Caspase-14 is an anti-apoptotic protein targeting apoptosis-inducing factor in lung adenocarcinomas

Authors:
- Hsin-Yuan Fang
- Chih-Yi Chen
- Min-Fa Hung
- Yi-Ting Hsiao
- Ting-Chieh Chiang
- Tze-Yi Lin
- Hui-Wen Chang
- Kuan-Chih Chow
- Wen-Je Ko
View Affiliations

Affiliations: Graduate Institute of Clinical Medicine, National Taiwan University School of Medicine, Taipei, Taiwan, R.O.C., Graduate Institute of Biomedical Sciences, National Chung Hsing University, Taichung, Taiwan, R.O.C, Graduate Institute of Clinical Medicine, National Taiwan University School of Medicine, Taipei, Taiwan, R.O.C
Published online on: April 29, 2011 https://doi.org/10.3892/or.2011.1292
Pages: 359-369

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Using apoptosis-inducing factor protein as bait in a yeast hybrid assay to screen protein libraries, we identified three proteins that interacted with apoptosis-inducing factor: human homolog of yeast Rad23 protein A (hHR23A), microsomal glutathione S-transferase and caspase-14 (casp-14). In this study, we investigated the expression and function of casp-14 in lung adenocarcinomas (LADC). Our results showed that monoclonal antibodies were specific to casp-14, and that casp-14 was highly expressed in LADC. Casp-14 overexpression correlated with tumor stage, cell differentiation and lymphovascular involvement, suggesting that casp-14 was associated with tumor cell growth and metastatic potential. In vitro, casp-14 interacted with apoptosis-inducing factor, and silencing of casp-14 expression reduced cisplatin resistance. Our data suggest that casp-14 is an anti-apoptotic protein targeting apoptosis-inducing factor and increases cisplatin resistance in LADC cells.