citations

Go to all (63) article citations

Data 

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.