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Alleviation of ultraviolet-C-induced oxidative damage through overexpression of cytosolic ascorbate peroxidase

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Abstract

Experiments were conducted to investigate the relationship between ultraviolet (UV) C-induced oxidative damage and the activity of ascorbate peroxidase (APX), using transgenic tobacco (Nicotiana tabacum L. cv. Petit Havana) plants overexpressing cytosolic APX gene (apx1). Transgenic plants having 2.3 fold higher total APX activity, as compared to the wild type plants, showed normal morphological characters. Exposure of 70-day-old plants to fixed intensity UV-C radiation caused an increase in the malondialdehyde (MDA) content in wild type as well as transgenic plants. However, the wild type plants showed significantly higher (p < 0.05) lipid peroxidation as compared to the transgenic plants. Higher proline accumulation was recorded in transgenic plants as compared to the wild type plants, after 24 hours of UV-C exposure. Although the ascorbate content decreased continuously with increasing exposure to UV-C radiation, yet the wild type plants exhibited higher ascorbate levels than the transgenic plants. A marked difference in H2O2 content, between the wild type and transgenic plants, was consistently observed up to 20 hours of UV-C exposure. A direct correlation of ascorbate, MDA and H2O2 levels was recorded with the extent of oxidative stress, signifying that these could be used as potential bio-marker molecules for oxidative stress. The results clearly demonstrate that overexpression of cytosolic APX can protect tobacco plants from UV-C-induced oxidative damage.

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Abbreviations

APX:

ascorbate peroxidase

MDA:

malondialdehyde

ROS:

reactive oxygen species

UV:

ultraviolet

References

  • Alexieva V., Sergio I., Mapelli S. & Karanov E. 2001. The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant Cell Environ. 24: 1337–1344.

    Article  CAS  Google Scholar 

  • Asada K. 1994. Production and action of active oxygen species in photosynthetic tissues, pp. 77–104. In: Foyer C.H. & Mullineaux P.M. (eds), Causes of Photoxidative Stress and Amelioration of Defense Systems in Plants. CRC Pres, Boca Raton, FL.

    Google Scholar 

  • Asada K. 2000. The water-water cycle as alternative photon and electron sinks. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 355: 1419–1431.

    Article  PubMed  CAS  Google Scholar 

  • Bates L.S., Waldsen R.O. & Teare I.D. 1973. Rapid determination of free proline for water stress studies. Plant Soil 39: 205–207.

    Article  CAS  Google Scholar 

  • Blokhina O., Virolainen E. & Fagerstedt K.V. 2003. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann. Bot. 91: 179–194.

    Article  PubMed  CAS  Google Scholar 

  • Bradford M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.

    Article  PubMed  CAS  Google Scholar 

  • Chen G.X. & Asada K. 1989. Ascorbate peroxidase in tea leaves: occurrence of two isozymes and the differences in their enzymatic and molecular properties. Plant Cell Physiol. 30: 987–998.

    CAS  Google Scholar 

  • Davletova S., Rizhsky L., Liang H., Shengqiang H., Oliver D.J., Coutu J., Shulaev V., Schlauch K. & Mittler R. 2005. Cytosolic ascorbate peroxidase-1 is a central component of the reactive oxygen gene network of Arabidopsis. Plant Cell 17: 268–281.

    Article  PubMed  CAS  Google Scholar 

  • Du Y., Jiang P., Wang B. & Shi Y. 2003. Effects of ultraviolet-C irradiation on membrane lipid peroxidation and activity of PS II electron transport in chloroplasts of Taxus cuspidata needles. Chin. J. Appl. Ecol. 14: 1218–1222.

    CAS  Google Scholar 

  • Du Y.J. & Jin Y.H. 2000. Effect of far-ultraviolet radiation on lipid peroxidation and inherent protection system in seedlings of Taxus cuspidate. Chin. J. Appl. Ecol. 11: 660–664.

    CAS  Google Scholar 

  • Foyer C.H., Lelandais M. & Kunert K.J. 1994. Photooxidative stress in plants. Physiol. Plant. 92: 696–717.

    Article  CAS  Google Scholar 

  • Heath R.L. & Packer L. 1968. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 125: 189–198.

    Article  PubMed  CAS  Google Scholar 

  • Ishikawa T., Takeda T., Shigeoka S., Hirayama O. & Mitsunaga T. 1993. Hydrogen peroxide generation in organelles of Euglena gracilis. Phytochemistry 33: 1297–1299.

    Article  CAS  Google Scholar 

  • Jaleel C.A., Riadh K., Gopi R., Manivannan P., Ines J., Al-Juburi H.J., Zhao C.X., Hong-Bo S. & Panneerselvam R. 2009. Antioxidant defense responses: physiological plasticity in higher plants under abiotic constraints. Acta Physiol. Plant. 31: 427–436.

    Article  Google Scholar 

  • Joshi P., Saxena S.C. & Arora S. 2011. Characterization of Brassica juncea antioxidant potential under salinity stress. Acta Physiol. Plant. 33: 811–822.

    Article  CAS  Google Scholar 

  • Mano J., Ohno C., Domae Y. & Asada K. 2001. Chloroplastic ascorbate peroxidase is the primary target of methyl viologeninduced photo oxidative stress in spinach leaves: its relevance to monodehydroascorbate radical detected with in vivo ESR. Biochim. Biophys. Acta 1504: 275–287.

    Article  PubMed  CAS  Google Scholar 

  • Matysik J., Alia, Bhalu B. & Mohanty P. 2002. Molecular mechanisms of quenching of reactive oxygen species by proline under stress in plants. Curr. Sci. 82: 525–532.

    CAS  Google Scholar 

  • Pitcher L.H., Repetti P. & Zilinskas B.A. 1994. Overproduction of ascorbate peroxidase protects transgenic tobacco plants against oxidative stress. Plant Physiol. 105(Suppl. 1): 116 (Abstract No. 623).

    Google Scholar 

  • Polle A. 2001. Dissecting the superoxide dismutase-ascorbateglutathione-pathway in chloroplasts by metabolic modeling. Computer simulations as a step towards flux analysis. Plant Physiol. 126: 445–462.

    Article  PubMed  CAS  Google Scholar 

  • Prasad S.M., Dwivedi R., Zeesham & Singh R. 2004. UV-B and cadmium induced changes in pigments, photosynthetic electron transport activity, antioxidant levels and antioxidative enzyme activities of Riccia sp. Acta Physiol. Plant. 26: 423–430.

    Article  CAS  Google Scholar 

  • Rosales M.A., Ruiz J.M., Hernández J., Soriano T., Castilla N. & Romero L. 2006. Antioxidant content and ascorbate metabolism in cherry tomato exocarp in relation to temperature and solar radiation. J. Sci. Food Agric. 86: 1545–1551.

    Article  CAS  Google Scholar 

  • Sreenivasulu N., Sopory S.K. & Kavi-Kishor P.B. 2007. Deciphering the regulatory mechanisms of abiotic stress tolerance in plants by genomic approaches. Gene 388: 1–13.

    Article  PubMed  CAS  Google Scholar 

  • Takeda T., Yokota A. & Shigoka S. 1995. Resistance of photosynthesis to hydrogen peroxide in algae. Plant Cell Physiol. 36: 1089–1095.

    CAS  Google Scholar 

  • Thimmaiah S.K. 1999. Standard Methods of Biochemical Analysis. Kalyani Publishers, India, 112 pp.

    Google Scholar 

  • Wang J., Zhang H. & Allen R.D. 1999. Over expression an Arabidopsis peroxisomal ascorbate peroxidase gene in tobacco increases protection against oxidative stress. Plant Cell Physiol. 40: 725–732.

    PubMed  CAS  Google Scholar 

  • Yang F., Xiao X., Zhang S., Korpelainen H. & Li C. 2009. Salt stress responses in Populus cathayana Rehder. Plant Sci. 176: 669–677.

    Article  CAS  Google Scholar 

  • Yannarelli G.G., Noriega G.O., Batlle A. & Tomaro M.L. 2006. Heme oxygenase up-regulation in ultraviolet-B irradiated soybean plants involves reactive oxygen species. Planta 224: 1154–1162.

    Article  PubMed  CAS  Google Scholar 

  • Zacchini M. & de Agazio M. 2004. Spread of oxidative damage and antioxidative response through cell layers of tobacco callus after UV-C treatment. Plant Physiol. Biochem. 42: 445–450.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, GB Pant University of Agriculture & Technology, Pantnagar, 263145, Uttarakhand, India

    Saurabh C. Saxena & Pankaj K. Joshi

  2. Department of Plant Physiology, Institute of Biology, Humboldt University, Berlin, Germany

    Bernhard Grimm

  3. Department of Molecular Biology & Genetic Engineering, GB Pant University of Agriculture & Technology, Pantnagar, 263145, Uttarakhand, India

    Sandeep Arora

Authors
  1. Saurabh C. Saxena
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  2. Pankaj K. Joshi
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  3. Bernhard Grimm
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  4. Sandeep Arora
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Correspondence to Sandeep Arora.

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Saxena, S.C., Joshi, P.K., Grimm, B. et al. Alleviation of ultraviolet-C-induced oxidative damage through overexpression of cytosolic ascorbate peroxidase. Biologia 66, 1052–1059 (2011). https://doi.org/10.2478/s11756-011-0120-4

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  • DOI: https://doi.org/10.2478/s11756-011-0120-4

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