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A single method for cryofixation and correlative light, electron microscopy and tomography of zebrafish embryos.

Author information

Affiliations

  • 1. Institute for Molecular Bioscience, University of Queensland, Queensland 4072, Brisbane, Australia.
      Authors
    • Nixon SJ 1
    (1 author)

ORCIDs linked to this article

Traffic (Copenhagen, Denmark), 20 Nov 2008, 10(2):131-136
https://doi.org/10.1111/j.1600-0854.2008.00859.x PMID: 19054388 

Abstract 

The zebrafish is a powerful vertebrate system for cell and developmental studies. In this study, we have optimized methods for fast freezing and processing of zebrafish embryos for electron microscopy (EM). We show that in the absence of primary chemical fixation, excellent ultrastructure, preservation of green fluorescent protein (GFP) fluorescence, immunogold labelling and electron tomography can be obtained using a single technique involving high-pressure freezing and embedding in Lowicryl resins at low temperature. As well as being an important new tool for zebrafish research, the maintenance of GFP fluorescence after fast freezing, freeze substitution and resin embedding will be of general use for correlative light and EM of biological samples.

Full text links 

Read article at publisher's site: https://doi.org/10.1111/j.1600-0854.2008.00859.x

Read article for free, from open access legal sources, via Unpaywall: https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1600-0854.2008.00859.xUnpaywall

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