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Article

Antioxidant Capacities and Total Phenolic Contents of 56 Wild Fruits from South China

by
Li Fu
1,5,
Bo-Tao Xu
2,
Xiang-Rong Xu
3,
Xin-Sheng Qin
4,
Ren-You Gan
1 and
Hua-Bin Li
1,*
1
Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China
2
Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
3
Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
4
College of Forestry, South China Agricultural University, Guangzhou 510640, China
5
Liwan District Center for Disease Control and Prevention, Guangzhou 510176, China
*
Author to whom correspondence should be addressed.
Molecules 2010, 15(12), 8602-8617; https://doi.org/10.3390/molecules15128602
Submission received: 8 October 2010 / Revised: 17 November 2010 / Accepted: 24 November 2010 / Published: 29 November 2010
(This article belongs to the Special Issue Phenolics and Polyphenolics)
Browse Figures
Figure 1
<p>Correlation between total antioxidant capacities measured by the FRAP and TEAC assays.</p> "> Figure 2
<p>Correlation between the antioxidant capacities and total phenolic content. Antioxidant capacities were measured by the FRAP assay (A) and TEAC assay (B), respectively. GAE: gallic acid equivalents.</p> ">
Versions Notes

Abstract

:
In order to identify wild fruits possessing high nutraceutical potential, the antioxidant activities of 56 wild fruits from South China were systematically evaluated. The fat-soluble components were extracted with tetrahydrofuran, and the water-soluble ones were extracted with a 50:3.7:46.3 (v/v) methanol-acetic acid-water mixture. The antioxidant capacities of the extracts were evaluated using the ferric reducing antioxidant power (FRAP) and Trolox equivalent antioxidant capacity (TEAC) assays, and their total phenolic contents were measured by the Folin–Ciocalteu method. Most of these wild fruits were analyzed for the first time for their antioxidant activities. Generally, these fruits had high antioxidant capacities and total phenolic contents. A significant correlation between the FRAP value and the TEAC value suggested that antioxidant components in these wild fruits were capable of reducing oxidants and scavenging free radicals. A high correlation between antioxidant capacity and total phenolic content indicated that phenolic compounds could be the main contributors to the measured antioxidant activity. The results showed that fruits of Eucalyptus robusta, Eurya nitida, Melastoma sanguineum, Melaleuca leucadendron, Lagerstroemia indica, Caryota mitis, Lagerstroemia speciosa and Gordonia axillaris possessed the highest antioxidant capacities and total phenolic contents among those tested, and could be potential rich sources of natural antioxidants and functional foods. The results obtained are very helpful for the full utilization of these wild fruits.

1. Introduction

It has been proven that free radicals play an important role in many diseases, such as cardiovascular diseases, cancer, neurodegenerative diseases, diabetes and ageing [1,2,3]. Antioxidants have attracted more and more attention as potential agents for preventing and treating oxidative stress-related diseases. The antioxidants comprise both synthetic and natural antioxidants. The synthetic antioxidants have been widely used in the food industry to prolong the shelf life, but some synthetic antioxidants, such as butylated hydroxytoluene and butylated hydroxyanisole, have been found to be harmful due to their potential toxicity and carcinogenicity [4]. On the other hand, epidemiological studies have found that the intake of fruits and vegetables has a strong inverse correlation with the risk of developing many chronic diseases, such as cardiovascular diseases and cancer [5,6,7,8,9]. Vitamins and polyphenols in fruits and vegetables are considered to be responsible for such antioxidant activity, with polyphenols being the most active [10,11]. The natural antioxidants are expected to be an alternative to synthetic ones because of their potential health benefits [12,13]. Antioxidant activities of many fruits, vegetables, spices, medicinal plants and microalgae have been evaluated, and the results showed that some of them could be rich sources of natural antioxidants [14,15,16,17,18,19,20,21,22,23,24,25], so the search for raw materials containing potent natural antioxidants continues to attract the attention of researchers.
Wild fruits are receiving increasing interest from researchers because of their medicinal properties, nutritional value, vitamin and mineral contents. Concerning their medicinal properties, the most commonly studied benefit is their antioxidant effects [26,27,28,29]. There is an abundance of wild fruits in China because of its vast territory, various landforms and wide-spanning climate. Some of these wild fruits are edible, while some can be used as medicines. However, many wild fruits in China are underutilized, and no report about antioxidant activities of wild fruits from China could be found in the literature.
The aim of this study was to systematically evaluate the antioxidant activities and total phenolic contents of 56 wild fruits from South China, to screen out wild fruits with high antioxidant capacity and total phenolic content, and to investigate the relationship of antioxidant capacity and total phenolic content. The results from this study should prove helpful for the full utilization of these wild fruits.

2. Results and Discussion

2.1. Antioxidant capacities of the 56 wild fruits

Fifty-six wild fruit samples were collected from different regions of South China, and were authenticated by a botanist. The scientific names of the plants and sampling location of the wild fruit are shown in Table 1.
Table
Table 1. The scientific names, sampling location and FRAP values (μmol Fe(II)/g) of 56 wild fruits.
Table 1. The scientific names, sampling location and FRAP values (μmol Fe(II)/g) of 56 wild fruits.
No.Scientific Name of PlantSampling LocationFRAP values (μmol Fe(II)/g)
Water-soluble fractionFat-soluble fractionTotal
1Mallotus apeltaGuangdong6.15 ± 0.3616.2 ± 1.6322.4 ± 1.99
2Smilax chinaGuangdong35.0 ± 2.6984.8 ± 5.86120 ± 8.55
3Gardenia jasminoidesGuangdong8.25 ± 0.114.42 ± 0.1312.7 ± 0.24
4Helicteres angustifoliaGuangdong12.2 ± 0.242.15 ± 0.1414.4 ± 0.38
5Eucalyptus robustaGuangdong125 ± 2.49377 ± 3.92502 ± 6.41
6Ficus benjaminaGuangdong6.34 ± 0.381.92 ± 0.138.26 ± 0.51
7Ilex rotundaGuangdong35.9 ± 2.6319.9 ± 1.2655.8 ± 3.89
8Allamanda schottiiGuangdong12.0 ± 1.0238.4 ± 0.9050.4 ± 1.92
9Areca triandraGuangdong0.67 ± 0.040.61 ± 0.041.28 ± 0.08
10Alpinia zerumbetGuangdong8.87 ± 0.516.11 ± 0.3415.0 ± 0.85
11Schefflera heptaphyllaGuangdong6.30 ± 0.1410.6 ± 0.4816.9 ± 0.62
12Ficus hispidaGuangdong4.93 ± 0.171.33 ± 0.116.26 ± 0.28
13Melaleuca leucadendronGuangdong68.1 ± 3.48146 ± 5.38214 ± 8.86
14Lagerstroemia speciosaGuangdong42.2 ± 1.79182 ± 7.89225 ± 9.68
15Acronychia pedunculataGuangdong4.31 ± 0.2610.5 ± 0.2914.8 ± 0.55
16Litsea rotundifoliaGuangdong17.6 ± 0.809.99 ± 0.4827.6 ± 1.28
17Lantana camaraGuangdong4.31 ± 0.191.81 ± 0.036.12 ± 0.22
18Dianella ensifoliaGuangdong2.66 ± 0.103.02 ± 0.185.68 ± 0.28
19Microcos paniculataGuangdong18.8 ± 0.359.71 ± 0.0828.5 ± 0.43
20Melastoma candidumGuangdong95.2 ± 5.8945.6 ± 2.88141 ± 8.77
21Diplospora dubiaGuangdong47.0 ± 2.41117 ± 6.56164 ± 8.97
22Dolichandrone caudafelinaGuangdong41.1 ± 2.395.64 ± 0.1346.7 ± 2.52
23Asparagus cochinchinensisHong Kong1.71 ± 0.062.02 ± 0.013.73 ± 0.07
24Psychotria asiaticaHong Kong41.3 ± 0.118.65 ± 0.6849.9 ± 0.79
25Lagerstroemia indicaHong Kong52.8 ± 2.3298.3 ± 0.32151 ± 2.64
26Nandina domesticaHong Kong51.1 ± 3.3859.0 ± 0.63110 ± 4.01
27Alpinia hainanensisHong Kong5.67 ± 0.317.66 ± 0.2113.3 ± 0.52
28Gordonia axillarisHong Kong74.4 ± 3.25106 ± 7.90180 ± 11.2
29Rhodomyrtus tomentosaHong Kong45.3 ± 2.7735.4 ± 1.1680.7 ± 3.93
30Breynia fruticosaHong Kong8.76 ± 0.685.50 ± 0.3114.3 ± 0.99
31Eurya chinensisHong Kong40.8 ± 2.5221.6 ± 0.2762.4 ± 2.79
32Duranta erectaHong Kong5.49 ± 0.409.20 ± 0.0514.7 ± 0.45
33Melastoma sanguineumHong Kong143 ± 2.00145 ± 8.44288 ± 10.4
34Eurya nitidaHong Kong83.3 ± 2.63345 ± 9.39428 ± 12.0
35Pyracantha fortuneanaHong Kong18.1 ± 0.146.09 ± 0.0924.2 ± 0.23
36Caryota mitisHong Kong35.6 ± 1.50138 ± 2.50174 ± 4.00
37Viburnum fordiaeJiangxi75.7 ± 2.0670.0 ± 1.16146 ± 3.22
38Xanthium sibiricumJiangxi1.47 ± 0.111.70 ± 0.013.17 ± 0.12
39Celtis sinesisJiangxi3.17 ± 0.301.67 ± 0.084.84 ± 0.38
40Solanum torvumJiangxi3.46 ± 0.221.78 ± 0.055.24 ± 0.27
41Melia azedarachJiangxi8.30 ± 0.397.07 ± 0.5015.4 ± 0.89
42Symplocos paniculataJiangxi6.51 ± 0.195.01 ± 0.0711.5 ± 0.26
43Solanum americanumJiangxi1.32 ± 0.062.21 ± 0.113.53 ± 0.17
44Ardisia crenataJiangxi13.7 ± 0.6020.1 ± 0.9033.9 ± 1.50
45Cinnamomum camphoraJiangxi10.2 ± 0.5910.1 ± 0.8020.3 ± 1.39
46Lagerstroemia indicaJiangxi118 ± 1.49137 ± 4.26254 ± 5.75
47Parthenocissus dalzieliiJiangxi20.3 ± 1.3266.5 ± 3.5386.8 ± 4.85
48Loropetalum chinenseJiangxi22.0 ± 0.3233.2 ± 2.1255.2 ± 2.44
49Rosa laevigataJiangxi86.9 ± 2.1858.2 ± 1.13145 ± 3.31
50Viburnum sempervirensJiangxi35.0 ± 1.0173.4 ± 4.01108 ± 5.02
51Diospyros kakiJiangxi1.88 ± 0.131.63 ± 0.093.51 ± 0.22
52Vernici afordiiJiangxi30.6 ± 1.3951.7 ± 2.1082.3 ± 3.49
53Swida austrosinensisJiangxi22.0 ± 0.6235.2 ± 0.5457.2 ± 1.16
54Hibiscus sabdariffaJiangxi8.68 ± 0.267.20 ± 0.2915.9 ± 0.55
55Rhus chinensisJiangxi21.3 ± 0.3038.5 ± 0.1859.8 ± 0.48
56Aralia armataJiangxi1.94 ± 0.041.90 ± 0.093.84 ± 0.13
The antioxidants in wild fruits are either water-soluble or fat-soluble. When determining total antioxidant activities of wild fruits, both water-soluble and fat-soluble components should be considered. In this study, the fat-soluble components of wild fruits were extracted with tetrahydrofuran, and their water-soluble components were extracted with a mixture of methanol-acetate acid-water [30]. The ferric reducing antioxidant power (FRAP) assay was used to evaluate antioxidant capacities of the 56 wild fruits. The FRAP assay, which is a simple, inexpensive and widely employed method for the evaluation of antioxidant capacity [20], is based on the capacity of antioxidants to reduce ferric(III) ions to ferrous(II) ions [31]. The FRAP values of the 56 wild fruits are given in Table 1.
As seen from the data in the table, the FRAP values varied from 0.67 ± 0.04 to 143 ± 2.00 μmol Fe(II)/g wet weight with a 213-fold difference for the water-soluble fractions, and eight wild fruits, namely Melastoma sanguineum [143 ± 2.00 µmol Fe(II)/g], Eucalyptus robusta [125 ± 2.49 µmol Fe(II)/g], Lagerstroemia indica [118 ± 1.49 µmol Fe(II)/g], Melastoma candidum [95.2 ± 5.89 µmol Fe(II)/g], Rosa laevigata [86.9 ± 2.18 µmol Fe(II)/g], Eurya nitida [83.3 ± 2.63 µmol Fe(II)/g], Viburnum fordiae [75.7 ± 2.06 µmol Fe(II)/g] and Gordonia axillaris [74.4 ± 3.25 µmol Fe(II)/g], had the highest antioxidant capacities, while Areca triandra had the lowest antioxidant capacity among the 56 wild fruits at 0.67 ± 0.04 μmol Fe(II)/g wet weight. For the fat-soluble fractions, the FRAP values varied from 0.61 ± 0.04 to 377 ± 3.92 μmol Fe(II)/g wet weight with a 618-fold difference, and eight wild fruits, Eucalyptus robusta [377 ± 3.92 µmol Fe(II)/g], Eurya nitida [345 ± 9.39 µmol Fe(II)/g)], Lagerstroemia speciosa [182 ± 7.89 µmol Fe(II)/g], Melaleuca leucadendron [146 ± 5.38 µmol Fe(II)/g], Melastoma sanguineum [145 ± 8.44 µmol Fe(II)/g)], Caryota mitis [138 ± 2.50 µmol Fe(II)/g], Lagerstroemia indica [137 ± 4.26 µmol Fe(II)/g] and Diplospora dubia [117 ± 6.56 µmol Fe(II)/g], had the highest antioxidant capacities, while Areca triandra again had the lowest antioxidant capacity among the 56 tested wild fruits with a value of 0.61 ± 0.04 μmol Fe(II)/g wet weight. For the combined value of water-soluble and fat-soluble fractions, the FRAP values varied from 1.28 ± 0.08 to 502 ± 6.41 μmol Fe(II)/g wet weight with a 392-fold difference, and eight wild fruits, Eucalyptus robusta [502 ± 6.41 µmol Fe(II)/g], Eurya nitida [428 ± 12.0 µmol Fe(II)/g], Melastoma sanguineum [288 ± 10.4 µmol Fe(II)/g], Lagerstroemia indica [254 ± 5.75 µmol Fe(II)/g], Lagerstroemia speciosa [225 ± 9.68 µmol Fe(II)/g], Melaleuca leucadendron [214 ± 8.86 µmol Fe(II)/g], Gordonia axillaris [180 ± 11.2 µmol Fe(II)/g] and Caryota mitis [174 ± 4.00 µmol Fe(II)/g], had the highest antioxidant capacities.
The antioxidant capacities of plant samples may be influenced by a lot of factors, such as the extraction solvent and test systems used, and cannot be fully described by any one single method. A reliable antioxidant evaluation protocol requires measurement of more than one property because most natural antioxidants are multifunctional, so it is essential to perform different antioxidant activity assessments to take into account the various mechanisms of antioxidant action [32]. Therefore, the Trolox equivalent antioxidant capacity (TEAC) assay was used to evaluate the free radical scavenging capacities of the 56 wild fruits. The TEAC assay is based on the ability of antioxidants to scavenge the ABTS•+ radical, and can measure antioxidant capacities of hydrophilic and lipophilic compounds in the same sample [33]. The TEAC assay is a simple, rapid and commonly used method for the evaluation of antioxidant capacity, and could offer reproducible results [21,22]. In the literature, natural and synthetic antioxidants, such as ascorbic acid, vitamin E, butylated hydroxytoluene, butylated hydroxyacetone and Trolox, were often used as positive controls [21,22,34,35]. In this study, Trolox was used, and the results were expressed as µmol Trolox/g wet weight of wild fruit. The TEAC values of the 56 wild fruits are given in Table 2.
Table
Table 2. The TEAC values (μmol Trolox/g) of 56 wild fruits.
Table 2. The TEAC values (μmol Trolox/g) of 56 wild fruits.
No.Scientific Name of PlantTEAC values (μmol Trolox/g)
Water-soluble fractionFat-soluble fractionTotal
1Mallotus apelta4.06 ± 0.1322.1 ± 0.8226.2 ± 0.95
2Smilax china24.4 ± 1.4698.2 ± 4.73123 ± 6.19
3Gardenia jasminoides2.26 ± 0.043.80 ± 0.086.06 ± 0.12
4Helicteres angustifolia5.99 ± 0.294.61 ± 0.2310.6 ± 0.52
5Eucalyptus robusta184 ± 2.14957 ± 5.791140 ± 7.93
6Ficus benjamina3.12 ± 0.105.78 ± 0.348.90 ± 0.44
7Ilex rotunda19.7 ± 0.1318.8 ± 0.3238.5 ± 0.45
8Allamanda schottii6.75 ± 0.2130.0 ± 1.7336.7 ± 1.94
9Areca triandra0.46 ± 0.026.05 ± 0.396.51 ± 0.41
10Alpinia zerumbet6.09 ± 0.2814.7 ± 0.6220.8 ± 0.90
11Schefflera heptaphylla2.02 ± 0.144.25 ± 0.116.27 ± 0.25
12Ficus hispida2.35 ± 0.182.83 ± 0.065.18 ± 0.24
13Melaleuca leucadendron138 ± 4.71323 ± 2.17461 ± 6.88
14Lagerstroemia speciosa58.0 ± 0.59264 ± 16.8322 ± 17.4
15Acronychia pedunculata2.73 ± 0.0819.2 ± 0.0521.9 ± 0.13
16Litsea rotundifolia11.5 ± 0.5616.8 ± 0.6528.3 ± 1.21
17Lantana camara1.75 ± 0.093.68 ± 0.175.43 ± 0.26
18Dianella ensifolia0.62 ± 0.033.70 ± 0.044.32 ± 0.07
19Microcos paniculata9.61 ± 0.1615.2 ± 0.0524.8 ± 0.21
20Melastoma candidum93.8 ± 4.4788.6 ± 1.00182 ± 5.47
21Diplospora dubia66.4 ± 0.2691.0 ± 0.80157 ± 1.06
22Dolichandrone caudafelina17.3 ± 0.5617.6 ± 0.0234.9 ± 0.58
23Asparagus cochinchinensis0.37 ± 0.013.68 ± 0.194.05 ± 0.20
24Psychotria asiatica36.1 ± 1.5833.6 ± 1.3669.7 ± 2.94
25Lagerstroemia indica73.2 ± 0.81119 ± 1.12192 ± 1.93
26Nandina domestica20.7 ± 0.2457.3 ± 0.5478.0 ± 0.78
27Alpinia hainanensis3.73 ± 0.1526.8 ± 1.6330.5 ± 1.78
28Gordonia axillaris79.4 ± 0.40265 ± 11.7344 ± 12.1
29Rhodomyrtus tomentosa29.7 ± 0.9448.7 ± 1.9378.4 ± 2.87
30Breynia fruticosa4.83 ± 0.1211.0 ± 0.4215.8 ± 0.54
31Eurya chinensis33.5 ± 0.2340.7 ± 0.7874.2 ± 1.01
32Duranta erecta1.76 ± 0.0819.0 ± 0.8820.8 ± 0.96
33Melastoma sanguineum130 ± 8.77275 ± 12.7404 ± 21.5
34Eurya nitida56.0 ± 2.52422 ± 11.6478 ± 14.1
35Pyracantha fortuneana7.40 ± 0.2518.1 ± 0.8825.5 ± 1.13
36Caryota mitis27.3 ± 0.35361 ± 10.4389 ± 10.8
37Viburnum fordiae88.0 ± 2.3254.8 ± 3.90143 ± 6.22
38Xanthium sibiricum12.0 ± 0.0111.4 ± 0.1423.3 ± 0.15
39Celtis sinesis11.3 ± 0.0812.3 ± 0.1123.6 ± 0.19
40Solanum torvum11.3 ± 0.0510.6 ± 0.1622.0 ± 0.21
41Melia azedarach10.5 ± 0.107.03 ± 0.2517.5 ± 0.35
42Symplocos paniculata10.4 ± 0.1014.0 ± 0.5224.4 ± 0.62
43Solanum americanum12.2 ± 0.0310.9 ± 0.1523.1 ± 0.18
44Ardisia crenata5.91 ± 0.1539.2 ± 2.0645.1 ± 2.21
45Cinnamomum camphora9.65 ± 0.118.14 ± 0.0917.8 ± 0.20
46Lagerstroemia indica64.1 ± 3.2645.0 ± 1.87109 ± 5.13
47Parthenocissus dalzielii19.6 ± 1.7688.6 ± 3.08108 ± 4.84
48Loropetalum chinense16.5 ± 1.4046.1 ± 0.5662.6 ± 1.96
49Rosa laevigata67.7 ± 0.3278.4 ± 4.51146 ± 4.83
50Viburnum sempervirens17.8 ± 0.5896.4 ± 1.56114 ± 2.14
51Diospyros kaki0.77 ± 0.032.95 ± 0.173.72 ± 0.20
52Vernici afordii18.2 ± 0.4392.4 ± 3.67111 ± 4.10
53Swida austrosinensis14.5 ± 0.2040.0 ± 2.7254.5 ± 2.92
54Hibiscus sabdariffa4.34 ± 0.169.50 ± 0.2313.8 ± 0.39
55Rhus chinensis29.0 ± 1.6086.4 ± 5.28115 ± 6.88
56Aralia armata0.91 ± 0.032.47 ± 0.123.38 ± 0.15
As seen from Table 2, the TEAC values varied from 0.37 ± 0.01 to 184 ± 2.14 μmol Trolox/g wet weight with a 496-fold difference for the water-soluble fractions, and eight wild fruits, Eucalyptus robusta [184 ± 2.14 μmol Trolox/g], Melaleuca leucadendron [138 ± 4.71 μmol Trolox/g], Melastoma sanguineum [130 ± 8.77 μmol Trolox/g], Melastoma candidum [93.8 ± 4.47 μmol Trolox/g], Viburnum fordiae [88.0 ± 2.32 μmol Trolox/g], Gordonia axillaris [79.4 ± 0.40 μmol Trolox/g], Lagerstroemia indica [73.2 ± 0.81 μmol Trolox/g] and Rosa laevigata [67.7 ± 0.32 μmol Trolox/g], had the highest free radical scavenging capacities, while in this assay Asparagus cochinchinensis with 0.37 ± 0.01 μmol Trolox/g wet weight showed the lowest free radical scavenging capacity among the tested wild fruits. For the fat-soluble fractions, the TEAC values varied from 2.47 ± 0.12 to 957 ± 5.79 μmol Trolox/g wet weight with a 387–fold difference, and eight wild fruits, Eucalyptus robusta [957 ± 5.79 μmol Trolox/g], Eurya nitida [422 ± 11.6 μmol Trolox/g], Caryota mitis [361 ± 10.4 μmol Trolox/g], Melaleuca leucadendron [323 ± 2.17 μmol Trolox/g], Melastoma sanguineum [275 ± 12.7 μmol Trolox/g], Gordonia axillaris [265 ± 11.7 μmol Trolox/g], Lagerstroemia speciosa [264 ± 16.8 μmol Trolox/g] and Lagerstroemia indica [119 ± 1.12 μmol Trolox/g], had the highest free radical scavenging capacities, whereas Aralia armata showed the lowest free radical scavenging capacity with 2.47 ± 0.12 μmol Trolox/g wet weight. For the combined value of the water-soluble and fat-soluble fractions, the TEAC values varied from 3.38 ± 0.15 to 1,140 ± 7.93 μmol Trolox/g wet weight with a 337–fold difference, and eight wild fruits, Eucalyptus robusta [1,140 ± 7.93 μmol Trolox/g], Eurya nitida [478 ± 14.1 μmol Trolox/g], Melaleuca leucadendron [461 ± 6.88 μmol Trolox/g], Melastoma sanguineum [404 ± 21.5 μmol Trolox/g], Caryota mitis [389 ± 10.8 μmol Trolox/g], Gordonia axillaris [344 ± 12.1 μmol Trolox/g], Lagerstroemia speciosa [322 ± 17.4 μmol Trolox/g] and Lagerstroemia indica [192 ± 1.93 μmol Trolox/g], had the highest free radical scavenging capacities.
Eight wild fruits, Eucalyptus robusta, Eurya nitida, Melastoma sanguineum, Melaleuca leucadendron, Lagerstroemia indica, Caryota mitis, Lagerstroemia speciosa and Gordonia axillaris, had the highest antioxidant activities among the 56 tested wild fruits based on the consideration of the combined results obtained by FRAP and TEAC assays. These wild fruits exhibited quite high antioxidant activities when compared with some vegetables and common edible fruits [21]. Their antioxidant capacities were also higher than those of other wild fruits reported in the literature [36]. Because of their high antioxidant activities, it could be speculated that these wild fruits could be beneficial for the diseases caused by oxidative stress, and might be developed into functional foods or drugs in the future.
The correlation between total antioxidant capacities obtained from FRAP and TEAC assays are shown in Figure 1. The results exhibited a positive linear correlation (R2 = 0.8263) between them. In addition, the results from water-soluble and fat-soluble fractions also showed a positive linear correlation between FRAP and TEAC values (Table 3, R2 = 0.7895 and 0.8334, respectively). These results suggested that antioxidant components in these wild fruits could reduce oxidants (such as ferric ions) and scavenge free radicals.
Molecules 15 08602 g001 550
Figure 1. Correlation between total antioxidant capacities measured by the FRAP and TEAC assays.
Figure 1. Correlation between total antioxidant capacities measured by the FRAP and TEAC assays.
Molecules 15 08602 g001
Table
Table 3. The relation of several evaluation parameters.
Table 3. The relation of several evaluation parameters.
ParameterWater-soluble fractionFat-soluble fractionTotal
TEAC value vsY = 1.0038X − 2.1363Y = 1.8305X − 8.6945Y = 1.6144X − 18.946
FRAP valueR2 = 0.7895R2 = 0.8334R2 = 0.8263
FRAP value vsY= 10.063X + 0.9349Y = 9.6777X − 12.752Y = 9.6354X − 10.3
Total phenolic contentR2 = 0.7956R2 = 0.9051R2 = 0.8916
TEAC value vsY = 11.978X − 6.6924Y = 18.997X − 40.128Y = 16.995X − 48.868
Total phenolic contentR2 = 0.8834R2 = 0.8675R2 = 0.8793

2.2. Total phenolic content of 56 wild fruits

The total phenolic contents of 56 wild fruits were estimated using the Folin–Ciocalteu method, which relies on the transfer of electrons from phenolic compounds to the Folin–Ciocalteu reagent in alkaline medium, and is a simple, rapid and reproducible method [22,37]. As seen from Table 4, the total phenolic contents varied from 0.06 ± 0.01 to 14.1 ± 0.56 mg GAE/g wet weight with a 235-fold difference for the water-soluble fractions, and eight wild fruits, Eucalyptus robusta [14.1 ± 0.56 mg GAE/g], Melastoma sanguineum [9.68 ± 0.10 mg GAE/g], Melaleuca leucadendron [9.28 ± 0.37 mg GAE/g], Melastoma candidum [8.56 ± 0.30 mg GAE/g], Gordonia axillaris [7.88 ± 0.44 mg GAE/g], Diplospora dubia [7.49 ± 0.42 mg GAE/g], Rosa laevigata [7.45 ± 0.28 mg GAE/g] and Eurya nitida [6.27 ± 0.36 mg GAE/g] showed the highest total phenolic contents, while Areca triandra had the lowest total phenolic content with 0.06 ± 0.01 mg GAE/g wet weight. For the fat-soluble fractions, the total phenolic contents varied from 0.38 ± 0.02 to 40.7 ± 2.49 mg GAE/g wet weight with a 107–fold difference, and eight wild fruits, Eucalyptus robusta [40.7 ± 2.49 mg GAE/g], Eurya nitida [28.8 ± 0.60 mg GAE/g], Caryota mitis [17.5 ± 0.71 mg GAE/g], Gordonia axillaris [16.7 ± 0.64 mg GAE/g], Melaleuca leucadendron [16.4 ± 1.22 mg GAE/g], Lagerstroemia speciosa [14.9 ± 0.32 mg GAE/g], Viburnum sempervirens [13.9 ± 0.12 mg GAE/g] and Melastoma sanguineum [13.6 ± 0.39 mg GAE/g], showed the highest total phenolic contents. Meanwhile, Diospyros kaki had the lowest total phenolic content with 0.38 ± 0.02 mg GAE/g wet weight. For the combined value of the water-soluble and fat-soluble fractions, the total phenolic contents varied from 0.49 ± 0.04 to 54.8 ± 3.05 mg GAE/g wet weight with a 112–fold difference, and eight wild fruits, Eucalyptus robusta [54.8 ± 3.05 mg GAE/g], Eurya nitida [35.0 ± 0.96 mg GAE/g], Melaleuca leucadendron [25.6 ± 1.59 mg GAE/g], Gordonia axillaris [24.6 ± 1.08 mg GAE/g], Melastoma sanguineum [23.3 ± 0.49 mg GAE/g], Caryota mitis [21.4 ± 0.85 mg GAE/g], Lagerstroemia speciosa [20.1 ± 0.35 mg GAE/g] and Viburnum sempervirens [18.5 ± 0.40 mg GAE/g], showed the highest total phenolic contents. The total phenolic contents of these eight wild fruits were very high when compared with other vegetables and common edible fruits reported in the literature [21]. Their total phenolic contents were also higher than those of wild fruits reported in the literature [36]. In addition, it should be pointed out that some non-phenolic reducing compounds, such as organic acids and sugars, could interfere the determination of total phenolic contents by the Folin–Ciocalteu method, which leads to an overvaluation of the phenolic content. Furthermore, different phenolics might present different responses with the Folin Ciocalteu reagent, such as gallic acid and rutin have similar behaviours, but several flavonoids present low absorption, which leads to an underestimation of various compounds [38,39,40,41].
Table
Table 4. The total phenolic contents (mg GAE/g) of 56 wild fruits.
Table 4. The total phenolic contents (mg GAE/g) of 56 wild fruits.
No.Scientific Name of PlantTotal phenolic contents (mg GAE/g)
Water-soluble fractionFat-soluble fractionTotal
1Mallotus apelta1.74 ± 0.106.11 ± 0.187.85 ± 0.28
2Smilax china4.80 ± 0.2211.0 ± 0.5615.8 ± 0.78
3Gardenia jasminoides0.72 ± 0.011.16 ± 0.061.88 ± 0.07
4Helicteres angustifolia1.07 ± 0.041.05 ± 0.062.12 ± 0.10
5Eucalyptus robusta 14.1 ± 0.5640.7 ± 2.4954.8 ± 3.05
6Ficus benjamina0.68 ± 0.020.95 ± 0.051.63 ± 0.07
7Ilex rotunda2.96 ± 0.063.36 ± 0.286.32 ± 0.34
8Allamanda schottii1.31 ± 0.047.37 ± 0.448.68 ± 0.48
9Areca triandra0.06 ± 0.010.43 ± 0.030.49 ± 0.04
10Alpinia zerumbet1.29 ± 0.102.91 ± 0.214.20 ± 0.31
11Schefflera heptaphylla0.54 ± 0.031.31 ± 0.061.85 ± 0.09
12Ficus hispida0.51 ± 0.020.75 ± 0.021.26 ± 0.04
13Melaleuca leucadendron9.28 ± 0.3716.4 ± 1.2225.6 ± 1.59
14Lagerstroemia speciosa5.22 ± 0.0314.9 ± 0.3220.1 ± 0.35
15Acronychia pedunculata0.46 ± 0.013.13 ± 0.043.59 ± 0.05
16Litsea rotundifolia2.06 ± 0.123.79 ± 0.305.85 ± 0.42
17Lantana camara0.62 ± 0.020.92 ± 0.011.54 ± 0.03
18Dianella ensifolia0.26 ± 0.021.15 ± 0.021.41 ± 0.04
19Microcos paniculata2.68 ± 0.063.60 ± 0.136.28 ± 0.19
20Melastoma candidum8.56 ± 0.307.61 ± 0.6816.2 ± 0.98
21Diplospora dubia7.49 ± 0.4210.9 ± 0.1218.4 ± 0.54
22Dolichandrone caudafelina3.15 ± 0.202.48 ± 0.025.63 ± 0.22
23Asparagus cochinchinensis0.29 ± 0.010.61 ± 0.030.90 ± 0.04
24Psychotria asiatica3.55 ± 0.243.73 ± 0.157.28 ± 0.39
25Lagerstroemia indica5.42 ± 0.1712.0 ± 0.0717.4 ± 0.24
26Nandina domestica4.25 ± 0.207.72 ± 0.3912.0 ± 0.59
27Alpinia hainanensis0.78 ± 0.022.00 ± 0.092.78 ± 0.11
28Gordonia axillaris7.88 ± 0.4416.7 ± 0.6424.6 ± 1.08
29Rhodomyrtus tomentosa2.71 ± 0.074.07 ± 0.136.78 ± 0.20
30Breynia fruticosa1.22 ± 0.041.83 ± 0.073.05 ± 0.11
31Eurya chinensis3.42 ± 0.053.85 ± 0.067.27 ± 0.11
32Duranta erecta0.43 ± 0.021.80 ± 0.022.23 ± 0.04
33Melastoma sanguineum9.68 ± 0.1013.6 ± 0.3923.3 ± 0.49
34Eurya nitida6.27 ± 0.3628.8 ± 0.6035.0 ± 0.96
35Pyracantha fortuneana1.31 ± 0.042.03 ± 0.073.34 ± 0.11
36Caryota mitis3.87 ± 0.1417.5 ± 0.7121.4 ± 0.85
37Viburnum fordiae5.60 ± 0.4410.6 ± 0.2016.2 ± 0.64
38Xanthium sibiricum0.20 ± 0.010.45 ± 0.020.65 ± 0.03
39Celtis sinesis0.42 ± 0.020.65 ± 0.031.07 ± 0.05
40Solanum torvum0.48 ± 0.010.49 ± 0.040.97 ± 0.05
41Melia azedarach0.76 ± 0.031.44 ± 0.052.20 ± 0.08
42Symplocos paniculata0.57 ± 0.031.38 ± 0.011.95 ± 0.04
43Solanum americanum0.16 ± 0.010.47 ± 0.010.63 ± 0.02
44Ardisia crenata0.85 ± 0.032.57 ± 0.133.42 ± 0.16
45Cinnamomum camphora0.92 ± 0.042.28 ± 0.093.20 ± 0.13
46Lagerstroemia indica4.08 ± 0.028.01 ± 0.2812.1 ± 0.30
47Parthenocissus dalzielii3.93 ± 0.2711.8 ± 0.6315.8 ± 0.90
48Loropetalum chinense2.43 ± 0.125.08 ± 0.317.51 ± 0.43
49Rosa laevigata7.45 ± 0.287.34 ± 0.2114.8 ± 0.49
50Viburnum sempervirens4.62 ± 0.2813.9 ± 0.1218.5 ± 0.40
51Diospyros kaki0.15 ± 0.010.38 ± 0.020.53 ± 0.03
52Vernici afordii2.98 ± 0.159.24 ± 0.0412.2 ± 0.19
53Swida austrosinensis2.09 ± 0.164.81 ± 0.246.90 ± 0.40
54Hibiscus sabdariffa0.60 ± 0.011.45 ± 0.052.05 ± 0.06
55Rhus chinensis4.74 ± 0.4212.4 ± 1.0717.2 ± 1.49
56Aralia armata0.24 ± 0.010.71 ± 0.010.95 ± 0.02

2.3. Correlation between antioxidant capacities and total phenolic content

The correlation between total antioxidant capacities and the total phenolic content of the 56 tested wild fruits is shown in Figure 2. The results showed a positive linear correlation between the antioxidant capacities and total phenolic content (R2 = 0.8916 and 0.8793 for the results from the FRAP and TEAC assays, respectively). In addition, the results from the water-soluble fractions showed a positive linear correlation between the antioxidant capacity and total phenolic content (Table 3, R2 = 0.7956 and 0.8834 for the results from the FRAP and TEAC assays, respectively). Furthermore, the results from the fat-soluble fractions also showed a positive linear correlation between the antioxidant capacity and total phenolic content (Table 3, R2 = 0.9051 and 0.8675 for the results from the FRAP and TEAC assays, respectively). These results indicated that the phenolic compounds could be the main contributor of the antioxidant activities of these wild fruits. This result was in agreement with many previous studies [26,27].
Molecules 15 08602 g002 550
Figure 2. Correlation between the antioxidant capacities and total phenolic content. Antioxidant capacities were measured by the FRAP assay (A) and TEAC assay (B), respectively. GAE: gallic acid equivalents.
Figure 2. Correlation between the antioxidant capacities and total phenolic content. Antioxidant capacities were measured by the FRAP assay (A) and TEAC assay (B), respectively. GAE: gallic acid equivalents.
Molecules 15 08602 g002
Comparison of FRAP value, TEAC value and total phenolic content between water-soluble fraction and fat-soluble fraction showed that there was a significant difference in TEAC values (P = 0.010 < 0.05) and total phenolic contents (P = 0.005 < 0.05), but no significant difference in FRAP values (P = 0.954 > 0.05). The fat-soluble fractions possessed stronger scavenging free radical activity and higher phenolic content than the water-soluble fractions. In addition, comparison of FRAP value, TEAC value and total phenolic content of the wild fruits among different sampling locations: Guangdong province, Hong Kong and Jiangxi province, showed that there was no significant difference in FRAP values (P = 0.257 > 0.05), TEAC values (P = 0.200 > 0.05) and total phenolic contents (P = 0.226 > 0.05). Furthermore, three wild fruits, Gordonia axillaris, Rhodomyrtus tomentosa and Caryota mitis, were simultaneously collected both in Guangdong province and Hong Kong. Although their FRAP value, TEAC value and total phenolic content were different (data not shown), there was no significant difference in the three parameters. Although these wild fruits grow in different regions of South China, three regions all belong to the sub-tropical climate.

3. Experimental

3.1. Chemicals and wild fruits

Folin–Ciocalteu’s phenol reagent, 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), 2,2'-azinobis(3-ethylbenothiazoline-6-sulfonic acid) diammonium salt (ABTS) and gallic acid were purchased from Sigma–Aldrich (St. Louis, MO, USA). Iron (III) chloride hexahydrate, iron (II) sulfate heptahydrate, acetic acid, sodium acetate, potassium persulphate, tetrahydrofuran and sodium carbonate were obtained from Tianjing Chemical Factory (Tianjing, China). Ethanol, methanol and hydrochloric acid were obtained from Kelong Chemical Factory (Chengdu, China). All chemicals used in the experiments were of analytical grade, and deionized water was used throughout. Fifty-six wild fruit samples were collected from various locations in South China, and are authenticated by the botanist Dr. Xin-Sheng Qin from the College of Forestry, South China Agricultural University. The scientific names of the plants and sampling sites of the wild fruit are shown in Table 1.

3.2. Sample preparation

The wild fruits were washed with deionized water to remove dirt on their peels, and were given an airing at room temperature. Then, the wild fruits were ground into fine particles/slurry with a special grinder for herbal medicines. A weighed amount (about 1.00 g) of these particles was extracted with tetrahydrofuran (10 mL) at room temperature for 30 min in a shaking water bath according to the literature [30]. The sample was centrifuged at 3,500 rpm for 30 min, and the supernatant was collected. The residue was extracted with the same solvent twice, and the supernatants were combined, which would be used for the evaluation of antioxidant capacity and total phenolic content. Subsequently, the residue was extracted with a mixture of methanol-acetic acid-water (10 mL, 50:3.7:46.3, v/v). The extraction procedure was repeated twice, and the supernatants were combined for the evaluation of antioxidant capacity and total phenolic content.

3.3. Ferric-reducing antioxidant power (FRAP) assay

The FRAP assay was carried out according to the procedure described in the literature with minor modifications [31,42]. Briefly, the FRAP reagent was prepared from sodium acetate buffer (300 mM, pH 3.6), 10 mM TPTZ solution (40 mM HCl as solvent) and 20 mM iron (III) chloride solution in a volume ratio of 10:1:1, respectively. The FRAP reagent was prepared freshly daily and warmed to 37 °C in a water bath before use. One hundred microliters of the diluted sample was added to a portion of the FRAP reagent (3 mL). After 4 min, the absorbance of the reaction mixture was measured at 593 nm using a Shimadzu UV-2450 ultraviolet-visible spectrophotometer (Japan). The standard curve was constructed using FeSO4 solution, and the results were expressed as µmol Fe(II)/g wet weight of wild fruit.

3.4. Trolox equivalent antioxidant capacity (TEAC) assay

The TEAC assay was carried out according to the method established in the literature with slight modifications [33]. Briefly, the ABTS•+ stock solution was prepared from 7 mM ABTS and 2.45 mM potassium persulfate in a volume ratio of 1:1, and then incubated in the dark for 16 h at room temperature and used within 2 days. The ABTS•+ working solution was prepared by diluting the stock solution with ethanol to an absorbance of 0.70 ± 0.05 at 734 nm. All samples were diluted approximately to provide 20–80% inhibition of the blank absorbance. One hundred microliters of the diluted sample was mixed with ABTS•+ working solution (3.8 mL) and after 6 min of incubation at room temperature, the absorbance of the reaction mixture was measured at 734 nm, and the percent of inhibition of absorbance at 734 nm was calculated. Trolox was used as a reference standard, and the results were expressed as µmol Trolox/g wet weight of wild fruit.

3.5. Determination of total phenolic content

Total phenolic contents were determined according to the literature [22,37]. Briefly, the diluted sample (0.50 mL) was added to 1:10 diluted Folin–Ciocalteu reagent (2.5 mL). After 4 min, saturated sodium carbonate solution (about 75 g/L, 2 mL) was added. The absorbance of the reaction mixture was measured at 760 nm after incubation for 2 h at room temperature. Gallic acid was used as a reference standard, and the results were expressed as milligram gallic acid equivalent (mg GAE)/g wet weight of wild fruit.

3.6. Statistical analysis

All the experiments were performed in triplicate, and the results were expressed as mean ± SD (standard deviation). Statistical analysis was performed using SPSS 13.0 and Excel 2003. The difference was considered significant at p < 0.05.

4. Conclusions

The antioxidant activities and total phenolic contents of 56 wild fruits collected from South China were evaluated. Generally, these wild fruits had high antioxidant capacities and total phenolic contents. A significant correlation between the FRAP value and the TEAC value suggested that antioxidant components in these wild fruits were capable of reducing oxidants and scavenging free radicals. A high correlation between antioxidant capacity and total phenolic content indicated that phenolic compounds could be the main contributors to the antioxidant activity of these wild fruits. The fruits of Eucalyptus robusta, Eurya nitida, Melastoma sanguineum, Melaleuca leucadendron, Lagerstroemia indica, Caryota mitis, Lagerstroemia speciosa and Gordonia axillaris possessed the highest antioxidant capacities and total phenolic contents among the wild fruits tested. These fruits could be potential rich resources of natural antioxidants, and could be developed into functional foods or drug for the prevention and treatment of diseases caused by oxidative stress. In the future, the specific components with high antioxidant capacities in these wild fruits should be isolated and identified, and explored for their health effects against oxidative stress.

Acknowledgements

This research was supported by the Hundred-Talents Scheme of Sun Yat-Sen University. The useful suggestion and technical assistance from Wen-Qing Lu and Bin Zhang is highly appreciated.
  • Samples Availability: The wild fruit samples are available from the authors.

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MDPI and ACS Style

Fu, L.; Xu, B.-T.; Xu, X.-R.; Qin, X.-S.; Gan, R.-Y.; Li, H.-B. Antioxidant Capacities and Total Phenolic Contents of 56 Wild Fruits from South China. Molecules 2010, 15, 8602-8617. https://doi.org/10.3390/molecules15128602

AMA Style

Fu L, Xu B-T, Xu X-R, Qin X-S, Gan R-Y, Li H-B. Antioxidant Capacities and Total Phenolic Contents of 56 Wild Fruits from South China. Molecules. 2010; 15(12):8602-8617. https://doi.org/10.3390/molecules15128602

Chicago/Turabian Style

Fu, Li, Bo-Tao Xu, Xiang-Rong Xu, Xin-Sheng Qin, Ren-You Gan, and Hua-Bin Li. 2010. "Antioxidant Capacities and Total Phenolic Contents of 56 Wild Fruits from South China" Molecules 15, no. 12: 8602-8617. https://doi.org/10.3390/molecules15128602

APA Style

Fu, L., Xu, B. -T., Xu, X. -R., Qin, X. -S., Gan, R. -Y., & Li, H. -B. (2010). Antioxidant Capacities and Total Phenolic Contents of 56 Wild Fruits from South China. Molecules, 15(12), 8602-8617. https://doi.org/10.3390/molecules15128602

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