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Composition of essential oil in genetically transformed roots of Ruta graveolens

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Abstract

The composition of essential oil obtained by hydrodistillation from genetically transformed roots of common rue (Ruta graveolens L.) was analyzed. Using gas chromatography and complex gas chromatography-mass spectrometry, it was established that the major component of rue essential oil was a root-specific sesquiterpene geijerene comprising 67% of total amount of volatile compounds. In essential oil of cultured rue roots, furocoumarins characteristic of intact plant roots were found, viz. osthole, halepensin, and rutacultin. The content of essential oil in genetically transformed rue roots was 0.23% of root dry weight, which is comparable with that in the roots of intact plants. The long-term maintenance in the in vitro cultured rue roots of a capability for the synthesis of essential oil major components characteristic of intact plants allows their usage for studying the physiological activity of these volatile compounds and their putative role in the plant root interaction in biocenoses.

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Abbreviations

Rt :

retention time.

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Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya ul. 35, Moscow, 127276, Russia

    I. N. Kuzovkina

  2. Department of Pharmacognosy, Semmelweis University, 1085, Budapest, Hungary

    Sz. Szarka, É. Héthelyi, E. Lemberkovics & É. Szöke

Authors
  1. I. N. Kuzovkina
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  2. Sz. Szarka
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  3. É. Héthelyi
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  4. E. Lemberkovics
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  5. É. Szöke
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Correspondence to I. N. Kuzovkina.

Additional information

Original Russian Text © I.N. Kuzovkina, Sz. Szarka, É. Héthelyi, E. Lemberkovics, É. Szöke, 2009, published in Fiziologiya Rastenii, 2009, Vol. 56, No. 6, pp. 935–941.

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Kuzovkina, I.N., Szarka, S., Héthelyi, É. et al. Composition of essential oil in genetically transformed roots of Ruta graveolens . Russ J Plant Physiol 56, 846–851 (2009). https://doi.org/10.1134/S1021443709060156

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  • DOI: https://doi.org/10.1134/S1021443709060156

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