Karyopherin

Karyopherins are proteins involved in transporting molecules between the cytoplasm and the nucleus of a eukaryotic cell. The inside of the nucleus is called the karyoplasm (or nucleoplasm). Generally, karyopherin-mediated transport occurs through nuclear pores which act as a gateway into and out of the nucleus. Most proteins require karyopherins to traverse the nuclear pore.

Karyopherins can act as importins (i.e. helping proteins get into the nucleus) or exportins (i.e. helping proteins get out of the nucleus). They belong to the nuclear pore complex family^[1] in the transporter classification database (TCDB). Energy for transport is derived from the Ran gradient.

Upon stress, several karyopherins stop shuttling between the nucleus and the cytoplasm and are sequestered in stress granules, cytoplasmic aggregates of ribonucleoprotein complexes.^[2]^[3]

Importin beta

Importin beta is a variety of karyopherin that facilitates the transport of cargo proteins into the nucleus. First, it is binding importin alpha – another type of karyopherin that binds the cargo protein in the cytoplasm—before the cargo protein is imported into the nucleus through the nuclear pore using energy derived from the Ran gradient. Once inside the nucleus, the cargo dissociates from the karyopherins.

Importin beta can also carry proteins into the nucleus without the aid of the importin alpha adapter protein.^[4]

Human genes in the karyopherin family

Additional images

The Ran-GTP cycle

References

^ "Tcdb » Search".
^ Mahboubi, Hicham; Seganathy, Evangeline; Kong, Dekun; Stochaj, Ursula (2013-06-27). "Identification of Novel Stress Granule Components That Are Involved in Nuclear Transport". PLOS ONE. 8 (6): e68356. Bibcode:2013PLoSO...868356M. doi:10.1371/journal.pone.0068356. PMC 3694919. PMID 23826389.
^ Fujimura, Ken; Suzuki, Tomonori; Yasuda, Yoshinari; Murata, Masayuki; Katahira, Jun; Yoneda, Yoshihiro (2010-07-01). "Identification of importin α1 as a novel constituent of RNA stress granules". Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1803 (7): 865–871. doi:10.1016/j.bbamcr.2010.03.020. PMID 20362631.
^ Poon, I. K. H. and D. A. Jans (2005). "Regulation of nuclear transport: Central role in development and transformation?" Traffic 6(3): 173-186.

External links

Karyopherins at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
Illustrations at berkeley.edu
Karyopherin animations
Karyopherin illustrations
3D electron microscopy structures of exportin from the EM Data Bank(EMDB)

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[1] "Tcdb » Search".

[2] Mahboubi, Hicham; Seganathy, Evangeline; Kong, Dekun; Stochaj, Ursula (2013-06-27). "Identification of Novel Stress Granule Components That Are Involved in Nuclear Transport". PLOS ONE. 8 (6): e68356. Bibcode:2013PLoSO...868356M. doi:10.1371/journal.pone.0068356. PMC 3694919. PMID 23826389.

[3] Fujimura, Ken; Suzuki, Tomonori; Yasuda, Yoshinari; Murata, Masayuki; Katahira, Jun; Yoneda, Yoshihiro (2010-07-01). "Identification of importin α1 as a novel constituent of RNA stress granules". Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1803 (7): 865–871. doi:10.1016/j.bbamcr.2010.03.020. PMID 20362631.

[4] Poon, I. K. H. and D. A. Jans (2005). "Regulation of nuclear transport: Central role in development and transformation?" Traffic 6(3): 173-186.

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