Detection and separation of the submetacentric marker chromosome of the WALKER (W-256) carcinoma using flow cytometry and sorting.

Abstract

The chromosomes of WALKER (W-256) carcinoma cells have been separated into different DNA subclasses using DAPI for quantitative DNA staining and laser flow cytometry. The submetacentric marker chromosome could be isolated and its DNA content was determined to be 1.3 pg. One microgram marker DNA was obtained after separation of about 750 000 marker chromosomes by means of electronic flow sorting. The chromosomal composition of sorted fractions was analyzed by microscopy following banding of sorted chromosomes. The average morphological purity obtained was about 83%.

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Articles referenced by this article (16)

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Abstract

Full text links

References

Homogeneously staining regions and double minutes in a human cell line: chromatin organization and DNA content.

Interaction of the RNA polymerase inhibitor chromomycin with DNA.

Assignment of snRNA gene sequences to the large chromosomes of rat kangaroo and chinese hamster isolated by flow cytometric sorting.

Phenotypic and genotypic characteristics of a cell line from a squamous cell carcinoma of human skin.

Carrano AV, van Dilla MA, Gray JW (1979) Flow-cytogenetics: A new approach to chromosome analysis. In: Melamed MR, Mullaney PF, Mendelsohn ML (eds) Flow cytometry and sorting. John Wiley, New York, pp 421–451

Separation of large quantities of chromosomes by velocity sedimentation at unit gravity.

Chromosome measurement and sorting by flow systems.

Cytochemical studies of metaphase chromosomes by flow cytometry.

Interactions of heteroaromatic compounds with nucleic acids. A - T-specific non-intercalating DNA ligands.

Sandberg AA (1981) (ed) The chromosomes in human cancer and leukemia, 1st edn, Elsevier, Amsterdam, pp 427–457

Citations & impact

Impact metrics

Citations of article over time

Article citations

Specific and rapid enumeration of viable but nonculturable and viable-culturable gram-negative bacteria by using flow cytometry.

Application of laser optical tweezers in immunology and molecular genetics.

On-line sorting of human chromosomes by centromeric index, and identification of sorted populations by GTG-banding and fluorescent in situ hybridization.

Detection and chromosomal assignment of SV40-DNA integration in Chinese hamster cell lines by chromosome sorting and dot blot hybridization.

Karyotypic change from heteroploidy to near diploidy associated with development of cisplatin resistance in a rat ovarian tumour cell line.

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