Abstract

Introduction

Protein aggregation diseases are human disorders associated with, and hallmarked by, the formation of protein aggregates in afflicted patients [1]. These aggregates often share a common structural classification: an amyloid or amyloid-like fibrillar structure, representing long filaments with a characteristic ‘cross-β’ type architecture. For many protein aggregation diseases we now have a detailed understanding of the structures formed by aggregated proteins. The current article will review recent developments in the structural biology of polyglutamine (polyQ) protein aggregation, with a focus on the role played by magic angle spinning (MAS) solid-state nuclear magnetic resonance (ssNMR) spectroscopy.

polyQ diseases

The polyQ protein diseases are CAG repeat expansion disorders: inherited neurodegenerative diseases connected to the genetic expansion of CAG codon repeats in various human genes [2]. This family includes several different ataxias and Huntington's disease (HD). In each disorder, a particular gene that harbors a CAG repeat element is mutated. Individuals in which the CAG repeat is expanded beyond a certain threshold (often in the range of 35–50 CAG repeats) are at risk for disease. Notably, the age of onset is inversely correlated to the repeat length, with juvenile cases stemming from long expansion lengths. Naturally, the mutated genes yield mutant proteins featuring an expanded polyQ segment somewhere in the mutant protein (Figure 1A–C). These mutant proteins, or fragments thereof, form inclusions or aggregates [3]. The aggregation propensity is correlated to the polyQ length [4]. Most knowledge of the molecular mechanisms, the mutant proteins, and the aggregates is available for the most common polyQ disorder: HD [5]. This review will focus on HD, with readers referred to other literature for more information on other polyQ disorders [2,6,7].

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Figure 1.

Summary of HttEx1 polyQ protein structures.

(A,B) Domain architecture of protein (fragments) from the most common polyQ diseases: HttEx1 from Huntington's disease (HD) and ataxin-3 from spinocerebellar ataxia 3 (SCA3). The polyQ segment is shown in yellow, alongside neighboring flanking domains with and without folded domains. HttEx1 is disordered in its monomeric state. (C) Sequence of HttEx1, with the location of prominent post-translational modifications indicated. The three key parts of HttEx1 are marked: N17, polyQ, and PRD. (D,E) X-ray fiber diffraction and negative stain transmission EM of aggregated polyQ/HttEx1. Adapted with permission from Hoop et al. [28]. (F) Structural model of aggregated HttEx1 from ssNMR and other techniques. (G) The polyQ core showing the block-like architecture that buries most of the polyQ segments. (F,G) Adapted with permission from Boatz et al. [33], under its CC-BY license.

In HD, the mutated protein is huntingtin (Htt), with the CAG repeat in its first exon (HttEx1; Figure 1A,C). Full-length Htt exceeds 3000 amino acids, and its structure in the non-aggregated state has been solved by cryo-EM [8,9]. However, in patients and model animals, the aggregating species is not the full-length protein, but rather N-terminal fragments containing the expanded polyQ [10]. Much research focuses on fragments that coincide with HttEx1, as it appears to play an important pathogenic role [11,12], even though other types of N-terminal fragments may be relevant as well. Until now the precise mechanism by which the HD mutation (and mutant protein) can lead to disease, remains uncertain. That said, many studies of HD pathogenesis focus on HttEx1 as a representative model for the suspected proteinaceous disease-causing agent, invoking a gain-of-toxic-function mechanism associated with HttEx1 misfolding and aggregation.

Earlier structural studies of polyQ protein aggregates

Since the 1990s, there has been much interest in the structure of aggregated polyQ proteins. Electron microscopy (EM; Figure 1E) and atomic force microscopy (AFM) showed the aggregates to be elongated filaments [3,13]. A noted feature of HttEx1 fibrils is their heterogeneous (or fuzzy) appearance, and propensity for higher-order clustering. X-ray fibril diffraction showed that aggregated polyQ proteins display the characteristic cross-β signature common for amyloid-forming polypeptides (Figure 1D) [14–16]. The cross-β pattern of polyQ aggregates suggests a 4.8 Å strand-to-strand distance in the hydrogen bonding direction, and a 8.2 Å sheet-to-sheet distance between the sheets [14]. Thus, polyQ protein fibrils display the hallmarks of amyloid or amyloid-like fibrils.

Cryo-EM

Recent years have seen dramatic improvements in cryo-EM, including in its application to amyloid fibrils [17]. In the HD field, the structure of the un-aggregated full-length Htt was determined by single-particle averaging [8,9]. In it, HttEx1 and its polyQ segment are not visible due to inherent disorder and flexibility. Aggregates formed by the HttEx1 fragment have been subjected to both cryo-EM and electron tomography (ET), in cells and in vitro [18–20]. These studies provided a view of the supramolecular architecture of HttEx1 fibrils and their polymorphism, and permitted a comparison between cellular aggregates and those prepared from pure proteins. The cryo-ET studies provide a compelling perspective on the way the HttEx1 fibrils interact with each other and with subcellular compartments and membranes. They also report a high degree of similarity between HttEx1 fibrils aggregated in a cellular context and in vitro. Individual fibrils have a width of multiple nm, but these fibrils cluster into larger assemblies, which become visible as puncta in fluorescence microscopy studies. Similar conclusions were drawn by recent super-resolution studies, which visualized nm-sized filaments alongside the larger clusters typically detected by (confocal) microscopy [21]. Unlike dramatic atomic-resolution structures obtained for other amyloid fibrils [17], no such high-resolution EM structures are available for any polyQ protein fibrils. A recent paper [20] provided the first results from cryo-EM analysis of HttEx1 fibrils, yielding improved insights into their architecture and their polymorphism (more below; Figure 3D). However, the obtained data did not permit an atomic-level structure to be determined, due to fibril heterogeneity and disorder. Another challenge for cryo-EM analysis is that the fibrils lack a clear and systematic twist, which is helpful for cryo-EM image analysis and atomic structure reconstruction.

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Figure 3.

Studies of the HttEx1 flanking domains and their interactions.

(A) 2D ssNMR spectrum obtained by scalar-recoupling-based ssNMR spectroscopy, showing the flexible flanking domains of HttEx1 fibrils. (B) Comparison of 1D ¹³C traditional (top; CP) and scalar (bottom; INEPT) ssNMR spectra for different HttEx1 polymorphs, revealing primarily differences in flanking domain motion in the bottom spectra. (C) Negative-stain TEM of the same Q44-HttEx1 fibril types, which differed in their fibril widths. (D) Cryo-EM data on a sub-class of Q23-HttEx1 fibrils, showing an architecture mediated by flanking domain interactions. The two images represent different views on the same fibril type. (E) Model of flanking domain entanglement in protofilament interactions in wider HttEx1 fibrils (e.g. type 2 shown in B,C), deduced from combined analysis of negative stain TEM and ssNMR data. (A–C,E) Adapted with permission from Lin et al. [32], under the CC license. Panel (D) Reprinted with permission from Nazarov et al. [20] Copyright 2022 American Chemical Society.

ssNMR of polyQ amyloid cores

Since more than 15 years, ssNMR has been used to determine the structures of various pathogenic and functional amyloids [22–24]. Those structure determinations incorporated also constraints from EM (and other) techniques to generate full atomic-resolution structures. At the current time, no (peer-reviewed) atomic structure for polyQ or HttEx1 fibrils has yet been published (although a preprinted report on their integrative structure determination was posted in 2023 [25], but I here focus on the peer-reviewed literature). Historically, the literature on ssNMR of polyQ aggregates began in 2011, with two reports from different groups [26,27]. Combined, these early studies identified several key features of polyQ amyloid structure. Independent of the polyQ length, or the presence or absence of Htt-derived flanking sequences, the glutamine residues in the polyQ core gave a characteristic doubled set of ssNMR signals (illustrated in Figure 2A,B, from [28]). As discussed in a later study [29], these signals are atypical for normal glutamine conformations, and seem to represent a ssNMR signature for polyQ amyloid. Strikingly, two sets of signals make up this signature, being present in equal intensities. When targeted residue-specific isotope labeling is applied, this peak doubling is not removed, independent of the labeled site (Figure 2A, top) [26,28,30]. Thus, it is apparent that each of two sets of peaks represents the summed signals of many individual glutamine residues at different sequence positions in the protein. This contrasts with non-repetitive (amyloid) proteins, in which one aims to observe one peak per sequence position (i.e. per residue). Yet, the ssNMR (¹³C) peak linewidth of fully labeled polyQ proteins does not differ dramatically from aggregates in which isolated residues were labeled (Figure 2A top vs bottom; and refs. [26,28–31]). This means that there is a high degree of structural order and homogeneity in the fibril core, despite the morphological heterogeneity and disorder seen by cryo-EM (above). The recent literature contains a series of papers from different ssNMR groups that continue to show the remarkable consistency of this polyQ ssNMR signature in many polyQ and HttEx1 fibril polymorphs (Figures 2A and ​and3B)3B) [28,30,32–37]. This is notably different from ssNMR studies of e.g. Aβ or α-synuclein polymorphs, where ssNMR signals of the amyloid core residues vary significantly between preparations and between research groups [38–40]. Thus, ssNMR suggests that polyQ-driven aggregation typically yields a reproducible, highly ordered, and characteristic amyloid core architecture [28,41].

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Figure 2.

Example ssNMR data on the structure of polyQ amyloid cores.

(A) Comparison of 2D ¹³C-¹⁵N ssNMR spectra (NCA and NCO spectra) for three different polyQ aggregates, showing identical chemical shifts for the polyQ core. The ssNMR signature of the polyQ core features two sets of signals, coined forms a and b (marked in red and blue, respectively), in equal intensities. Note that the bottom samples are fully labeled HttEx1 protein fibrils, whilst the top shows a sample in which only two glutamines are selectively ¹³C,¹⁵N-labeled. (B) Part of a 2D ¹³C-¹³C CP-DARR ssNMR spectrum showing the same two glutamine conformers, in equal intensities. A smaller third peak (form c) is attributed to residues outside the buried core. (C) Schematic showing the molecular origin of the red/blue conformers (forms a/b) in the antiparallel β-sheets of polyQ amyloid. (D) Published schematic showing the glutamine side chain interdigitation in the core, based on ssNMR structural analysis. (E) PolyQ torsion angle ssNMR restraints for the backbone dihedral angles, shown in a Ramachandran plot. Horizontal red/blue areas mark permitted regions based on dipolar recoupling measurements of the Ψ angle (NCCN experiments), while the diamonds mark chemical-shift-based estimates from the TALOS program. (F) ssNMR data curves that constrain the side chain torsion angles χ₁ and χ₂ of polyQ amyloid, based on HCCH-type dihedral angle measurements. The red and blue datapoints represent the two Gln conformers, along with lines showing the best-fit dihedral angles. (A,B–F) Adapted with permission from Hoop et al. [28].

The determination of protein structures by ssNMR typically depends on measurements of distances between specific residues, using dipolar recoupling techniques [42]. However, the glutamine residues in the polyQ core all behave the same — a particular glutamine has a 50:50 chance to adopt one of the two hallmark signals. Even residue-specific labeling (achievable by solid-phase peptide synthesis [26,30] or using Amber-codon approaches [43]) yields the same pattern (Figure 2A). Therefore, distance measurements as a means toward an atomic-resolution structure are at best highly challenging. Does this mean that ssNMR structure determination is not feasible in polyQ amyloid? Previous structural studies of amyloidogenic polypeptides [44–46] deployed measurements of another structural parameter: torsion angles [47]. Specialized ssNMR experiments allow the determination of the relative orientations of neighboring chemical bonds (e.g. N–H, C–H or C–N bonds), based on the recoupling of dipolar interactions or chemical shift anisotropy tensors. This allows one to constrain the backbone (Ramachandran angles) and side-chain torsion angles (rotamer states). For a more in-depth discussion of these experiments, readers are referred to a recent review article [47]. In polyQ amyloids, these experiments were applied to backbone and side-chain dihedral angles of glutamine residues within the amyloid core (Figure 2E,F) [25,28]. These torsion angle measurements showed unambiguously that the two signals of the polyQ signature stem from two different glutamine conformations. Both conformations reflect β-sheet structure, but they differ in their Ramachandran angles (Figure 2E) and rotamer states (Figure 2F). These findings were based on the application of so-called NCCN and HCCH dihedral angle measurements, where dipolar interactions were recoupled between carbons, nitrogens and protons [28,47]. Antiparallel β-sheets are inherently expected (and observed) to assemble by alternating β-strands with different backbone conformations (Figure 2C; red/blue arrows) [48]. This alternating pattern offers an elegant explanation of the observation that two signals are typically observed in equal intensities in polyQ amyloids, independent of the sample type (Figure 2A). Moreover, 2D ssNMR and IR studies using mixtures of isotope-labeled proteins provided evidence that polyQ-based aggregates (with long polyQ segments) contain β-hairpin structures (Figure 2C) [28,49]. Such experiments exemplify the way that targeted isotope labeling can be deployed to distinguish intra- from intermolecular interactions. The β-hairpin motifs could be recognized by detecting the presence of backbone-backbone interactions in samples where the ¹³C-labeled protein was diluted with non-¹³C proteins. The detected interactions could only be explained by intramolecular hydrogen bonding as found in β-hairpin structures.

ssNMR side-chain dihedral angle measurements identified differences in the χ₁ torsion angles of the two dominant glutamine conformers (Figure 2F) [28]. These were experiments where the relative orientations of the C–H bonds in the side chain were measured, using dipolar recoupling experiments. These measurements also showed that the χ₂ angle was close to 180° for both conformers. Similar angular constraints were also reported by Raman spectroscopy [50]. Combining these structural insights, one can build a picture of the internal structure of the polyQ amyloid core, with glutamine side chains extended into an interdigitated ‘steric zipper’ interface (Figure 2D) [25,28]. The steric zipper concept describes a hallmark feature of amyloids, which is that they often feature a dehydrated, highly stable core structure in which amino acid sidechains interdigitate into a tight zipper-like configuration [51]. A related concept is the idea of polyQ polar zippers, as originally proposed by Perutz et al. [16]: ‘linking β-strands together into sheets or barrels by networks of hydrogen bonds between their main-chain amides and between their polar side chains’. However, back in 1994, these authors lacked the atomic-resolution data to build an experiment-based atomic-level structure for polyQ. Notably, other glutamine-rich amyloids feature such steric or polar zippers [45,51–55], but it is important to stress that those fibrils typically feature parallel β-sheets, whilst the antiparallel structure (Figure 2C) of the polyQ fibril core makes it qualitatively different. The use of ssNMR now enables structural measurements that can elevate general amyloid concepts to an atomic-level understanding of core of polyQ amyloids, shared by polyQ model polypeptides and disease-relevant HttEx1 fibrils.

A strength and limitation of ssNMR is its sensitivity to sub-nm structural features. On the one hand, this means that ssNMR can provide atomic-level details even in absence of high-order structural homogeneity (unlike X-ray diffraction). However, it also means that (fibril) structures determined by ssNMR rely on the integration of sub-nm information from ssNMR with longer-range information from techniques such as EM, ET, or AFM. Thus, ssNMR fibril structures are nice examples of ‘integrative structural biology’ [56]. EM and AFM studies showed the filaments of polyQ-expanded HttEx1 to be several nm in width, both in vitro and in cells. Combining this with inter-sheet and inter-strand distances known from X-ray fiber diffraction (above) and structural ssNMR details, one can start to build a representative architecture (Figures 1F,G and ​and3)3) [25,32,33]. Zooming out beyond the ‘zippered’ side chains, and antiparallel β-sheets, the ssNMR data are only explainable by a type of block-like architecture, with multiple β-sheets stacked side by side (Figure 1G) [28,32,33]. Notably, this type of architecture was previously suggested for polyQ aggregates, based on lower-resolution fiber diffraction and mutational studies [14,57]. More recently, cryo-EM analysis provided more corroboration of this key aspect of the HttEx1 fibril architecture (Figure 3D) [20]. As noted above, this EM analysis did not achieve atomic resolution due to fibril heterogeneity, but it did support key features deduced previously from ssNMR-based analyses. Thus, combining the insights from different techniques (with a key role for ssNMR), we have obtained a good understanding of the internal architecture of polyQ amyloid.

Going beyond the (polyQ) core: HttEx1 flanking domains

The polypeptide chain beyond the polyQ segment cannot be ignored. In HD, the HttEx1 flanking segments have dramatic effects on both aggregation propensity and kinetics [26,60,61], and they determine also the fate and effects of HttEx1 aggregates in cells [62–64]. The N-terminal segment (known as N17 or Htt^NT) boosts aggregation, whilst the C-terminal proline-rich domain (PRD) delays it (Figure 1A,C). The flanking domains (in particular N17) contain residues that are impacted by post-translational modification (PTM): phosphorylation, acetylation, and ubiquitination (Figure 1C). The interest in these PTMs is substantial, as they prevent aggregation, reduce toxicity (in animal models) and facilitate degradation processes [65–67]. Also chaperone proteins that are able to disaggregate pre-formed amyloid, seem to target non-core flanking segments as essential binding sites [63,68]. MAS ssNMR has been used to elucidate the structural fate of these flanking segments in a variety of HttEx1 fibril types [26,32–35,37,69,70]. These studies showed the flanking domains to lack the β-sheet structure and rigidity of the amyloid core. Other ssNMR experiments probed the solvent-exposure of the flanking segments, showing them to be much more solvent-exposed than the polyQ amyloid core.

A key early finding was that the aggregation-enhancing N17 segment was partly α-helical in HttEx1-like peptide fibrils [26,31]. Later studies of various HttEx1 fibril types showed that different fibril polymorphs differ in their N17 structure and dynamics [32,33,35,37]. The flexibility or mobility was even more striking for the C-terminal PRD. In ssNMR and electron spin resonance studies parts of the PRD were remarkably flexible, behaving similar to an intrinsically disordered protein (Figure 3A,B) [69,71]. Notably, proximal to the polyQ core, the PRD becomes more and more ordered, leading to a gradient of flexibility along the C-terminal end of HttEx1, as can be detected via NMR relaxation measurements [69]. Such ssNMR studies were previously also applied to other fibrils featuring a ‘fuzzy coat’ of flexible polypeptide segments outside the fibril core [38,72]. This includes MAS ssNMR experiments that are based on similar principles as common in liquid-state NMR: the use of through-bond or scalar-interactions to transfer magnetization (signal) between nuclei (INEPT experiments). In their normal implementation, rigid parts of the sample (e.g. the fibril core) are invisible in such experiments, due to the short ¹H T₂ relaxation times (in absence of ¹H decoupling). This suppresses all signals, except those from highly flexible protein segments (Figure 3A,B) [58,73]. Such MAS ssNMR experiments probed the secondary structure, dynamics and interactions of the flexible flanking domains of HttEx1 fibrils [32,35,69]. This revealed the rigid polyQ core to be decorated with flexible, exposed, flanking domains (Figure 1F,G). This molecular architecture manifests in EM as a fuzzy coat, or a bottle-brush type architecture [71], a feature also seen in other pathogenic amyloid fibrils [74,75].

ssNMR views of supramolecular HttEx1 polymorphism

One important aspect of the fuzzy coat of HttEx1 fibrils is that it plays a striking role in HttEx1 fibril polymorphism [32,37]. Amyloid polymorphism describes the observation that most amyloid-forming polypeptides form an array of different kinds of fibrils depending on conditions. The interest in these ‘polymorphs’ stems from the fact that their different structures translate into differences in biological properties such as fibril stability and toxicity [40]. Moreover, once formed, certain fibril types are able to propagate themselves by recruiting soluble protein monomers and catalyzing their transformation into the amyloid state. The existence of fibril polymorphs can offer a rationale for conflicting findings regarding the cellular toxicity of protein aggregates. Even if certain types of fibril assemblies are measured to have low cytotoxicity [76], other polymorphs may be much more pathogenic, complicating direct comparisons unless one validates the polymorph structure between measurements.

The sensitivity of ssNMR to atomic-level details has made it a valuable tool for detecting and dissecting amyloid polymorphs. Commonly, amyloid polymorphs have distinct ssNMR spectra with peaks arising at different chemical shift values [38–40]. A striking feature of HttEx1 fibrils is that different preparations do not differ in peak positions, even when they can look dramatically different by EM, have different polyQ lengths, or are prepared by different research groups [26–29,32,33,35,37]. What does this mean? When HttEx1 is aggregated under different conditions, EM measurements and antibody binding assays can show differences in the fibrils [32,37,77]. Yet, ssNMR spectra on these samples yield very similar spectra in which peak positions do not change, arguing that the local atomic-level structure is not different. Then how can these be distinct polymorphs? Clues are found in dynamics seen by ssNMR: different polymorphs differ in the dynamics and solvent-accessibility of flanking domains. This pointed to a model in which the HttEx1 aggregates tend to contain very similarly structured fibril cores (on the atomic level; Figure 2A) but supramolecular interactions within and between protofilaments differ (Figure 3D,E). Here, the term protofilament refers to smaller filaments that wrap around each other to form wider fibrils. The ssNMR data make sense if HttEx1 fibrils are assembled from thinner protofilaments (~4–6 nm wide), with varying interactions between the protofilaments mediated by flanking domains (and in particular the PRD). This idea is reminiscent of the subsequently introduced concept of ‘supramolecular’ or ‘ultrastructural’ polymorphism, in which tau fibrils were found to contain similarly structured protofilaments engaged in a variety of supramolecular interactions [78]. Crucially, this model has found direct support from recent cryo-EM analysis of HttEx1 fibril structure (Figure 3D) [20]. Aside from differences in the flanking segments (and their interactions), the polyQ literature (and ssNMR studies therein) also suggest changes or variations in the supramolecular architecture of the polyQ segment itself. As discussed above, ssNMR on expanded polyQ protein aggregates revealed β-hairpins within the fibril core [28]. Shorter polyQ peptides or proteins appear to lack the β-hairpin, even if they still form the same type of antiparallel β-sheet architecture [41]. Thus, it has been suggested that there is a polyQ-dependent switch in aggregation mechanisms, which may explain the polyQ threshold phenomenon in the respective diseases [29,41,79]. Notably, as yet, ssNMR has been one of the few techniques that was able to structurally analyze these types of hairpin structures in polyQ fibrils [28,29,49,80,81].

Challenges and future directions

Most ssNMR studies discussed above were performed using proteins or peptides with isotope enrichment (¹³C and/or ¹⁵N). This enables multidimensional spectroscopy and speeds up NMR analysis. Until now, this has limited the ssNMR to aggregates prepared from purified proteins, as the production of isotope-enriched materials in neuronal cells or model animals remains challenging. An important future goal is to test directly whether aggregates formed in cells, animals, or patients are different in structure. One way is to pursue ssNMR analysis of unlabeled proteins. The literature includes many studies in which the signature peaks of polyQ amyloids were detected in absence of isotope labeling [26,81,90,91]. One particularly promising novel approach is the combination of ssNMR with dynamic nuclear polarization (DNP), which boosts the ssNMR sensitivity by orders of magnitude, making 2D DNP-ssNMR experiments on unlabeled proteins practical [90,91]. An alternative approach has been deployed in other amyloid studies [92]: using unlabeled ex vivo aggregates as seeds for the aggregation of isotope-labeled proteins. Such experiments could test whether the seeded aggregates feature polyQ core signatures, or if this may open up different aggregation pathways [93]. Fortunately, the study of polyQ proteins will also benefit from ongoing advances in ssNMR technology. These developments include the increasing availability of higher-field ssNMR magnets, ever-faster MAS spinning rates, ¹H-detected MAS NMR, and cryogenically cooled MAS NMR probes. The application of these novel technologies will enable the study of (much) smaller sample sizes and the detection of more subtle structural/dynamic effects. Especially the former can be valuable to pursue ex vivo samples, in-cell studies and difficult-to-prepare samples (e.g. based on segmental- or residue-specific labeling techniques[43,94]).

An obvious (and necessary) future direction is the ssNMR analysis of other polyQ disease proteins. Until now, ssNMR data are limited to polyQ model peptides and derivatives of the HttEx1 protein. MAS ssNMR analysis of polyQ-expanded ataxin-1 or ataxin-3 aggregates would be very interesting, even just to see if such aggregates contain a common polyQ core structure. Naturally, the flanking segments would differ completely from those in HttEx1. It is likely that those proteins also yield heterogeneous aggregates, with disordered regions, leaving again an important role for ssNMR analysis. Even in the context of HD, there are many open questions related to the impact of PTMs, different polyQ lengths, Htt fragments, interactions with membranes, chaperones and other proteins.

In conclusion, this mini-review set out to provide a basic overview of key contributions of ssNMR to our understanding of the polyQ protein aggregates. This research area illustrates how ssNMR can play an essential role in the study of amyloid fibrils, illuminating aspects not easily determined by other structural techniques. The ability to probe the fibrils’ dynamic fuzzy coat (flanking domains) was discussed in some detail, and how this is connected to important biological questions relevant to disease. Many open questions remain to be answered, with a continued role for ssNMR as a key technique in the integrative structural biology of polyQ disease proteins, as well as other protein aggregates and biomolecular complexes.

Perspectives

• Protein aggregation into amyloid-like fibrils is a hallmark feature of the CAG repeat expansion, or polyglutamine expansion, disorders — a whole family of incurable inherited neurodegenerative diseases. The structural fate of the mutant proteins in their misfolded state is an essential part of the pathogenic mechanism.

• ssNMR spectroscopy has been an essential technique for understanding the structure of aggregated polyQ proteins, and in particular the mutant huntingtin exon 1 from HD. Surprising structural features of the polymorphic huntingtin aggregates shed light on their biological properties.

• Future applications of ssNMR promise to widen our understanding of other polyglutamine disease proteins, further deepen our knowledge of the molecular disease mechanisms and pave the way for better diagnostics and treatments.

Abbreviations

References