Abstract
Interleukin (IL)-27 is an IL-12 family cytokine and exerts a critical role in immune regulation in the context of infection, autoimmunity, and angiogenesis. In this study, we aimed to investigate the possible pathophysiological role of IL-27 and vascular endothelial growth factor (VEGF) in ankylosing spondylitis (AS). One hundred and forty AS patients and 90 healthy controls were included in the current study. The levels of IL-27 and VEGF in serum and synovial fluid (SF) samples were measured by enzyme-linked immunosorbent assay. Erythrocyte sedimentation rate, C-reactive protein, and human leukocyte antigen (HLA)-B27 were measured by standard laboratory techniques. Disease activity in AS was scored with Bath Ankylosing Spondylitis Disease Activity Index (BASDAI). Hip involvement, peripheral arthritis, and eye involvement were also recorded. The serum levels of IL-27 were remarkably higher in AS patients than healthy groups and significantly correlated with serum levels of VEGF. Furthermore, the serum levels of IL-27 were correlated with BASDAI independent of other markers of inflammation. Elevated serum levels of IL-27 and VEGF were detected in AS patients with peripheral arthritis and HLA-B27 positive. The SF levels of IL-27 and VEGF were significantly higher than serum levels in AS patients with peripheral arthritis. By contrast, levels of IL-27 and VEGF were not increased in AS patients with hip involvement and eye involvement. IL-27 may regulate the immunological or inflammatory process of AS.
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References
Pflanz S, Timans JC, Cheung J, Rosales R, Kanzler H, Gilbert J et al (2002) IL-27, a heterodimeric cytokine composed of EBI3 and p28 protein, induces proliferation of naive CD4(+) T cells. Immunity 16:779–790
Yoshida H, Yoshiyuki M (2008) Regulation of immune responses by interleukin-27. Immunol Rev 226:234–247
Stumhofer JS, Laurence A, Wilson EH, Huang E, Tato CM, Johnson LM, Villarino AV, Huang Q, Yoshimura A, Sehy D, Saris CJ, O’shea JJ et al (2006) Interleukin 27 negatively regulates the development of interleukin 17-producing T helper cells during chronic inflammation of the central nervous system. Nat Immunol 7:937–945
Batten M, Li J, Yi S, Kljavin NM, Danilenko DM, Lucas S, Lee J, de Sauvage FJ, Ghilardi N (2006) Interleukin 27 limits autoimmune encephalomyelitis by suppressing the development of interleukin 17-producing T cells. Nat Immunol 7:929–936
Mei Y, Pan F, Gao J, Ge R, Duan Z, Zeng Z et al (2011) Increased serum IL-17 and IL-23 in the patient with ankylosing spondylitis. Clin Rheumatol 30(2):269–273
Drouart M, Saas P, Billot M, Cedoz JP, Tiberghien P, Wendling D et al (2003) High serum vascular endothelial growth factor correlates with disease activity of spondylarthropathies. Clin Exp Immunol 132(1):158–162
Pickens SR, Chamberlain ND, Volin MV, Mandelin AM 2nd, Agrawal H, Matsui M, Yoshimoto T, Shahrara S (2011) Local expression of interleukin-27 ameliorates collagen-induced arthritis. Arthritis Rheum 63:2289–2298
Baek SH, Lee SG, Park YE, Kim GT, Kim CD, Park SY (2012) Increased synovial expression of IL-27 by IL-17 in rheumatoid arthritis. Inflamm Res 61(12):1339–1345
Van der Linden S, Valkenburg HA, Cats A (1984) Evaluation of diagnostic criteria for ankylosing spondylitis. A proposal for modification of the New York criteria. Arthritis Rheum 27(4):361–368
Garrett S, Jenkinson T, Kennedy LG et al (1994) A new approach to defining disease status in ankylosing spondylitis: the Bath Ankylosing Spondylitis Disease Activity Index. J Rheumatol 21(12):2286–2291
Niedbala W, Cai B, Wei X, Patakas A, Leung BP, McInnes IB, Liew FY (2008) Interleukin 27 attenuates collagen-induced arthritis. Ann Rheum Dis 67:1474–1479
Shen H, Xia L, Xiao W, Lu J (2011) Increased levels of interleukin-27 in patients with rheumatoid arthritis. Arthritis Rheum 63(3):860–861
Weber AJ, De Bandt M (2000) Angiogenesis: general mechanisms and implications for rheumatoid arthritis. Joint Bone Spine 67:366–383
Jones PBB, Makki RJ, Weiss JB (1994) Endothelial cell stimulating angiogenesis factor—a new biological marker for disease activity in ankylosing spondylitis? Br J Rheumatol 33:332–335
Lu J, Kasama T, Kobayashi K, Yoda Y, Shiozawa F, Hanyuda M et al (2000) Vascular endothelial growth factor expression and regulation of murine collagen-induced arthritis. J Immunol 164(11):5922–5927
Goldberg R, Wildbaum G, Zohar Y, Maor G, Karin N (2004) Suppression of ongoing adjuvant-induced arthritis by neutralizing the function of the p28 subunit of IL-27. J Immunol 173:1171–1178
Tanida S, Yoshitomi H, Ishikawa M, Kasahara T, Murata K, Shibuya H et al (2011) IL-27-producing CD14(+) cells infiltrate inflamed joints of rheumatoid arthritis and regulate inflammation and chemotactic migration. Cytokine 55:237–244
Xia L, Shen H, Zhao L, Lu J (2012) Elevated levels of interleukin-27 in patients with Sjögren’s syndrome. Scand J Rheumatol 41(1):73–74
Acknowledgments
This work was supported by National Nature Science Foundation of China under Grant: No. 81172867.
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All authors have no actual or potential conflicts of interest with other people or organizations within 3 years of initiating the work presented here.
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Lin, Tt., Lu, J., Qi, Cy. et al. Elevated serum level of IL-27 and VEGF in patients with ankylosing spondylitis and associate with disease activity. Clin Exp Med 15, 227–231 (2015). https://doi.org/10.1007/s10238-014-0281-x
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DOI: https://doi.org/10.1007/s10238-014-0281-x