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Quantitative metabolomics of a xylose-utilizing Saccharomyces cerevisiae strain expressing the Bacteroides thetaiotaomicron xylose isomerase on glucose and xylose.

Author information

Affiliations

  • 1. Unit for Environmental Sciences and Management: Microbiology, North-West University, Private Bag X6001, Potchefstroom, 2520, South Africa.
      Authors
    • Mert MJ 1
    • la Grange DC 1
    (2 authors)
  • 2. Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa.
      Authors
    • Rose SH 2
    • van Zyl WH 2
    (2 authors)
  • 3. Department of Chemical Science and Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, 657-8501, Japan.
      Authors
    • Bamba T 3
    • Kondo A 3
    (2 authors)
  • 4. Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, 657-8501, Japan.
      Authors
    • Hasunuma T 4
    (1 author)

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Journal of Industrial Microbiology & Biotechnology, 25 Jul 2017, 44(10):1459-1470
https://doi.org/10.1007/s10295-017-1969-1 PMID: 28744577 

Abstract 

The yeast Saccharomyces cerevisiae cannot utilize xylose, but the introduction of a xylose isomerase that functions well in yeast will help overcome the limitations of the fungal oxido-reductive pathway. In this study, a diploid S. cerevisiae S288c[2n YMX12] strain was constructed expressing the Bacteroides thetaiotaomicron xylA (XI) and the Scheffersomyces stipitis xyl3 (XK) and the changes in the metabolite pools monitored over time. Cultivation on xylose generally resulted in gradual changes in metabolite pool size over time, whereas more dramatic fluctuations were observed with cultivation on glucose due to the diauxic growth pattern. The low G6P and F1,6P levels observed with cultivation on xylose resulted in the incomplete activation of the Crabtree effect, whereas the high PEP levels is indicative of carbon starvation. The high UDP-D-glucose levels with cultivation on xylose indicated that the carbon was channeled toward biomass production. The adenylate and guanylate energy charges were tightly regulated by the cultures, while the catabolic and anabolic reduction charges fluctuated between metabolic states. This study helped elucidate the metabolite distribution that takes place under Crabtree-positive and Crabtree-negative conditions when cultivating S. cerevisiae on glucose and xylose, respectively.

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Read article at publisher's site: https://doi.org/10.1007/s10295-017-1969-1

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Funding 

Funders who supported this work.

Japan Society for the Promotion of Science

    National Research Foundation (1)