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Differentiation of Human Endometrial Stem Cells into Schwann Cells in Fibrin Hydrogel as 3D Culture

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An Erratum to this article was published on 28 January 2016

Abstract

Human endometrial stem cells (hEnSCs) are a new source of adult multipotent stem cells with the ability of differentiation into many cell lineages. Many stem cell sources are desirable for differentiation into Schwann cells. Schwann-like cells derived from hEnSCs may be one of the ideal alternative cell sources for Schwann cell generation. In this study, for differentiation of hEnSCs into Schwann cells, hEnSCs were induced with RA/FSK/PDGF-AA/HRG as an induction medium for 14 days. The cells were cultured in a tissue culture plate (TCP) and fibrin gel matrix. The viability of cultured cells in the fibrin gel and TCP was analyzed with 3-[4,5-dimethyl-2-thia-zolyl]-2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay for 7 days. The attachment of cells was analyzed with SEM and DAPI staining. The expression of S100 and P75 as Schwann cell markers was evaluated by immunocytochemistry and quantitative real-time PCR (RT-PCR). The evaluation of the MTT assay and gene expression showed that the survival rate and differentiation of hEnSCs into Schwann cells in the fibrin gel were better than those in the TCP group. These results suggest that human EnSCs can be differentiated into Schwann cells in the fibrin gel better than in the TCP, and the fibrin gel might provide a suitable three-dimensional (3D) scaffold for clinical applications for cell therapy of the nervous system.

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Acknowledgments

This research has been supported by Iran National Science Foundation (INSF) grant number 91042417.

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Authors and Affiliations

  1. Brain and Spinal Cord Injury Research Center (BASIR), Tehran University of Medical Sciences, Keshavarz Boulevard, Gharib Street, 6114185, Tehran, Iran

    Neda Bayat & Jafar Ai

  2. Department of Tissue Engineering and Applied Cell Sciences, Faculty of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Somayeh Ebrahimi-Barough, Mohammad Mehdi Mokhtari Ardakan, Niloofar Babaloo & Jafar Ai

  3. School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Arman Ai

  4. Department of Genetics, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Ahmadreza Kamyab

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  1. Neda Bayat
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  2. Somayeh Ebrahimi-Barough
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Correspondence to Somayeh Ebrahimi-Barough or Jafar Ai.

Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/s12035-016-9730-8.

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Bayat, N., Ebrahimi-Barough, S., Ardakan, M.M.M. et al. Differentiation of Human Endometrial Stem Cells into Schwann Cells in Fibrin Hydrogel as 3D Culture. Mol Neurobiol 53, 7170–7176 (2016). https://doi.org/10.1007/s12035-015-9574-7

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  • DOI: https://doi.org/10.1007/s12035-015-9574-7

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