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RP-LC for Determination of Plastochromanol, Tocotrienols and Tocopherols in Plant Oils

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Abstract

An isocratic high performance liquid chromatographic method, with the application of C18 and C30 reverse-phase column and fluorescence detection, is described for the analysis of plastochromanol, tocotrienols and tocopherols in plant seed oils. The solvent systems have been optimized to obtain high resolution for all tocochromanols and relatively short analysis time. The use of reverse-phase columns for plastochromanol analysis, previously not reported, enables very sensitive and selective detection of plastochromanol which under the described separation conditions did not interfere with tocochromanols or any other compounds. The sample extraction method is fast, simple and highly efficient. The obtained results show that plastochromanol was present in most of the investigated seed oils. Its level was the highest in flax (17–30 mg/100 g oil), rape (8.5–9), camelina (4.3), peanut (1.95), corn (1.69) and grape (1.31) seed oils. Its level in the other investigated oils was below 1 mg/100 g oil, and only in sesame and coconut oils it was not detected. Tocotrienols were found in most of the oils but their content was usually very low (<<1 mg/100 g oil) with the exception of grape, milk thistle and corn oils where it reached >1 mg/100 g oil. Tocopherol content and isomer composition was within the earlier reported literature values for the investigated oils.

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Ackowledgments

This work was supported by the grant N302 049 32 obtained from the Polish Ministry of Science and Education.

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  1. Department of Plant Physiology and Biochemistry, Faculty of Biotechnology, Jagiellonian University, ul. Gronostajowa 7, 30-387, Kraków, Poland

    Jolanta Gruszka & Jerzy Kruk

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  1. Jolanta Gruszka
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  2. Jerzy Kruk
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Correspondence to Jerzy Kruk.

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Gruszka, J., Kruk, J. RP-LC for Determination of Plastochromanol, Tocotrienols and Tocopherols in Plant Oils. Chroma 66, 909–913 (2007). https://doi.org/10.1365/s10337-007-0416-2

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  • DOI: https://doi.org/10.1365/s10337-007-0416-2

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