Table 4

Comparison of rhythms in PER2::LUC in +/+, Afh/+ and Afh/Afh mice from slice cultures recorded in PMTs

	N	% slices rhythmic	Period (h)	Amplitude (cpm)
SCN
+/+	24	100	24.04 ± 0.25	15076 ± 2512
Afh/+	10	100	24.61 ± 0.37	7847 ± 3275
Afh/Afh	21	95.2	25.61 ± 0.27	7123 ± 1484
PIT
+/+	24	100	23.59 ± 0.17	20924 ± 3000
Afh/+	13	84.6	24.68 ± 0.9	5995 ± 1992
Afh/Afh	15	93.3	26.63 ± 0.44*	2311 ± 417*
Arc complex
+/+	25	88	23.3 ± 0.35	830 ± 230
Afh/+	14	71.4	24.3 ± 0.56	796 ± 246
Afh/Afh	21	52.4	24.9 ± 0.49	168 ± 37
DMH
+/+	25	84	23.13 ± 0.56	587 ± 127
Afh/+	14	78.6	22.88 ± 0.74	629 ± 165
Afh/Afh	21	52.4	26.16 ± 0.91*	229.9 ± 40
Hb
+/+	12	66.7	22.11 ± 0.10	514 ± 143
Afh/+	5	80	23.91 ± 0.98	417 ± 117
Afh/Afh	14	50	24.68 ± 1.11	205 ± 103

Values are expressed as mean ± SEM. Bold underlined values are statistically different from +/+ mice. *Indicates statistically significant differences between Afh/+ and Afh/Afh groups. Arc, arcuate nucleus; DMH, dorsomedial hypothalamus; Hb, habenula; PIT, pituitary; SCN, suprachiasmatic nucleus.